Oxidative damage effects in the copepod Tigriopus japonicus Mori experimentally exposed to nickel

Abstract

Tigriopus japonicus Mori has been recognized as a good model for toxicological testing of marine pollutants. Recently, a large number of genes have been identified from this copepod, and their mRNA expression has been studied independently against exposure to marine pollutants; however, biochemical-response information is relatively scarce. The response of T. japonicus to nickel (Ni) additions was examined under laboratory-controlled conditions in 12 days exposure. Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), acetylcholinesterase (AchE), reduced glutathione (GSH), the ratio of reduced to oxidized glutathione (GSH/GSSG) and metallothionein (MT) were analyzed for Ni treatments (0, 0.125, 0.25, 0.75 and 3.0 mg/L) after 1, 4, 7 and 12 days. The thiobarbituric reactive species assay was used to evaluate lipid peroxidation (LPO) level in copepods after exposure. The results showed that Ni remarkably affected the biochemical parameters (SOD, GPx, GST, GSH, and GSH/GSSG) after certain exposure durations. However, the copepod's LPO level was significantly decreased under metal treatments after exposure, hinting that the factors involved in LPO might not significantly depend on the operations and functions in the antioxidant system. Ni exhibited the neurotoxicity to copepods, because its use obviously elevated AchE activity. During exposure, Ni initially displayed an inhibition effect but induced MT synthesis in T. japonicus by day 12, probably being responsible for metal detoxification. Thus, Ni had intervened in the detoxification process and antioxidant system of this copepod, and it could be used as a suitable bioindicator of Ni exposure via measuring SOD, GPx, GST, and MT as biomarkers.

Nickel Chloride (NiCl2) Induces Histopathological Lesions via Oxidative Damage in the Broiler’s Bursa of Fabricius

“…**p < 0.01, compared with the control group or lesions [18][19][20][21][22]. Some studies have reported that NiCl 2 can decrease the activities of enzymic and non-enzymic antioxidants in rats, Tigriopus japonicus Mori, and goldfish [39][40][41]. Also, our data are consistent with the report that NiSO 4 can decrease the SOD and CAT activities in the liver of mouse [42].…”

Effects of pyrene on antioxidant systems and lipid peroxidation level in mangrove plants, Bruguiera gymnorrhiza

“…MDA, a decompression product of polyunsaturated fatty acids hydroperoxides, is considered as an indicator of ROS accumulation (Dotan et al 2004;Shi and Bao 2007;Wang and Wang 2009). In the present study, PAH stimulates MDA in roots and stems of B. gymnorrhiza, while MDA in leaves seems to be little affected by PAH in a range of concentration applied.…”

Impacts of mercury exposure on life history traits of Tigriopus japonicus: Multigeneration effects and recovery from pollution

“…The harpacticoid copepod Tigriopus japonicus has a wide geographical distribution and is regarded as a good model species commonly used in marine ecotoxicological studies (Raisuddin et al, 2007;Wang and Wang, 2010). This copepod is relatively small, the adults being approximately, 1.0 mm generally.…”