Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields

Kormančíková, V.; Kováč, Ladislav; Vidová, Monika

doi:10.1016/0005-2728(69)90188-1

Cited by 40 publications

(17 citation statements)

References 34 publications

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“…From our results, with the wild-type strain box+, we can calculate, in g dry wt/mol: The values are not very high as has already been observcd [9]. It appears that with yeast, YATp varies widely depending on the substrate and experimental conditions; higher values of YATp (9 -14 g dry wtlmol ATP produced) were found in some conditions with lactate as substrate or glucose at very low concentration [9], and in anaerobiosis [15,16].…”

Section: Growth Yield Of' the Various Strainssupporting

confidence: 66%

“…At the non-permissive temperatures, 16 'C for box3-RC and 36 "C for box2-RT, these strains did not grow on ethanol since they are totally devoid of cytochrome b under these conditions ; they showed only a limited growth on galactose since a sugar can be fermented ( Y , = 24-27 g/mol, as that observed in anaerobic conditions with a sugar [9]). …”

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 78%

“…KormanCikovli et al [9] found a YEtoH of 70 g dry wt/mol at 30 'C and a YclC of 123 g dry wt/mol with a glucose concentration > 20 mM in aerobiosis, whereas Rogers and Stewart [15] measured a YGlc of 136 g/mol; no value has been given with galactose. These values were obtained with other strains of Saccharomyces cerevisiae; both Y G~, values are very similar to ours with galactose whereas there is some difference between the Y E t 0~ values.…”

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 99%

“…It appears that with yeast, YATp varies widely depending on the substrate and experimental conditions; higher values of YATp (9 -14 g dry wtlmol ATP produced) were found in some conditions with lactate as substrate or glucose at very low concentration [9], and in anaerobiosis [15,16]. In aerobiosis, divergences from our values or that mentioned by KormanCikova et al [9] appears in the estimation of YATP when authors try to express it as quantity of yeast formed per mole of ATP used for growth (taking into account the ATP necessary for the maintenance energy and the P/O ratio in vivo) [15,17,19]. In addition to maintenance, the energy unemployed for growth may also be used for the accumulation of ATP or other compound, or spoiled as heat.…”

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 99%

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Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Chevillotte-Brivet

Meunier-Lemesle

1980

European Journal of Biochemistry

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A wild-type strain of Saccharomyces cerevisiae and temperature-dependent revertants of isonuclear box mutants partially or completely devoid of cytochrome h-565, were used to study the role of this cytochrome in oxidative phosphorylation.At the mitochondrial level, the phosphorylating data and the succinate oxidase activity at three different temperatures (16 "C, 28 "C and 36 "C) were measured in these strains showing various cytochrome b-565 contents. It is concluded that this cytochrome b-565 is not in the main pathway of the electron transfer chain.At the optimum growth temperature (28"C), the measurements of the P/O ratio for the wild phenotype strains, with ethanol as substrate, led to the conclusions that two phosphorylation sites of the respiratory chain are functional in these strains.The growth yields for wild phenotype strains and revertants grown in vivo in complex media, with ethanol or galactose as the energy source (at 16 "C, 28 "C and 36 "C), were compared with the cytochrome b-565 content.The growth yields showed small variations if compared to the reference strain, when the cytochrome h-565 content was greatly diminished or absent.Thus the ATP production at site I1 is independent of the cytochrome h-565 content. Cytochrome b-565 does not play an essential role in oxidative phosphorylation.Two spectral forms of cytochrome b are present in the mitochondrial inner membrane; several hypotheses have been proposed to interpret results of redox potential, spectrophotometric and kinetic measurements but the question of the role of cytochrome h-565 in oxidative phosphorylation has not been solved.Yeast mitochondrial mutants which are affected in the synthesis of cytochrome b were isolated by Pajot et al. [I]. It was later shown that, in these strains, grown under certain conditions, the amount of cytochrome b-565 was extremely low [2], it seemed therefore possible to use these strains to investigate the Abbreviations and Symbols. Y., molar growth yield in g dry wt/ rnol substrate; EtOH, ethanol; rho", cytoplasmic petite, deleted of all mitochondrial DNA; P/O, mol ATP formed per electron pair transferred to oxygen; respiratory control ratio, ratio of respiratory rate in state 3 (+ADP) to that in state 4 (+ATP); CIPhzC(CN)Z, carbonylcyanide m-chlorophenylhydrazone. particular role of this cytochrome h species in oxidative phosphorylation.The investigations were carried out in vifro and in vivo. Zn vitro, the P/O ratio, respiratory control and respiration of mitochondria were correlated to the cytochrome b-562 and cytochrome b-565 contents. In vivo, the phosphorylation efficiencies of these isonuclear strains (which differ essentially in cytochrome h-565 concentrations) were compared by measuring the growth yields obtained by growing the cells under conditions of limited energy source. MATERIALS AND METHODS Yeast StrainsThe yeast strains used were described in the previous article [ 2 ] ; abbreviated names which refer to the

