Oxidative phosphorylation in brain mitochondria of rats differing in their sensitivity to hypoxia

Luk’yanova, L. D.; Romanova, V. E.; Chernobaeva, G. N.

doi:10.1007/bf00841143

Cited by 9 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our experiments demonstrated that the initially similar efficiency of oxidative phosphorylation in the brain mitochondria of HR and LR rats is due to a higher velocity of phosphorylating respiration and strain of energy production in the LR animals [25]. This indicates that the processes of oxidative phosphorylation are less economic in them.…”

Section: Time Course Of Respiratory Dysfunction In Hypoxiamentioning

confidence: 91%

“…Due to them, the NADH-oxidase pathway of oxidation in the brain of LR rots is limited earlier and is more expressed than in HR animals [13][14][15][16][17]25,35,45]. Thus, although this route of oxidation is limiting at the early stages of hypoxia and determines the disorders of energy production in the brain of both HR and LR animals, the degree of its suppression in hypoxia is different.…”

Section: Time Course Of Respiratory Dysfunction In Hypoxiamentioning

confidence: 98%

See 1 more Smart Citation

Bioenergetic hypoxia: Definition, mechanisms, and methods of correction

Luk’yanova

1997

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

Bioenergetic hypoxia is defined as a phasic process starting at the substrate site of the respiratory chain with injury to the mitochondrial enzymatic complex I and involving, as oxygen insufficiency progresses, the terminal cytochrome site of the respiratory chain. Different stages of the process, determined experimentally, are described from a bioenergetic viewpoint. The development of bioenergetic hypoxia in tissues of aninlals with different resistance to hypoxia is analyzed. Modern concepts of the trigger mechanisms of bioenergetic hypoxia and approaches to correcting the function of the energy system at different stages of hypoxia are discussed. Key Words: bioenergetic hypoxia; energy metabolism; respiratory enzymatic complexes; individual sensitivity to hypoxia; adaptation; antihypoxantsHypoxia is a highly prevalent phenomenon, which develops both under conditions of oxygen deficiency in the environment and as a result of a variety of diseases involving the respiratory and cardiovascular systems and impairing blood transporting function. All such disorders lead to a decrease in oxygen delivery to tissues to a level which is insufficient for maintaining function, metabolism, and structure of the cell. Thus, hypoxia is an important problem of practical and theoretical medicine.This problem has been researched for more than a hundred years. In Russia it attracted the attention of I. M. Sechenov, V. V. Pashutin, P. M. Al'bitskii, and E. A. Kartashevskii. Later it became the main object of research for N. N. Sirotinin, A. M. Charnyi, I. R. Petrov, Z. I. Barbashova, M. N. Gaevskaya, and lnany others. Due to these investigations, stndy of hypoxic states became an important branch of fundamental and applied medicine, which still remains a priority trend for the Russian science. At Department of Bioenergetics, Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow present, a vast data bank on the mechanisms of hypoxia has been accumulated, permitting the creation of classifications of hypoxic states, development of prognostic criteria for assessing them, and analysis of the succession of disorders occurring under conditions Of oxygen deficiency.The intricate time course of this process and involvement of a wide spectrum of functional and metabolic systems regulating it at different levels of organization determine the multiplicity of the limiting sites and mechanisms underlying hypoxia. This l~act explains why, despite an almost lO0-year history of investigation, many pathogenetic aspects of hypoxia and many questions in antihypoxic defense are still not solved.The crucial factor leading to the development of h3rpoxic states is oxygen delivery from tile environment to the cell, where it participates ha reactions of aerobic energy production as the substrate of cytochrome oxidase (CCO) --the terminal exlzyme of the mitochondrial respiratory system. That is why oxygen deficiency under some conditions can limit or completely suppress aerobic energy production. The levels

show abstract

Section: Time Course Of Respiratory Dysfunction In Hypoxiamentioning

confidence: 91%

Section: Time Course Of Respiratory Dysfunction In Hypoxiamentioning

confidence: 98%

Bioenergetic hypoxia: Definition, mechanisms, and methods of correction

Luk’yanova

1997

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to LT, the rats were divided into groups of high resistance (HR, LT ≥ 10 min), middle resistance (MR, LT within 5-10 min), and low resistance (LR, LT within 1-5 min). These types of animals are known to demonstrate different functional and metabolic patterns, including differences in the CNS activity and neurohumoral regulation, stress-responsive systems, oxygen transport by the blood, and tissue respiration [27][28][29].…”

