Overview of the Development, Applications, and Future Perspectives of Decellularized Tissues and Organs

Nakamura, Naoko; Kimura, Tsuyoshi; Kishida, Akio

doi:10.1021/acsbiomaterials.6b00506

Cited by 76 publications

(58 citation statements)

References 68 publications

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“…Upon the removal of the cells, the shape, structure and components can be maintained and have shown promising results in vitro and in vivo. 79 However, decellularization methods can damage the composition, resulting in dysfunction or even a loss of important ECM components. Since the composition is hard to control, synthetic alternatives are proposed to provide a better solution.…”

Section: Natural Hydrogelsmentioning

confidence: 99%

From supramolecular polymers to multi-component biomaterials

et al. 2017

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The most striking and general property of the biological fibrous architectures in the extracellular matrix (ECM) is the strong and directional interaction between biologically active protein subunits. These fibers display rich dynamic behavior without losing their architectural integrity. The complexity of the ECM taking care of many essential properties has inspired synthetic chemists to mimic these properties in artificial one-dimensional fibrous structures with the aim to arrive at multi-component biomaterials. Due to the dynamic character required for interaction with natural tissue, supramolecular biomaterials are promising candidates for regenerative medicine. Depending on the application area, and thereby the design criteria of these multi-component fibrous biomaterials, they are used as elastomeric materials or hydrogel systems. Elastomeric materials are designed to have load bearing properties whereas hydrogels are proposed to support in vitro cell culture. Although the chemical structures and systems designed and studied today are rather simple compared to the complexity of the ECM, the first examples of these functional supramolecular biomaterials reaching the clinic have been reported. The basic concept of many of these supramolecular biomaterials is based on their ability to adapt to cell behavior as a result of dynamic non-covalent interactions. In this review, we show the translation of one-dimensional supramolecular polymers into multi-component functional biomaterials for regenerative medicine applications.

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Section: Natural Hydrogelsmentioning

confidence: 99%

From supramolecular polymers to multi-component biomaterials

et al. 2017

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“…Therefore, new engineering approaches have been devised based on the decellularization of tissues from different sources, aiming to produce scaffold materials that will not only act as fillers but will also integrate with the host surrounding tissue and promote its regeneration. This has led to the development of numerous commercial dECM products for soft tissue defect repair . While these products offer a native filler material, often preserving the innate tissue vasculature thereby promoting graft integration in the host, they exhibit limited reproducibility being derived from individual organs, and cannot be significantly modified in terms of their dimensions, composition, delivery mode, and 3D ultrastructure.…”

Section: Applications Of Processed Decm‐based Materialsmentioning

confidence: 99%

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Krishtul

Baruch

Machluf

2019

Adv Funct Materials

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Tissue-derived decellularized extracellular matrices (dECM) have gradually become the gold standard of scaffolds for tissue engineering, owing to their close mirroring of the intricate composition, architecture, and topology of the native extracellular matrix (ECM). Intriguingly, further manipulation of these acellular tissues through various processing techniques has been demonstrated to be an effective strategy to control their characteristics and impart them with ample valuable new traits, thereby expanding their applicability to a significantly wider spectrum of research and translational applications. Herein, state-of-the-art processed dECM platforms and their potential applications are focused on. The ECM characteristics that make it so appealing for tissue engineering are presented, followed by a concise discussion on the main considerations for choosing a dECM source for such applications. The key methodologies for dECM processing, including hydrogel production, bioprinting, electrospinning, and production of porous scaffolds, microcarriers, and microcapsules, as well as their inherent advantages and challenges, are introduced. To demonstrate the use of processed dECM platforms for tissue engineering, selected in vivo and in vitro applications recently developed utilizing these platforms are highlighted. Finally, concluding remarks and a prospective outlook for future developments and improvements in the field of processed dECM-based devices are given.

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“…The preparation of natural biological scaffolds involves a process known as decellularization as shown in Figure 1. Decellularization is a process that removes whole cellular components within the existing tissue while preserving the composition, integrity and mechanics of the three dimensional extracellular matrix scaffolds to the extent possible [23,24]. The elimination of the antigens and cellular components from the tissue-derived scaffolds able to reduce the potential immune rejection and inflammation from occurring [25].…”

Section: Decellularization Strategiesmentioning

confidence: 99%

Bio-Engineered Meniscus for Tissue Engineering

Azhim¹,

Ibrahim²,

Yusof³

2019

Meniscus of the Knee - Function, Pathology and Management

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Meniscus plays fundamental roles in the knee mechanisms and functions. It acts as a shock absorber where it enables even distribution of forces, and also lubricates knee joints. Meniscal injuries could result to the onset of degenerative osteoarthritis if proper treatments are delayed. To date, treatment of meniscal injuries are more towards conservative methods and surgical approach commonly known as meniscectomy. Attempts to develop scaffolds for meniscus implants from synthetic and biological sources have been done in the recent years. This approach involves a multidisciplinary study known as tissue engineering and regenerative medicine. It involves the combination of three crucial aspects; the choice of chondrogenic/stem cells, bioscaffolds and favourable environmental factors such as growth factors. This chapter discusses and highlights on the currently available meniscal scaffolds that have been explored before. Focus is also directed on the potential of decellularized extracellular matrix (ECM), prepared through sonication treatment that produced scaffolds which mimics natural meniscus. The evaluation of decellularized scaffolds was portrayed through recellularization using cells namely chondrocytes, fibrochondrocytes and stem cells in order to regenerate new functional tissue. In short, this chapter serves as a representation of current approaches aiming in bio-engineering the meniscal scaffolds as meniscus tissue replacement.

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Overview of the Development, Applications, and Future Perspectives of Decellularized Tissues and Organs

Cited by 76 publications

References 68 publications

From supramolecular polymers to multi-component biomaterials

From supramolecular polymers to multi-component biomaterials

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Bio-Engineered Meniscus for Tissue Engineering

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