Overlooked? Underestimated? Effects of Substrate Curvature on Cell Behavior

Abstract

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Microcarriers for Upscaling Cultured Meat Production

“…Although other studies have also reported effects of curvature on several cell types including fibroblasts (31,95), osteoblastic cells (96,97) and MSCs (31)(32)(33), there is a lack of information regarding satellite cells, thus further investigation is still needed. With the increasing development of tools for the fabrication of micro-curved surfaces, more systematic and precise studies should be possible (98).…”

Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues

“…Many epithelial tissues exhibit complex morphologies that are mostly dominated by curved surfaces such as those found in lung alveoli and intestinal villi (Torras et al, 2018;Baptista et al, 2019). These three-dimensional (3D) topographies generate gradients in biochemical signals and mechanical tension that play a key role in cell polarization, morphology, and function (Farin et al, 2016;Krndija et al, 2019).…”

“…While traditionally ignored in flat in vitro models, 3D topographies are distinctive elements in the emergence and further development of a new generation of tissue-engineered cell culture substrates (Griffith and Swartz, 2006). These 3D models are capable of capturing the complex physiological responses of tissue in vitro and have been employed to systematically study the effects of in vivo-like tissue geometry and curvature on cell fate (Griffith and Swartz, 2006;Baptista et al, 2019).…”

Polylactic acid, a sustainable, biocompatible, transparent substrate material for Organ-On-Chip, and Microfluidic applications

“…Kinematic descriptors such as speed, mean curvature, and angular speed exhibit were extracted and compared for a PS Petri dish, PLA disc or inside a device. The kinematic descriptors between the PLA substrate and the PS were very similar despite slight difference on the surface topology [25]. However, the diffusion coefficient that is typical of a kind of motion and not strictly related to the kinematics of individual cells is equal for the two videos, thus demonstrating that cells under the microscope have the same behaviour (Fig 5B.iv).…”