Overexpression, Purification, Characterization, and Pathogenicity of
            <i>Vibrio harveyi</i>
            Hemolysin VHH

Zhong, Yueyang; Zhang, Xiaohua; Chen, Jixiang; Chi, Zhenghao; Sun, Bo; Li, Yun; Austin, Brian

doi:10.1128/iai.00512-06

Cited by 56 publications

(62 citation statements)

References 30 publications

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“…Thermolabile hemolysin (TLH) of V. parahaemolyticus, also named lecithin-dependent hemolysin (LDH), which had phospholipase A2/lysophospholipase activity (20), represents a major type of hemolysin in vibrios (24). In previous work, we obtained purified recombinant V. harveyi hemolysin (VHH) protein and showed that it was cytotoxic to flounder gill cells in tissue culture, had strong pathogenicity to flounder when injected intraperitoneally, and had phospholipase activity on egg yolk agar (26). In this study, we compared the characteristics and activities of VHH and the null-mutant protein of VHH obtained via site-directed mutagenesis.…”

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confidence: 99%

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

et al. 2007

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confidence: 99%

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

et al. 2007

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“…The V. harveyi hemolysin VHH is a PLB active against PC, hemolytic for fish erythrocytes, cytotoxic for fish cells, and upon intraperitoneal injection in flounder induces hemorrhage and death (93)(94)(95).…”

Section: Acyl Hydrolases: Phospholipases a And Bmentioning

confidence: 99%

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors

Flores-Dı́az

Monturiol-Gross

Naylor

et al. 2016

Microbiol Mol Biol Rev

173

143

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SUMMARYBacterial sphingomyelinases and phospholipases are a heterogeneous group of esterases which are usually surface associated or secreted by a wide variety of Gram-positive and Gram-negative bacteria. These enzymes hydrolyze sphingomyelin and glycerophospholipids, respectively, generating products identical to the ones produced by eukaryotic enzymes which play crucial roles in distinct physiological processes, including membrane dynamics, cellular signaling, migration, growth, and death. Several bacterial sphingomyelinases and phospholipases are essential for virulence of extracellular, facultative, or obligate intracellular pathogens, as these enzymes contribute to phagosomal escape or phagosomal maturation avoidance, favoring tissue colonization, infection establishment and progression, or immune response evasion. This work presents a classification proposal for bacterial sphingomyelinases and phospholipases that considers not only their enzymatic activities but also their structural aspects. An overview of the main physiopathological activities is provided for each enzyme type, as are examples in which inactivation of a sphingomyelinase- or a phospholipase-encoding gene impairs the virulence of a pathogen. The identification of sphingomyelinases and phospholipases important for bacterial pathogenesis and the development of inhibitors for these enzymes could generate candidate vaccines and therapeutic agents, which will diminish the impacts of the associated human and animal diseases.

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“…The V. harveyi haemolysin gene (vhhA) was overexpressed, purified and characterized by Zhong et al (2006). Evident phospholipase and haemolytic activities against turbot erythrocytes were determined for this protein.…”

Section: Haemolysins and Phospholipasesmentioning

confidence: 99%

“…Evident phospholipase and haemolytic activities against turbot erythrocytes were determined for this protein. In addition, the protein was proved cytotoxic for flounder gill cells as well as lethal for flounder (Zhong et al, 2006). Later, Sun et al (2007) carried out the construction of a vhhA site-directed mutant strain.…”

Section: Haemolysins and Phospholipasesmentioning

confidence: 99%

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Méndez¹,

Reimundo²,

Pérez-Pascual³

et al. 2012

Health and Environment in Aquaculture

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Overexpression, Purification, Characterization, and Pathogenicity of Vibrio harveyi Hemolysin VHH

Cited by 56 publications

References 30 publications

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors

An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

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