Ovulation Rates and Litter Sizes in Sexually Receptive and Nonreceptive Artificially Inseminated Rabbits Given Varying Dosages of Luteinizing Hormone

Foote, R.H.; Hafs, H. D.; Staples, Robert; Gregoire, A. T.; Bratton, R.W.

doi:10.1530/jrf.0.0050059

Cited by 21 publications

(8 citation statements)

References 15 publications

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“…The difference may be due to the fact that the cumulative control data were obtained during the various seasons occurring over a period of 1^years. A single intravenous injection of lh into the mature Dutch-Belted doe was previously reported to result in ovulation in virtually every animal (Foote, Hafs, Staples, Grégoire & Bratton, 1963). After similar lh administration to thirty-two smaller Dutch-Belted does in this study, only eleven had cl when laparotomized 18 days later.…”

supporting

confidence: 52%

Influence of Body Weight Upon Corpus Luteum Formation and Maintenance of Pregnancy in the Rabbit

Staples¹,

Holtkamp²

1966

Reproduction

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Mature female rabbits readily ovulate and form functional corpora lutea (cl) if given exogenous luteinizing hormone (lh). lh was routinely administered to artificially inseminated Dutch-Belted does to initiate pregnancies successfully for teratology experiments (Staples & Holtkamp, 1963) ; however, it was noted that fewer pregnancies resulted among small, supposedly mature, does obtained from commercial suppliers. It was realized that, if body weight was related to response to lh administration among such small does of unknown age, a guideline was necessary for pre-selection of responding from non-responding does. This study was conducted to provide an estimate of the incidence of l h\ x=req-\ induced cl formation and maintenance of pregnancy among supposedly mature does of varied weight groups.Thirty-two young, virgin, Dutch-Belted does were individually caged for 18 days. Body weights were recorded and each doe was injected intravenously with 1\m=.\0 mg of LH/kg (PLH, Armour). Laparotomies were performed 18 days later and the ovaries were examined for the presence or absence of cl. On the following day body weights were recorded, and twenty-six of the does were again injected with lh and then artificially inseminated. On the 25th day after insemination laparotomies were performed. Adams (1960) reports that such laparotomies do not increase prenatal mortality. Three days later body weights were again taken, and the eighteen non-pregnant does were given lh and artificially inseminated. All eighteen does were autopsied 30 days later and their reproductive status determined. Point biserial coefficients (Edwards, 1954) were calculated to determinate the relationships between body weight and incidence of cl or pregnancy. The relationship between body weight and litter size (Table 1) was determined by regression analysis (Snedecor, 1956).The effect of body weight upon the incidence of cl formation (Text- fig. 1) was highly significant (P<0\m=.\01). cl were nevertheless present in two of twelve does that weighed between 0\m=.\95and 1\m=.\14 kg. One of these two does weighed only 1 \m=.\05 kg and maintained two foetuses with a total of only two cl.In Table 1 the number of does with cl and the number pregnant is presented, Present address: The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts. h 221

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confidence: 52%

Influence of Body Weight Upon Corpus Luteum Formation and Maintenance of Pregnancy in the Rabbit

Staples¹,

Holtkamp²

1966

Reproduction

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“…Templeton (1940) found that force mating of non-receptive females caused only two of five to ovulate, and only five of twenty does maintained a pregnancy to term if force bred to an intact male 17 or more days after a sterile mating. A low incidence of ovulation after force mating was also reported by Foote, Hafs, Staples, Grégoire & Bratton (1963). Mere rubbing of the vulva was reported to cause ovulation in two of four attempts ; how¬ ever, this finding was not confirmed by Shibata (1931).…”

Section: Introductionmentioning

confidence: 64%

Behavioural Induction of Ovulation in the Oestrous Rabbit

Staples¹

1967

Reproduction

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The incidence of ovulation was determined among oestrous Dutch-belted' does following exposure to varying degrees of behavioural stimulation short of coitus. The mounting of does by a male did not induce ovulation even though on more than 240 occasions the females assumed the fully arched position during which vaginal penetration was prevented by deflection of the males' hind quarters. Receptive females did not ovulate after thirty-five exposures as teasers for semen collection by artificial vagina, or if retained in close proximity to the male in addition to being mounted once daily over a 2-week period. Ovulation did occur among submissive oestrous does if they were repeatedly mounted by oestrous or anoestrous does while caged in pairs for periods ranging from 1 to 17 days, but ovulation was not observed among females that assumed only the dominant role.

