Oxidative stress, cell cycle arrest and differentiation contribute toward the antiproliferative action of BSO and calcitriol on Caco-2 cells

Liaudat, Ana Cecilia; Bohl, Luciana Paola; Talamoni, Nori G. Tolosa de; Maletto, Belkys A.; Pistoresi-Palencia, María C.; Picotto, Gabriela

doi:10.1097/cad.0000000000000109

Cited by 19 publications

(13 citation statements)

References 46 publications

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“…As the most biologically active metabolite of vitamin D 3 , the steroid hormone 1,25(OH) 2 D 3 is associated with multiple cellular functions, inducing cell cycle arrest, inhibition of cell division, and DNA breakage, thus preventing cell proliferation (Ramos-Martínez et al, 2013;Liaudat et al, 2014). This molecule has been reported to be an effective immunomodulator that acts on MCs to inhibit their proliferation, decreases albuminuria and inflammatory cell infiltration, and accelerates repair of glomerular injury.…”

Section: Discussionmentioning

confidence: 99%

Effect of 1,25(OH2D3 on the proliferation of human mesangial cells and their expression of Ki67

Zhao¹,

Zhang²,

Yang³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Previous studies have found that 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 or VD 3 ] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH) 2 D 3 on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and to establish its mechanism of action. Cultured human mesangial cells were randomly divided into the following four groups: normal control (N group; administered Dulbecco's modified Eagle's medium containing 5% fetal bovine serum), proliferation [ quantitative polymerase chain reaction were used to detect expression of Ki67 protein and mRNA, respectively. Compared to the N group, Ki67 levels were found to be higher in the EGF group but significantly lower in the VD 3 intervention group. Moreover, expression of Ki67 by cells in the EGF+VD 3 group was significantly lower than that of those in the EGF group. All of these differences were statistically significant (P < 0.05). In conclusion, 1,25(OH) 2 D 3 inhibited Ki67 expression and the proliferation of human mesangial cells; therefore, Ki67 may be regarded as a potent therapeutic target in mesangial proliferative glomerulonephritis.

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Section: Discussionmentioning

confidence: 99%

Effect of 1,25(OH2D3 on the proliferation of human mesangial cells and their expression of Ki67

Zhao¹,

Zhang²,

Yang³

et al. 2017

Genet. Mol. Res.

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“…SOD activity was determined as described previously [32]. Results were expressed as SOD units/mg protein (one SOD unit is the enzyme activity that inhibits 50% the NBT reduction).…”

Section: Superoxide Dismutase Activitymentioning

confidence: 99%

“…Malonaldehyde production was determined as previously [32]. Results were expressed in µmoles of MDA/mg protein.…”

Section: Malonaldehyde Productionmentioning

confidence: 99%

Soy genistein administered in soluble chitosan microcapsules maintains antioxidant activity and limits intestinal inflammation

Braber

Núñez

Bohl

et al. 2018

The Journal of Nutritional Biochemistry

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We used water-soluble Chitosan obtained by Maillard reaction with glucosamine to microencapsulate soy genistein (Ge) and preserve its biological activity for oral administration. Release of Ge was pH dependent with a super Case II mechanism at pH 1.2 and an anomalous transport with non-Fickian kinetics at pH 6.8. Microencapsulated Ge retained its antioxidant properties in vitro and its daily administration to mice attenuated clinical signs of acute colitis, limited inflammatory reaction and reduced oxidative stress and tissue injury as well. Remarkably, after feeding microencapsulated Ge the production of IL-10 in colonic tissue was restored to levels of untreated controls. According to statistical multivariate analysis, this cytokine was the parameter with the highest influence on the inflammatory/oxidative status. Microencapsulation of Ge with derivatized Chitosan becomes an interesting alternative to develop therapeutic approaches for oxidative inflammatory diseases; our findings suggest that the soy isoflavone could be incorporated into any functional food for application in intestinal inflammation.

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“…109 Thus, disruption of antioxidant systems is a potential target in several cancer cells that rely on antioxidants to compensate for elevated concentrations of ROS. 110,111 …”

Section: Ros-targeted Anticancer Agentsmentioning

confidence: 99%

Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability

2016

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Although the terms “excessive reactive oxygen species (ROS)” and “oxidative stress” are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activation of growth signals through thiol oxidation and may lead to DNA damage at elevated levels. In this review, we first discuss a conceptual framework for the interplay of ROS and antioxidants. This review then describes ROS signaling using FLT3-mediated growth signaling as an example. We then focus on ROS-mediated DNA damage. High concentrations of ROS result in various DNA lesions, including 8-oxo-7,8-dihydro-guanine, oxazolone, DNA–protein cross-links, and hydantoins, that have unique biological impacts. Here we delve into the biochemistry of nine well-characterized DNA lesions. Within each lesion, the types of repair mechanisms, the mutations induced, and their effects on transcription and replication are discussed. Finally, this review will discuss biochemically inspired implications for cancer therapy. Several teams have put forward designs to harness the excessive ROS and the burdened DNA repair systems of tumor cells for treating cancer. We discuss inhibition of the antioxidant system, the targeting of DNA repair, and ROS-activated prodrugs.

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Oxidative stress, cell cycle arrest and differentiation contribute toward the antiproliferative action of BSO and calcitriol on Caco-2 cells

Cited by 19 publications

References 46 publications

Effect of 1,25(OH<sub>2</sub>D<sub>3</sub> on the proliferation of human mesangial cells and their expression of Ki67

Effect of 1,25(OH<sub>2</sub>D<sub>3</sub> on the proliferation of human mesangial cells and their expression of Ki67

Soy genistein administered in soluble chitosan microcapsules maintains antioxidant activity and limits intestinal inflammation

Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability

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