Oxidative and DNA Damage Potential of Colemanite on Zebrafish: Brain, Liver and Blood

Abstract

<jats:p>Recently, boron has been used in animal feeding due to its significant biological roles. In this study, the action mechanism of colemanite (COL), a commercially important borate mineral, was aimed to investigate via evaluating parameters related to oxidative alterations on the brain, liver and blood tissues of zebrafish. For this purpose, zebrafish were exposed to different doses of COL (5, 10 and 20 mg/L) in a static test apparatus for 96 hours. Multiple biochemical analysis including determination of DNA damage (8-OHdG), apoptosis (Caspase-3), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), myeloperoxidase (MPO), paraoxonase (PON), arylesterase (AR) and lipid peroxidation (MDA) levels were performed in brain and liver tissues for assessing oxidative responses. In addition to micronucleus (MN) assay was performed in obtained blood tissues. The results indicated that low doses of COL supported antioxidant system and did not lead to oxidative stress in zebrafish brain and liver. Again, our results showed colemanite did not cause DNA damage or apoptosis at all tested concentrations. Besides the statistically insignificant changes (P&gt;0.05) of MN rates of erythrocytes between the control and experimental groups revealed the non-genotoxic feature of COL on zebrafish. In conclusion, boron compounds especially COL can be used safely and provide positive impacts on aquatic environments.</jats:p>

Sindrome De Burnout en Personal De Enfermeria De Un Hospital Reconvertido a Covid-19

“…Tabela 2 -Frequência de eritrócitos com micronúcleos (MN) no grupo controle e no grupo exposto ao protocolo de estresse imprevisível Fonte: os autores ROCCO et al, 2012;KOVÁCS et al, 2015;DU et al, 2016;ALAK et al, 2020a;ALAK et al, 2020b) XAVIER et al, 2020;MAGNO et al, 2020).…”

Assessment of 8-hydroxy-2-deoxyguanosine activity, apoptosis, acetylcholinesterase and antioxidant enzyme activity in Capoeta umbla brain exposed to chlorpyrifos

“…It can be said that this change causes oxidative stress, DNA damage and apoptosis in fish. In addition, it can be considered that this increase occurs in apoptotic cells due to the deterioration of the body enzyme balance and the alteration of the apoptosis pathway (Alak et al 2020;Uçar et al 2020b).…”

Pre-probiotic effects of different bacterial species in aquaculture: behavioral, hematological and oxidative stress responses

“…Tissue samples (brain, gill, and liver) collected from fish were homogenized in a homogenizer after adding the KH 2 PO 4 buffer solution three times. The obtained homogenates were centrifuged at 4°C depending on the tissue type and the supernatants were used to measure enzyme activities (Alak et al 2020a). The AChE activity and MDA content in brain homogenates, and antioxidant defense indicators (SOD, CAT, GPx, GR, GST, G6PD activity or MDA content) in gill and liver homogenates were determined.…”

“…Evaluation of blood parameters is an important marker and a commonly used method in assessing the effects of similar therapeutics on fish health. With this technique, it is possible to make reliable decisions in determining the physiological state and environmental conditions of an animal (Uçar et al 2019;Alak et al 2020a;Uçar et al 2021). By studying the hematological parameters, effects of different social, physiological and environmental factors (such as social hierarchy, disease, nutritional deficiencies, toxic substances, changes in water quality, temperature, photoperiod, density, salinity, pH, oxygen, heavy metals, pesticides, detergents) on fish health and physical state can be determined (Uçar et al 2019;Alak et al 2020b).…”