Oxidative Stress and Nonalcoholic Fatty Liver Disease in Hemodialysis Patients

Abstract

Introduction Nonalcoholic fatty liver disease (NAFLD) is becoming more common around the world and it may progress to cirrhosis and liver failure, increasing mortality risk. In hemodialysis (HD) patients, NAFLD may be a novel risk factor for their high cardiovascular mortality. Heightened oxidative stress is highly prevalent in HD patients. However, the relationship between oxidative stress and NAFLD in HD patients is not well defined. Methods We studied seventy-one stable nondiabetic HD patients. Nineteen patients had the diagnosis of NAFLD by ultrasonography. Blood levels of oxidative stress markers were measured in each patient, including thiobarbituric acid reactive substances (TBARS), free thiols, superoxide dismutase (SOD) activities, and glutathione peroxidase (GPx) activity. The copy numbers of mitochondrial DNA (mtDNA) in peripheral leukocytes were also determined. Demographic, biochemistry, and hemogram data were recorded. The two groups of patients were compared in order to determine the factors associated with NAFLD in HD patients. Findings Compared to those without NAFLD, nondiabetic HD patients with NAFLD had significantly higher mtDNA copy number and GPx levels. The two groups did not differ significantly in dialysis adequacy, hemoglobin, serum calcium, phosphorus, albumin, liver function tests, or lipid profiles. Regression analysis confirmed mtDNA copy numbers and GPx levels as two independent factors associated with NAFLD. Compared to those with polysulfone, patients dialyzed with cellulose membrane have significantly higher levels of TBARS. However, patients with or without NAFLD did not differ in their use of either dialysis membrane. Discussion Oxidative stress (represented by antioxidant defense, GPx) and mitochondrial DNA copy numbers are independently associated with fatty liver disease in nondiabetic HD patients. The diagnostic and therapeutic implications of this key observation warrant further exploration.

Association Between Leukocyte Mitochondrial DNA Copy Number and Non-alcoholic Fatty Liver Disease in a Chinese Population Is Mediated by 8-Oxo-2′-Deoxyguanosine

“…In the present study, participants with NAFLD had higher mtDNAcn, in agreement with previous studies ( 16 , 26 ). Malik et al ( 16 ) found elevated liver mtDNAcn in high-fat diet–induced mice.…”

The Systemic Redox Status Is Maintained in Non-Smoking Type 2 Diabetic Subjects Without Cardiovascular Disease: Association with Elevated Triglycerides and Large VLDL

“…In stable heart failure patients, higher levels of serum free thiols were associated with favourable disease outcomes [7]. In hemodialyis patients, free thiols tend to be decreased in the context of coinciding non-alcoholic fatty liver disease [10]. Furthermore, among renal transplant recipients higher free thiols concentrations were associated with a beneficial cardiovascular risk profile and a better patient and graft survival [11].…”

Lactobacillus paracasei Jlus66 extenuate oxidative stress and inflammation via regulation of intestinal flora in rats with non alcoholic fatty liver disease

“…According to our previous study, JLus66 effectively improved NAFLD by reducing of lipid accumulation (Ye et al, 2017). Now that OxS plays a key role in the course of NAFLD (Wu et al, 2018).…”

“…According to our previous study, JLus66 effectively improved NAFLD by reducing of lipid accumulation (Ye et al, ). Now that OxS plays a key role in the course of NAFLD (Wu et al, ). Therefore, in the present study, we aim to explore the protective effect of JLus66 on oxidative damages in HFD‐induced rats.…”