Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Ritter, Cristiane; Andrades, Michael Éverton; Frota, Mario Luiz Conte da; Bonatto, Fernanda; Pinho, Ricardo A.; Polydoro, Manuela; Klamt, Fábio; Pinheiro, Cleovaldo T. S.; Menna-Barreto, Sérgio Saldanha; Moreira, José Cláudio Fonseca; Dal‐Pizzol, Felipe

doi:10.1007/s00134-003-1789-9

Cited by 171 publications

(142 citation statements)

References 41 publications

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“…The animals were subjected to CLP as previously described (19)(20)(21)(22). Briefly, rats were anesthetized with a mixture of ketamine (80 mg/kg) and xylazine (10 mg/kg), given intraperitoneally.…”

Section: Cecal Ligation and Perforation Surgerymentioning

confidence: 99%

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Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation

Barichello

Martins

Reinke

et al. 2007

Braz J Med Biol Res

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Sepsis and its complications are the leading causes of mortality in intensive care units, accounting for 10-50% of deaths. Intensive care unit survivors present long-term cognitive impairment, including alterations in memory, attention, concentration, and/or global loss of cognitive function. In the present study, we investigated behavioral alterations in sepsis-surviving rats. One hundred and ten male Wistar rats (3-4 months, 250-300 g) were submitted to cecal ligation and puncture (CLP), and 44 were submitted to sham operation. Forty-four rats (40%) survived after CLP, and all sham-operated animals survived and were used as control. Twenty animals of each group were used in the object recognition task (10 in short-term memory and 10 in long-term memory), 12 in the plus-maze test and 12 in the forced swimming test. Ten days after surgery, the animals were submitted individually to an object recognition task, plus-maze and forced swimming tests. A significant impairment of short-and long-term recognition memory was observed in the sepsis group (recognition index 0.75 vs 0.55 and 0.74 vs 0.51 for short-and long-term memory, respectively (P < 0.05). In the elevated plus-maze test no difference was observed between groups in any of the parameters assessed. In addition, sepsis survivors presented an increase in immobility time in the forced swimming test (180 vs 233 s, P < 0.05), suggesting the presence of depressive-like symptoms in these animals after recovery from sepsis. The present results demonstrated that rats surviving exposure to CLP, a classical sepsis model, presented recognition memory impairment and depressive-like symptoms but not anxietylike behavior.

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Section: Cecal Ligation and Perforation Surgerymentioning

confidence: 99%

“…In this context, murine models of cecal ligation and perforation (CLP) are clinically relevant since they induce a polymicrobial sepsis that mimics human sepsis (19)(20)(21)(22). The CLP model has contributed to the elucidation of the pathogenesis and to the determination of new therapies in sepsis (19,20).…”

Section: Introductionmentioning

confidence: 99%

Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation

Barichello

Martins

Reinke

et al. 2007

Braz J Med Biol Res

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“…29 Caecal ligation and perforation provides an alternative model in which bacteria invade the intraperitoneal cavity. In this sense, this model is similar to injection of live bacteria and, as such, results in similar symptoms and disease sequelae.…”

Section: The Sepsis Modelmentioning

confidence: 99%

Tissue oxygenation in sepsis; new insights from in vivo EPR

2004

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Nitric oxide (NO) is a key mediator in the maldistribution of oxygen by tissue and organ dysfunction observed in sepsis. Despite this, few techniques are capable of measuring these parameters directly in vivo. We describe here several techniques that have been developed by our group to address this directly by in vivo EPR in animal models of sepsis. Oxygen-sensitive materials can be implanted or administered and report on local tissue pO 2 . Spin trapping of NO can simultaneously report on tissue NO content. Repeat measures of these parameters can be made directly from a defined tissue site, allowing development of new models and experiments to study the defects in tissue and organ function seen in sepsis.

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“…failure and septic shock. (4)(5)(6)(7)(8) Treatments that reduce the generation or that prevent or reverse the effects of ROS have shown beneficial effects in a variety of models of endotoxic and septic shock. (9)(10)(11)(12)(13)(14)(15) Mitochondrial dysfunction has been strongly implicated in the pathogenesis of multiple organ dysfunction syndrome (MODS) and a wide variety of disease states.…”

Section: Introductionmentioning

confidence: 99%

Inibição da atividade da citrato sintase cerebral em um modelo animal de sepse

Scaini¹,

Rochi²,

Benedet³

et al. 2011

Rev. bras. ter. intensiva

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Objective: An extensive body of evidence from experimental studies indicates that sepsis is associated with increased reactive oxygen species production, depletion of antioxidants, and accumulation of markers of oxidative stress. Moreover, mitochondrial dysfunction has been implicated in the pathogenesis of multiple organ dysfunction syndrome (MODS). Citrate synthase is an enzyme localized in the mitochondrial matrix and an important component of the Krebs cycle; consequently, citrate synthase has been used as a quantitative enzyme marker for the presence of intact mitochondria. Thus, we investigated citrate synthase activity in the brains of rats submitted to a cecal ligation puncture model of sepsis.Methods: At several times points (3, 6, 12, 24 and 48 hours) after the cecal ligation puncture operation, six rats were killed by decapitation. Their brains were removed, and the hippocampus, striatum, cerebellum, cerebral cortex and prefrontal cortex were dissected and used

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Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Abstract: This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity

Cited by 171 publications

References 41 publications

Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation

Behavioral deficits in sepsis-surviving rats induced by cecal ligation and perforation

Tissue oxygenation in sepsis; new insights from in vivo EPR

Inibição da atividade da citrato sintase cerebral em um modelo animal de sepse

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