Oxidative Stress and Aging: A Comparison between Vertebrates and Invertebrates

Rizvi, Syed Ibrahim; Pandey, Kanti Bhooshan

doi:10.1002/9781118148143.ch12

Cited by 1 publication

(1 citation statement)

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“…Under pathological conditions, this balance is often disrupted, which can lead to changes in biological function [3,5]. Oxidative stress, and eventually cell death, occurs when the concentration of free radicals exceeds the capacity of the intracellular antioxidant systems [6]; this plays a key role in the progression of a range of pathologies [4,7,8], including many aspects of cancer initiation and development [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Label-Free Confocal Raman Microspectroscopy for Monitoring Oxidative Stress In Vitro in Live Human Cancer Cells

2022

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Understanding the impact of free radicals and antioxidants in cell biology is vital; however, noninvasive nonperturbative imaging of oxidative stress remains a challenge. Here, we evaluated the ability of label-free Raman spectroscopy to monitor redox biochemical changes in antioxidant (N-acetyl-l-cysteine, NAC) and pro-oxidant (tert-butyl hydroperoxide, TBHP) environments. Cellular changes were compared to fluorescence microscopy using CellROX Orange as a marker of oxidative stress. We also investigated the influence of cell media with and without serum. Incubation of cells with NAC increased the Raman signal at 498 cm−1 from S-S disulphide stretching mode, one of the most important redox-related sensors. Exposure of cells to TBHP resulted in decreased Raman spectral signals from DNA/proteins and lipids (at 784, 1094, 1003, 1606, 1658 and 718, 1264, 1301, 1440, 1746 cm−1). Using partial least squares–discriminant analysis, we showed that Raman spectroscopy can achieve sensitivity up to 96.7%, 94.8% and 91.6% for control, NAC and TBHP conditions, respectively, with specificity of up to 93.5, 90.1% and 87.9%. Our results indicate that Raman spectroscopy can directly measure the effect of NAC antioxidants and accurately characterize the intracellular conditions associated with TBHP-induced oxidative stress, including lipid peroxidation and DNA damage.

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