Overview of new developments in and the future of cryopreservation in the laboratory mouse

Guan, Ming; Marschall, Susan; Raspa, Marcello; Pickard, Amanda R.; Fray, Martin

doi:10.1007/s00335-012-9423-1

Cited by 12 publications

(6 citation statements)

References 60 publications

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“…A great number of genetically engineered mice have been archived in mouse resource banks and are available from the banks via the website of International Mouse Strain Resource [ 1 ]. In the mouse resource bank, various reproductive techniques are used to efficiently preserve, transport, or produce genetically engineered mice [ 2 ]. Until now, a robust and efficient system for managing the mouse resource bank has been established using cryopreservation techniques for sperm, oocytes, and embryos [ 3 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice

2015

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Superovulation is a reproductive technique generally used to produce genetically engineered mice. Superovulation in mice involves the administration of equine chorionic gonadotropin (eCG) to promote follicle growth and then that of human chorionic gonadotropin (hCG) to induce ovulation. Previously, some published studies reported that inhibin antiserum (IAS) increased the number of ovulated oocytes in ddY and wild-derived strains of mice. However, the effect of IAS on the C57BL/6 strain, which is the most widely used inbred strain for the production of genetically engineered mice, has not been investigated. In addition, the combined effect of IAS and eCG (IASe) on the number of ovulated oocytes in superovulation treatment has not been examined. In this study, we examined the effect of IAS and eCG on the number of ovulated oocytes in immature female mice of the C57BL/6 strain in superovulation treatment. Furthermore, we evaluated the quality of obtained oocytes produced by superovulation using IASe by in vitro fertilization (IVF) with sperm from C57BL/6 or genetically engineered mice. The developmental ability of fresh or cryopreserved embryos was examined by embryo transfer. The administration of IAS or eCG had a similar effect on the number of ovulated oocytes in C57BL/6 female mice. The number of ovulated oocytes increased to about 3-fold by the administration of IASe than by the administration of IAS or eCG alone. Oocytes derived from superovulation using IASe normally developed into 2-cell embryos by IVF using sperm from C57BL/6 mice. Fresh or cryopreserved 2-cell embryos produced by IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice normally developed into live pups following embryo transfer. In summary, a novel technique of superovulation using IASe is extremely useful for producing a great number of oocytes and offspring from genetically engineered mice.

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Section: Introductionmentioning

confidence: 99%

Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice

2015

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“…At least 54,600 mouse strains have been archived as cryopreserved embryos and sperm [35, 36], which are efficiently distributed to research institutes worldwide. Mice can be rapidly produced and analyzed and then re-archived [37, 38]. Oocyte cryopreservation facilitates the use of archived female germ cells in diverse applications such as introducing gene modifications using IVF with genetically modified sperm or for genome editing after IVF.…”

Section: Discussionmentioning

confidence: 99%

N-acetyl cysteine restores the fertility of vitrified–warmed mouse oocytes derived through ultrasuperovulation

2019

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Oocyte cryopreservation is useful for preserving fertility and storing genetic resources. However, the small number of oocytes acquired using conventional treatment to induce superovulation and the reduction of fertility due to cryopreservation represent significant problems. Herein, we vitrified the oocytes derived through high-yield superovulation using inhibin antiserum and equine chorionic gonadotropin (IAS + eCG: IASe) and examined the yield of cryopreserved oocytes and survival rates relative to those of vitrified–warmed mouse oocytes derived through conventional superovulation using equine chorionic gonadotropin (eCG). Furthermore, we investigated the effects of N-acetyl cysteine on the fertility and developmental potential of vitrified–warmed oocytes derived using IASe. Compared with eCG, IASe increased the yield of cryopreserved oocytes and achieved equivalent survival rates. N-acetyl cysteine (0.5 mM) increased the fertilization rate of vitrified–warmed oocytes derived using IASe. Vitrification decreased thiol levels in the zona pellucida (ZP), while warming followed by N-acetyl cysteine treatment increased free thiol levels in ZP. Moreover, N-acetyl cysteine treatment recovered zona hardening by cleaving disulfide bonds and promoting the expansion of ZP. Two-cell embryos derived via in vitro fertilization using N-acetyl cysteine developed into normal pups through embryo transfer. Therefore, we developed an efficient technique for the production of cryopreserved oocytes using IASe through superovulation and found that N-acetyl cysteine improves the fertility of vitrified–warmed oocytes by cleaving the disulfide bonds and promoting the expansion of ZP.

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“…The cryopreservation of preimplantation embryos collected from GM animals allows the long-term storage of valuable lines for an infinite period of time and the subsequent ability to regenerate a colony of live animals in the future (Guan et al, 2012). Cryopreserved embryos are protected from genetic mutation and drift and genetic contamination as well as most facility catastrophes and husbandry expenses are avoided.…”

Section: G Preservation Of Gm Animalsmentioning

confidence: 99%

Genetically Modified Animals

Pritchett-Corning

Landel

2015

Laboratory Animal Medicine

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Overview of new developments in and the future of cryopreservation in the laboratory mouse

Cited by 12 publications

References 60 publications

Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice

Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice

N-acetyl cysteine restores the fertility of vitrified–warmed mouse oocytes derived through ultrasuperovulation

Genetically Modified Animals

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