Oxidative damage causes formation of lipofuscin-like substances in the hippocampus of the senescence-accelerated mouse after kainate treatment

Kim, Hyoung Chun; Bing, Guoying; Jhoo, Wang Kee; Kim, Won Ki; Shin, Eun Joo; Park, Eon Sup; Choi, Yong; Lee, Dong Hoon; Shin, Chan Young; Ryu, Jae Ryun; Ko, Kwang Ho

doi:10.1016/s0166-4328(01)00382-5

Cited by 45 publications

(43 citation statements)

References 62 publications

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“…Using a convulsion meter (CONVULS-1, Columbus Instruments, Columbus, OH, USA) (Kim et al, 2002a), the seizure activity over a 20-min period was measured at 1 h, 2 h, 4 h, 1 day, 2 days, 3 days, 4 days, 1 week, 2 weeks, and 3 weeks after TMT treatment.…”

Section: Determination Of Seizure Activitymentioning

confidence: 99%

“…The amount of lipid peroxidation was determined by measuring the accumulation of thiobarbituric acid-reactive substances in homogenates of hippocampal tissue and is expressed as MDA content (Jareno et al, 1998;Kim et al, 1999bKim et al, , 2000cKim et al, , 2002a. In brief, 0.1 ml of the homogenate (or standard solutions prepared daily from 1,1,3,3-tetra-methoxypropane) and 0.75 ml of the working solution (thiobarbituric acid 0.37% and perchloric acid 6.4%, 2:1 v/v) were mixed and heated to 95°C for 1 h. After cooling (10 min in ice water bath), the flocculent precipitate was removed by centrifugation at 3200ϫg for 10 min.…”

Section: Determination Of Mdamentioning

confidence: 99%

“…The extent of protein oxidation was assessed by measuring the content of protein carbonyl groups, which was determined spectrophotometrically with the 2,4-dinitrophenylhydrazine (DNPH)-labeling procedure (Kim et al, 1997(Kim et al, , 2000c(Kim et al, , 2002a as described by Oliver et al (1987). The results are expressed as nmol of DNPH incorporated/mg protein based on the extinction coefficient (21 mM Ϫ1 cm Ϫ1 ) for aliphatic hydrazones.…”

Section: Determination Of Protein Carbonylmentioning

confidence: 99%

“…Only neurons with a visible nucleus and in which the entire outline of the cell was apparent were counted (Kim et al, 1999a;Shin et al, 2004Shin et al, , 2005. Every sixth section (200 m separation distance) was evaluated with a 63ϫ oil immersion objective on an Olympus microscope using a video camera and monitor (Kim et al, 1997(Kim et al, , 2000c(Kim et al, , 2002a.…”

Section: Cresyl Violet-staining and Neuron Countsmentioning

confidence: 99%

“…Abnormalities in glutathione synthesis can result in neurological disorders (Bains and Shaw, 1997;Schweizer et al, 2004), and recent studies have suggested that membrane lipid peroxidation may be involved in epileptogenesis in some experimental models of epilepsy (Kim et al, 1997(Kim et al, , 2000c(Kim et al, , 2002aSingh and Pathak, 1990;Willmore et al, 1986). The production of epileptiform discharges in some forms of experimental epilepsy/convulsion is believed to be mediated by membrane lipid peroxidation initiated by the action of oxygen radicals, which can alter electrophysiologic properties of membranes, such as iongating (Pellmar, 1986).…”

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confidence: 99%

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Ascorbate attenuates trimethyltin-induced oxidative burden and neuronal degeneration in the rat hippocampus by maintaining glutathione homeostasis

et al. 2005

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Section: Determination Of Seizure Activitymentioning

confidence: 99%

Section: Determination Of Mdamentioning

confidence: 99%

Section: Determination Of Protein Carbonylmentioning

confidence: 99%

Section: Cresyl Violet-staining and Neuron Countsmentioning

confidence: 99%

mentioning

confidence: 99%

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Ascorbate attenuates trimethyltin-induced oxidative burden and neuronal degeneration in the rat hippocampus by maintaining glutathione homeostasis

et al. 2005

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Distinct Types of Lipofuscin Pigment in the Hippocampus and Cerebellum of Aged Cheirogaleid Primates

Gilissen

Staneva-Dobrovski

2013

The Anatomical Record

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The formation of autofluorescent lipopigment or lipofuscin is a highly consistent and reliable cytological change that correlates with cellular aging in postmitotic cells. One causal factor of lipofuscinogenesis involves free radical-induced lipid peroxidation. In mammals, dentate gyrus neurons and Purkinje cells are usually affected widely. In this study, we investigated the ultrastructure of lipofuscin deposits in large neurons of the dentate gyrus and in Purkinje cells of aged fat-tailed dwarf lemurs (Cheirogaleus medius Geoffroy, 1812) with electron and confocal microscopy and compared it with previous observations in other species. Cheirogaleid primates such as mouse and dwarf lemurs are archaic primates that provide interesting nonhuman models of aging. Our study revealed region-specific as well as species-specific characteristics of lipofuscin ultrastructure. This suggests differences in cellular metabolism and/or in organelles involved in lipofuscin production in cerebellar Purkinje cells and in hippocampal dentate gyrus neurons.

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Beneficial effect of yam on the amyloid β‐protein, monoamine oxidase B and cognitive deficit in mice with accelerated senescence

et al. 2006

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This study was designed to evaluate the effect of yam tuber material [Dioscorea alata L. var. purpurea (M.) Pouch.] on the amyloid β-protein (Aβ) accumulation, monoamine oxidase (MAO) activity, cognitive ability and antioxidative defense system in senescence-accelerated mice (SAMP8). Three-month-old male mice were fed with four different diets for 12 weeks: a casein diet (control group) and a casein diet supplemented with either 100, 200 or 400 g kg −1 lyophilized yam. The results of the active shuttle avoidance test showed that the mice fed with the yam-containing diets had significantly better learning and memory ability than the control group. All yam-containing diet groups had lower Aβ levels and MAO B activities than the control, whereas the MAO A activity did not differ among the four diet groups. At the 400 g kg −1 level, the yam-containing diet group showed significantly lower concentration of thiobarbituric acid-reactive substances (TBARS), higher total thiol level and superoxide dismutase (SOD) activity in the hippocampus than the control, but these values were not altered by the 100 and 200 g kg −1 yam-containing diet groups. The addition of 200 or 400 g kg −1 yam also lowered the triglyceride levels but not the total cholesterol concentration. These results indicate that lyophilized yam could reduce brain Aβ accumulation, MAO B activity and cognitive deficits and promote the antioxidative defense system in SAMP8 mice. The improvements were in a dose-dependent manner, possibly because the 400 g kg −1 yam-containing diet might contain more antioxidative phytochemicals.

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Oxidative damage causes formation of lipofuscin-like substances in the hippocampus of the senescence-accelerated mouse after kainate treatment

Cited by 45 publications

References 62 publications

Ascorbate attenuates trimethyltin-induced oxidative burden and neuronal degeneration in the rat hippocampus by maintaining glutathione homeostasis

Ascorbate attenuates trimethyltin-induced oxidative burden and neuronal degeneration in the rat hippocampus by maintaining glutathione homeostasis

Distinct Types of Lipofuscin Pigment in the Hippocampus and Cerebellum of Aged Cheirogaleid Primates

Beneficial effect of yam on the amyloid β‐protein, monoamine oxidase B and cognitive deficit in mice with accelerated senescence

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