Oxidative DNA damage impairs global sperm DNA methylation in infertile men

Tunc, Ozlem; Tremellen, Kelton

doi:10.1007/s10815-009-9346-2

Cited by 212 publications

(186 citation statements)

References 55 publications

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“…A study of 45 infertile men found that seminal ROS production was negatively correlated with sperm global DNA methylation, and three-month supplementation with antioxidants produced a significant decrease in seminal ROS and significant increase in sperm global DNA methylation. 24 Additionally, in a study of 61 bladder cancer patients and 45 healthy controls, total antioxidant status in urine was positively correlated with methylation of long interspersed nuclear element-1 (LINE-1) in peripheral blood leukocyte (PBL) DNA from all subjects. 25 To our knowledge, this is the first study to find an association between blood glutathione redox status and global DNA methylation in a generally healthy population.…”

Section: Discussionmentioning

confidence: 99%

Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults

et al. 2013

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“…Proliferation and differentiation of the male germ cells can be disturbed at every level. This may occur as a result of influences that directly or indirectly affect spermatogenesis [9,10].…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma homocysteine, folate and cobalamin in men with obstructive and non-obstructive azoospermia

Crha

Králíková

Melounová

et al. 2010

J Assist Reprod Genet

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Purpose The aim of this study was to analyze homocysteine, folate and cobalamin in men with normozoospermia, obstructive and non-obstructive azoospermia. Methods Analysis of plasma and seminal plasma homocysteine, folate and cobalamin in 72 azoospermic and 62 normozoospermic men. Evaluation of the azoospermic patient included testicular biopsy, endocrine, urological and ultrasound examination. Results Homocysteine (1.2 μmol/l) and cobalamin (322.05 pmol/l) concentrations (median values) in seminal plasma were significantly lower (p<0.001) in men with azoospermia than in men with normozoospermia (2.5 μmol/ l and 579.0 pmol/l). Folate and cobalamin concentrations were significantly higher in obstructive than in nonobstructive azoospermia. Significant correlations were determined between testis volume and seminal plasma homocysteine in azoospermic men. Conclusion Lower concentrations of homocysteine and cobalamin (but not folate) were found in azoospermic seminal plasma than normozoospermic. Folate and cobalamin were higher in seminal plasma from obstructive azoospermia than in non-obstructive azoospermia patients.

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“…Another reason for reduced expression of PLCζ could be related to oxidative stress. DNA and RNA are common targets of reactive oxygen species [37] which might have a critical effect on embryo quality and pregnancy rates in IVF/ICSI treatments [38][39][40] and increased upon heat stress or in varicocele condition [30,41].…”

Section: Discussionmentioning

confidence: 99%

An association between sperm PLCζ levels and varicocele?

Janghorban-Laricheh

Ghazavi-Khorasgani

Tavalaee

et al. 2016

J Assist Reprod Genet

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Purpose We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele. Methods This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010. Sperm DNA fragmentation, relative expression of PLCζ at messenger RNA, and protein levels were evaluated by sperm chromatin structure assay (SCSA), real-time PCR, and Western blot analysis, respectively. Results The results of this study reveal that the mean relative expression of PLCζ was significantly lower in individuals with varicocele compared to fertile men at both transcription and translation levels. In addition, the percentage of DNA fragmentation was significantly higher in infertile men with varicocele compared to fertile men. Conclusions The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCζ. This effect could subsequently reduce the sperm ability to induce oocyte activation. Therefore, these results hold promise to modify our understanding of reproductive physiology of varicocele state.

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Oxidative DNA damage impairs global sperm DNA methylation in infertile men

Abstract: These results suggest that oxidative stress related damage to sperm DNA impedes the process of methylation, while antioxidant supplementation appears to have the potential to reduce DNA damage and normalize sperm DNA methylation.

Cited by 212 publications

References 55 publications

Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults

Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults

Seminal plasma homocysteine, folate and cobalamin in men with obstructive and non-obstructive azoospermia

An association between sperm PLCζ levels and varicocele?

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