Overview on microfluidics devices for monitoring brain disorder biomarkers

Nix, Cindy; Ghassemi, Maryam; Crommen, Jacques; Fillet, Marianne

doi:10.1016/j.trac.2022.116693

Cited by 15 publications

(7 citation statements)

References 78 publications

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“…Since the current response has submillisecond resolution and high sensitivity, microelectrode amperometric analysis was performed for conditions such as single cell analysis and single exocytotic reaction. 214 Tosar et al have revealed an amperometry process for determining the exosome. Rabbit polyclonal α-human CD9 antibodies were modified on substrates to detect exosomes and mouse monoclonal α-human CD9 antibodies attached to the residual CD9 fragments exposed on the exosome surface.…”

Section: ■ Exosome Detection By Sensorsmentioning

confidence: 99%

“…The increase or decrease of the current signal proportional to the reaction rate indicates the reduction or oxidation reactions. Since the current response has submillisecond resolution and high sensitivity, microelectrode amperometric analysis was performed for conditions such as single cell analysis and single exocytotic reaction . Tosar et al have revealed an amperometry process for determining the exosome.…”

Section: Exosome Detection By Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Exploring the Versatility of Exosomes: A Review on Isolation, Characterization, Detection Methods, and Diverse Applications

Altıntaş,

Saylan

2023

Anal. Chem.

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Extracellular vesicles (EVs) are crucial mediators of intercellular communication and can be classified based on their physical properties, biomolecular structure, and origin. Among EVs, exosomes have garnered significant attention due to their potential as therapeutic and diagnostic tools. Exosomes are released via fusion of multivesicular bodies on plasma membranes and can be isolated from various biofluids using methods such as differential ultracentrifugation, immune affinity capture, ultrafiltration, and size exclusion chromatography. Herein, an overview of different techniques for exosome characterization and isolation, as well as the diverse applications of exosome detection, including their potential use in drug delivery and disease diagnosis, is provided. Additionally, we discuss the emerging field of exosome detection by sensors, which offers an up-and-coming avenue for point-of-care diagnostic tools development. Overall, this review aims to provide a exhaustive and up-to-date summary of the current state of exosome research.

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Section: ■ Exosome Detection By Sensorsmentioning

confidence: 99%

Section: Exosome Detection By Sensorsmentioning

confidence: 99%

Exploring the Versatility of Exosomes: A Review on Isolation, Characterization, Detection Methods, and Diverse Applications

Altıntaş,

Saylan

2023

Anal. Chem.

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“…[53] a. Repeatability expresses the degree to which independent results obtained using the same procedure on identical samples concur with one another under the same settings (operator, laboratory, and after short intervals of time) b. Reproducibility expresses the closeness of agreement between independent results obtained with the same method on identical samples but under different conditions (analysts, laboratories, and/or after different intervals of time). [54] Precision can be expressed in terms of variance, standard deviation, or the coefficient of variation (CV). The smaller the variance or standard deviation, the higher the precision.…”

Section: Analytical Figures Of Meritmentioning

confidence: 99%

Enhancement in the limit of detection of lab‐on‐chip microfluidic devices using functional nanomaterials

2023

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Technological developments in recent years have witnessed a paradigm shift towards lab‐on‐chip devices for various diagnostic applications. Lab‐on‐chip technology integrates several functions typically performed in a large‐scale analytical laboratory on a small‐scale platform. These devices are more than the miniaturized versions of conventional analytical and diagnostic techniques. The advances in fabrication techniques, material sciences, surface modification strategies, and their integration with microfluidics and chemical and biological‐based detection mechanisms have enormously enhanced the capabilities of these devices. The minuscule sample and reagent requirements, capillary‐driven pump‐free flows, faster transport phenomena, and ease of integration with various signal readout mechanisms make these platforms apt for use in resource‐limited settings, especially in developing and underdeveloped parts of the world. The microfluidic lab‐on‐a‐chip technology offers a promising approach to developing cost‐effective and sustainable point‐of‐care testing applications. Numerous merits of this technology have attracted the attention of researchers to develop low‐cost and rapid diagnostic platforms in human healthcare, veterinary medicine, food quality testing, and environmental monitoring. However, one of the major challenges associated with these devices is their limited sensitivity or the limit of detection. The use of functional nanomaterials in lab‐on‐chip microfluidic devices can improve the limit of detection by enhancing the signal‐to‐noise ratio, increasing the capture efficiency, and providing capabilities for devising novel detection schemes. This review presents an overview of state‐of‐the‐art techniques for integrating functional nanomaterials with microfluidic devices and discusses the potential applications of these devices in various fields.

