Oxidase Reaction

Rogers, K.B.

doi:10.1016/s0140-6736(63)90475-6

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…Steel 1961) on wet (Kovacs 1956) or dry (Rogers 1963) paper. Ascorbic acid added to the test reo agent (Steel 1962) was useful, because it reduced the number of doubtfully positive reactions.…”

Section: Resultsmentioning

confidence: 99%

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A taxonomic study of plant pathogenicCorynebacteriumspecies

Dye

Kemp

1977

New Zealand Journal of Agricultural Research

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Methods that have been developed and standardised in our laboratory for the determination of cultural and biochemical characters of plant pathogenic bacteria have been used to provide data for numerical analyses of 121 strains of 13 phytopathogenic species of Corynebacterium Lehmann & Neumann 1896. Our results show them to be a relatively homogeneous group of organisms which should be classified within a single genus. Other workers have made proposals to exclude plant pathogenic coryneform species from Corynebacterium, but we consider that detailed comparative evidence is insufficient to justify their transfer to any other genus. However, we cannot support the present division of coryneform plant pathogens into 13 species, and propose the following classification.

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Section: Resultsmentioning

confidence: 99%

“…Both Rogers' (1963) modification and the method of Kovacs (1956) were used to test for oxidase with both nichrome and platinum loops. The use of 0.1% ascorbic acid as recommended by Steel (1962) was also tested.…”

Section: Oxidasementioning

confidence: 99%

A taxonomic study of plant pathogenicCorynebacteriumspecies

Dye

Kemp

1977

New Zealand Journal of Agricultural Research

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“…The oxidase test is an important differential procedure in diagnostic bacteriology, especially useful for quickly confirming a tentative morphological identification of Pseudomonas aeruginosa or Neisseria species. This can be done as a filter paper spot test (5)(6)(7)(8) or by flooding colonies with one of several reagents, with or without alphanaphthol (3, 4). When fresh solutions of the reagents are used, the test results are quite reliable, but variable results may be obtained if the solutions are not prepared frequently.…”

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confidence: 99%

“…Such moist papers must be replaced after a few hours because the reagent becomes oxidized rapidly. Alternatively, reagent-impregnated filter paper can be dried and stored for prolonged periods of time I Present address: University of California at Davis, School of Medicine, Department of Medicine, Davis, 95616. with no loss of activity (6,7). Small filter paper discs containing p-aminodimethylaniline are available from Difco and BBL.…”

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confidence: 99%