Oxidative Stress and its connection to Jordanians' Red Blood Cell Storage

Abstract

Background: Red blood cells go through a number of metabolic changes throughout the course of a 0-35 day storage period at 1 to 6oC. The sodium/potassium pump is immobilized, resulting in a drop in intracellular potassium with an increase in cytoplasmic sodium levels, a fall in glucose levels, acidosis as a result of low pH levels, and other alterations known as "storage lesions" during the storage phase. Thus, the purpose of this study was to assess the oxidative condition of red blood cells during storage, as well as certain related characteristics. Study Design and Methods: The whole blood was drawn and placed in a citrate-phosphate-dextrose adenine anticoagulant (CPDA). Blood samples were collected in the appropriate tube for each test at various storage times (zero, 7, 14, 21, and 35 days). All statistical analyses were carried out using SPSS ver. 26.0 (SPSS Inc., Chicago, IL, USA). Results: All the studied parameters showed significant differences at 35 days of storage when compared to zero time. The pH level decreases over the storage period. After 35 days, the average extracellular K+ level rose from 4.23 mmol/L at zero time to 29.86mmol/L. There was a slight increase in mean corpuscular volume (MCV) as a result of RBC storage. Discussion: This study demonstrated that, when care was made to assess pH and potassium ion concentrations to increase blood safety, red blood cells preserved in current storage media that retained their properties had acceptable transfusion quality.