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“…Droplet-based microfluidic devices are critical in research areas such as bioanalysis (Sharma & Sharma 2022;Xu et al 2022;Sun et al 2023), disease surveillance (Giuffrida, Cigliana & Spoto 2018;Nix et al 2022), the synthesis of nanomaterials (Zou et al 2022;Gimondi et al 2023;Yao et al 2023) and microchemical reactors (Whitesides 2006;Verma & Pandya 2022). In these areas, droplets with controlled volume and good uniformity are prerequisites for ensuring device accuracy and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…In practical applications, fabrication defects inevitably exist in microchannels, which not only result in asymmetry between the two branch channels, but also generate daughter droplets of different volumes. Although active agents or surfactants are known to promote droplet breakup and prevent droplets from coalescing (Baret et al 2009;Pan et al 2016), their effects on the disturbed flow field, neck contraction process and enhancement or weakening of the disturbances caused by tiny deficiencies and defects are still unclear. Therefore, it is necessary to understand the effect of surfactants on the passive breakup of a droplet in a T-junction microchannel, which is helpful for controlling the surfactant dosage and generating droplets with controlled volume.…”

Section: Introductionmentioning

confidence: 99%

Modelling the passive breakup of a surfactant-contaminated droplet in a T-junction microchannel

Zhang,

Wang,

Chen

et al. 2024

J. Fluid Mech.

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A lattice Boltzmann method is used to explore the effect of surfactants on the unequal volume breakup of a droplet in a T-junction microchannel, and the asymmetry due to fabrication defects in real-life microchannels is modelled as the pressure difference between the two branch outlets ( $\Delta {P^\ast }$ ). We first study the effect of the surfactants on the droplet dynamics at different dimensionless initial droplet lengths ( $l_0^\ast $ ) and capillary numbers (Ca) under symmetric boundary conditions ( $\Delta {P^\ast } = 0$ ). The results indicate that the presence of surfactants promotes droplet deformation and breakup at small and moderate $l_0^\ast $ values, while the surfactant effect is weakened at large $l_0^\ast $ values. When the branch channels are completely blocked by the droplet, a linear relationship is observed between the dimensionless droplet length ( $l_d^\ast $ ) and dimensionless time ( ${t^\ast }$ ), and two formulas are proposed for predicting the evolution of $l_d^\ast $ with ${t^\ast }$ for the two systems. We then investigate the effect of the surfactants on the droplet breakup at different values of $\Delta {P^\ast }$ and bulk surfactant concentrations ( ${\psi _b}$ ) under asymmetric boundary conditions ( $\Delta {P^\ast } \ne 0$ ). It is observed that, as $\Delta {P^\ast }$ increases, the volume ratio of the generated droplets ( ${V_1}/{V_2}$ ) decreases to 0 in both systems, while the rate of decrease is higher in the clean system, i.e. the presence of surfactants could cause a decreased pressure difference between the droplet tips. As ${\psi _b}$ increases, ${V_1}/{V_2}$ first increases rapidly, then remains almost constant and finally decreases slightly. We thus establish a phase diagram that describes the ${V_1}/{V_2}$ variation with $\Delta {P^\ast }$ and ${\psi _b}$ .

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Overview on microfluidics devices for monitoring brain disorder biomarkers

Cited by 15 publications

References 78 publications

Exploring the Versatility of Exosomes: A Review on Isolation, Characterization, Detection Methods, and Diverse Applications

Exploring the Versatility of Exosomes: A Review on Isolation, Characterization, Detection Methods, and Diverse Applications

Enhancement in the limit of detection of lab‐on‐chip microfluidic devices using functional nanomaterials

Modelling the passive breakup of a surfactant-contaminated droplet in a T-junction microchannel

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