Oxidative state in the estrus cycle of the buffaloes: a preliminary study

Abstract

The oxidative status, the relationship between oxidizing agents (free radicals) and antioxidant system, is able to influence the correct performance of the oestrous cycle. The aim of the experimentation was to define a first range of physiological values of reactive oxygen species (ROS) and the biological antioxidant potential (BAP), found during the phases of the buffalo estrous cycle. In this study, blood samples were taken from 30 buffaloes in various phases of the estrous cycle (oestrus, dioestrus, anoestrus), on whose serum by photometer, determinations of BAP and ROS have been carried out. The highest ROS values were detected during oestrus and this is due to the primary role they play in determining ovulation. The highest levels of BAP have been ManuscriptClick here to access/download;Manuscript;manuscript buffalo.doc Click here to view linked References 2 found in dioestrus, when they perform protective action against oxidative damage in the ovaries and uterus.

Effect of the Season on Blood Changes of Oxidative Stress Index in the Italian Mediterranean Buffalo (Bubalis bubalis)

“…The study was performed on 30 healthy buffaloes, of which ten were in estrus, ten in diestrus, and ten in anestrus. The authors found the highest d-ROMs value and the lowest BAP level during the estrus phase, though the d-ROMs and BAP levels reported by the authors were lower than those observed in our study [33]. This finding may be related to the difference in the number of animals analyzed between the studies.…”

“…In addition, the reference values for these biomarkers have not yet been defined. Recently, a preliminary study reported the physiological range of d-ROMs and BAP levels during the different phases of the estrus cycle in buffaloes [ 33 ]. The study was performed on 30 healthy buffaloes, of which ten were in estrus, ten in diestrus, and ten in anestrus.…”

“…( D – F ) Boxplot of levels of d-ROMs, BAP and Osi during the hot and cold weather seasons in 2021 and 2022. The pink area shows references values for buffalo according to [ 33 ]. Dots represent outlier values.…”

Interrelationship between reproductive hormones and acute phase proteins during estrous cycle and pregnancy in Spanish purebred broodmares

“…Another point is that, the Hp concentration during estrous phase was significantly higher compared to diestrous and proestrous in non-synchronized estrous cycle in cows ( Samimi et al., 2020 ) , according to its higher production from the reproductive tract around the time of ovulation. What is more, behavioral stress during the ovulation, as the increased oxidative activity and released free radicals across estrous, could be other reasons for the Hp increases ( Sciorsci, Galgano, Mutinati & Rizzo, 2020 ).…”

Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares

“…Despite this, in non-synchronized estrous cycles in cows, haptoglobin (Hp) was significantly higher compared to diestrus and proestrus [ 37 ]. It seems that behavioral stress during ovulation in buffaloes, as increased oxidative activity and free radical release during estrus, could be other reasons for the increase of Hp [ 38 ]. However, Fe metabolism under these inflammatory response conditions was not evaluated in any of these studies.…”