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Section: Growth Yield Of' the Various Strainssupporting

confidence: 66%

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 78%

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 99%

Section: Growth Yield Of' the Various Strainsmentioning

confidence: 99%

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Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Chevillotte-Brivet

Meunier-Lemesle

1980

European Journal of Biochemistry

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“…In the presence of excess UFA the final cell yield is proportional to glucose concentration and is 1.09 mg dry weight/mg glucose; this indicates a full utiliza- tion of the substrate by both fermentative and oxidative metabolism. 34 In the presence of the antibiotic chloramphenicol which inhibits the formation of functional mitochondria, ~5 the growth increment of 0.12 mg dry weight/mg glucose represents purely fermentative metabolism. 34 When the mutant is grown with limiting UFA the growth response curve shows three distinct phases.…”

Section: The Effect Of the Carbon Source On The Growth Yield And Fattmentioning

confidence: 99%

Biogenesis of mitochondria 19 the effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of

Proudlock

Haslam

Linnane

1971

J Bioenerg Biomembr

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Abstract1. The lipid composition of a mutant of Saccharomyces cerevisiae which cannot synthesize unsaturated fatty acid (UFA) can be extensively manipulated by growing the organism in the presence of added fatty acids.2. Growth of the mutant is supported by a wide range of unsaturated fatty acids including oleie, palmitoleic, petroselenic, l l-eicosaenoic, ricinoleic, araehidonic, clupanodonic, linoleie and linolenic acids; 9-and 10-hydroxystearic acids support growth less effectively, but erucic, nervonic, elaidic and saturated fatty acids (Cs:0-C20:0)* are ineffective. All the fatty acids which support growth are incorporated into cell lipids, apparently without further metabolism.3. The effects of altered lipid composition on the energy metabolism of yeast ceils were investigated. Cells containing less than approximately 20 % of their fatty acids as UFA cannot grow on non-fermentable substrates, and their growth on glucose is restricted to that which can be supported by fermentation alone.4. UFA-depleted cells contain mitochondria which are apparently normal in morphology, furthermore they have normal levels of cytochromes a + a3, b, cl and c and respire at normal rates. This suggests that the lesion in energy metabolism produced by UFAdepletion may be the loss of the ability of the mitochondria to couple respiration to phosphorylation.5. UFA-depleted cells incorporate added UFA into their cell lipids and subsequently regain the ability to grow on non-fermentable substrates, showing that the lesion in energy metabolism is fully reversible.

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Y_ATP value in Candida tropicalis grown on n‐alkanes, fatty acids, and acetate

Gallo¹,

Azoulay²

1975

Biotech & Bioengineering

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Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields

Cited by 40 publications

References 34 publications

Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Biogenesis of mitochondria 19 the effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of

Y_ATP value in Candida tropicalis grown on n‐alkanes, fatty acids, and acetate

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Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields

Cited by 40 publications

References 34 publications

Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Cytochrome b‐565 in Saccharomyces cerevisiae: Use of Mutants in the Cob‐Box Region of the Mitochondrial DNA to Study the Functional Role of this Spectral Species of Cytochrome b

Biogenesis of mitochondria 19 the effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of

YATP value in Candida tropicalis grown on n‐alkanes, fatty acids, and acetate

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Product

Resources

About

Y_ATP value in Candida tropicalis grown on n‐alkanes, fatty acids, and acetate