Section: Acute Hypobaric Hypoxiamentioning

confidence: 99%

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Граф

Trofimova

Ksenofontov

et al. 2020

Cells

View full text Add to dashboard Cite

Function of brain amino acids as neurotransmitters or their precursors implies changes in the amino acid levels and/or metabolism in response to physiological and environmental challenges. Modelling such challenges by pregnancy and/or hypoxia, we characterize the amino acid pool in the rat cerebellum, quantifying the levels and correlations of 15 amino acids and activity of 2-oxoglutarate dehydrogenase complex (OGDHC). The parameters are systemic indicators of metabolism because OGDHC limits the flux through mitochondrial TCA cycle, where amino acids are degraded and their precursors synthesized. Compared to non-pregnant state, pregnancy increases the cerebellar content of glutamate and tryptophan, decreasing interdependence between the quantified components of amino acid metabolism. In response to hypoxia, the dependence of cerebellar amino acid pool on OGDHC and the average levels of arginine, glutamate, lysine, methionine, serine, phenylalanine, and tryptophan increase in non-pregnant rats only. This is accompanied by a higher hypoxic resistance of the non-pregnant vs. pregnant rats, pointing to adaptive significance of the hypoxia-induced changes in the cerebellar amino acid metabolism. These adaptive mechanisms are not effective in the pregnancy-changed metabolic network. Thus, the cerebellar amino acid levels and OGDHC activity provide sensitive markers of the physiology-dependent organization of metabolic network and its stress adaptations.

show abstract

“…The concept was that in the brains of less tolerant rats the enzyme would become more rapidly saturated with the substrate (NADH) and would oxidize the substrate at a slower rate. This may underlie the lower baseline activity of the NADH-oxidase pathway of substrate oxidation in the brains of the less tolerant rats (Lukianova et al, 1991;Lukyanova, 1996). Kinetic peculiarities of the mitochondrial enzyme complex I also underlie a lower oxidative efficiency of NADH-cytochrome c oxidoreductase in the brain of less tolerant rats exposed to hypoxia.…”

Section: Individual Variabilitymentioning

confidence: 99%

Intermittent Hypoxia Research in the Former Soviet Union and the Commonwealth of Independent States: History and Review of the Concept and Selected Applications

Serebrovskaya

2002

High Altitude Medicine & Biology

123

View full text Add to dashboard Cite

This review aims to summarize the basic research in the field of intermittent hypoxia in the Soviet Union and the Commonwealth of Independent States (CIS) that scientists in other Western countries may not be familiar with, since Soviet scientists were essentially cut off from the global scientific community for about 60 years. In the 1930s the concept of repeated hypoxic training was developed and the following induction methods were utilized: repeated stays at high-mountain camps for several weeks, regular high altitude flights by plane, training in altitude chambers, and training by inhalation of low-oxygen-gas mixtures. To the present day, intermittent hypoxic training (IHT) has been used extensively for altitude preacclimatization; for the treatment of a variety of clinical disorders, including chronic lung diseases, bronchial asthma, hypertension, diabetes mellitus, Parkinson's disease, emotional disorders, and radiation toxicity, in prophylaxis of certain occupational diseases; and in sports. The basic mechanisms underlying the beneficial effects of IHT are mainly in three areas: regulation of respiration, free-radical production, and mitochondrial respiration. It was found that IHT induces increased ventilatory sensitivity to hypoxia, as well as other hypoxia-related physiological changes, such as increased hematopoiesis, alveolar ventilation and lung diffusion capacity, and alterations in the autonomic nervous system. Due to IHT, antioxidant defense mechanisms are stimulated, cellular membranes become more stable, Ca(2+) elimination from the cytoplasm is increased, and O(2) transport in tissues is improved. IHT induces changes within mitochondria, involving NAD-dependent metabolism, that increase the efficiency of oxygen utilization in ATP production. These effects are mediated partly by NO-dependent reactions. The marked individual variability both in animals and humans in the response to, and tolerance of, hypoxia is described. Studies from the Soviet Union and the CIS significantly contributed to the understanding of intermittent hypoxia and its possible beneficial effects and should stimulate further research in this direction in other countries.

show abstract

Oxidative phosphorylation in brain mitochondria of rats differing in their sensitivity to hypoxia

Cited by 9 publications

References 3 publications

Bioenergetic hypoxia: Definition, mechanisms, and methods of correction

Bioenergetic hypoxia: Definition, mechanisms, and methods of correction

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Intermittent Hypoxia Research in the Former Soviet Union and the Commonwealth of Independent States: History and Review of the Concept and Selected Applications

Contact Info

Product

Resources

About