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“…The ovulation inducing method most frequently used is an intramuscular injection of GnRH or its synthetic analogues [4][5][6]. Alternatively, LH or hCG can be used to induce ovulation in rabbit does [7], however, the repeated injection of these hormones can result in failure of the does to ovulate due to antibody formation [8]. Vasectomised males have been used to induce ovulation in does submitted to AI [9,10], but the method is time-consuming and some females refuse to mate, but ovulate when given LH or GnRH.…”

Section: Introductionmentioning

confidence: 99%

Ovulation induction in rabbit does submitted to artificial insemination by adding buserelin to the seminal dose

et al. 2004

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-This study was aimed at determining if a GnRH analogue, buserelin, could be used for ovulation induction in rabbit does submitted to artificial insemination (AI) by intravaginal administration, by adding the hormone to the seminal dose. In a first experiment, 39 secondiparous experimental does (Hyplus strain PS19, Grimaud Frères, France, of about 30 weeks of age) were divided into 3 groups of 13 does each, which at the moment of AI received the following treatments, respectively: (1) control: an intramuscular injection of buserelin (0.8 µg/doe), (2) 8 µg/doe of buserelin added to the insemination dose, and (3) 16 µg/doe of buserelin added to the insemination dose. The experiment was done using 3 consecutive cycles at 42 day-intervals (n = 39). Four does from each of the 3 groups had blood taken at the fourth cycle for LH determination at 0, 60, 90, 120 and 150 min relative to AI. Kindling rates were 82% (28/34), 56% (29/36) and 85% (33/39), respectively for treatments 1, 2 and 3. In the does of groups 2 and 3, LH peaks were detected 60 min after AI, whereas in the does from group 1, the LH peak was detected 90 min after AI. Prolificacy was not different for the 3 treatments (average litter sizes ranged from 10.4 to 10.8). In a second experiment, 3 buserelin concentrations (8, 12 and 16 µg/doe) were used intravaginally and compared with the control treatment (0.8 µg/doe, via intramuscular). This experiment was done using 100 nulliparous rabbit does (Hyplus strain PS19, Grimaud Frères, France, of about 19 weeks of age) (4 groups of 25 does each) located on a commercial farm, to test if the previous results would be confirmed under field conditions. Kindling rates were no different (P < 0.05) for the 4 treatment groups [91.7% (22/24), 79.2% (19/24), 87.0% (20/23) and 87.5% (21/24) respectively for the control, 8, 12 and 16 µg of intravaginal buserelin], however, prolificacy was higher when using the maximal dose of intravaginal buserelin (11.7 vs. 9.4 for the control group). It was concluded that buserelin can be used for ovulation induction in rabbit does when included in the seminal dose, with similar AI results as those obtained when the hormone is administered intramuscularly.

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Ovulation Rates and Litter Sizes in Sexually Receptive and Nonreceptive Artificially Inseminated Rabbits Given Varying Dosages of Luteinizing Hormone

Cited by 21 publications

References 15 publications

Influence of Body Weight Upon Corpus Luteum Formation and Maintenance of Pregnancy in the Rabbit

Influence of Body Weight Upon Corpus Luteum Formation and Maintenance of Pregnancy in the Rabbit

Behavioural Induction of Ovulation in the Oestrous Rabbit

Ovulation induction in rabbit does submitted to artificial insemination by adding buserelin to the seminal dose

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