Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Mamede, A.C.; Guerra, Sara; Laranjo, Mafalda; Santos, K.; Carvalho, Maria da Graça; Carvalheiro, Tiago; Moura, Paulo; Paiva, Artur; Abrantes, Ana Margarida; Maia, Cláudio J.; Botelho, Maria Filomena

doi:10.1007/s12253-016-0053-x

Cited by 18 publications

(10 citation statements)

References 47 publications

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“… Magatti et al (2012) and Bu et al (2017) have demonstrated that hAMSC and hAEC induce a cell cycle arrest in the G0/G1 phase in several cancer cell lines. Moreover, several research groups have shown that hAM and its derivatives promote apoptosis in cancer cells and also reduce the viability and affect the metabolism of cancer cells ( Jiao et al, 2012 ; Niknejad et al, 2013b , 2014 ; Mamede et al, 2014 , 2015 , 2016 ; Riedel et al, 2019 ). However, to the best of our knowledge, the effect of hAM on bladder cancer has not yet been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta

Jerman

Tratnjek

et al. 2020

Front. Bioeng. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Ramuta

Jerman

Tratnjek

et al. 2020

Front. Bioeng. Biotechnol.

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“…Studies in vivo revealed that hAMPEs were able to promote a complete tumor regression in mice inoculated with HepG2 but not with HuH-7 cells. These authors also demonstrated that hAMPEs negatively regulate protein and DNA content in all HCC lines 33 . They showed that these protein extracts have no effect on metabolic activity inhibition or in protein and DNA content on non-tumorigenic cell line 34 .…”

Section: Introductionmentioning

confidence: 80%

“…Mamede et al . concluded that hAMPE was capable of induce cell death through intrinsic or extrinsic pathways, depending on the treated hepatocarcinoma cell line 33 .…”

Section: Introductionmentioning

confidence: 99%

Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cells

Riedel

Pérez-Pérez

Carmona-Fernández

et al. 2019

Sci Rep

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The placental stem cells have called the focus of attention for their therapeutic potential to treat different diseases, including cancer. There is plenty evidence about the antiproliferative, antiangiogenic and proapoptotic properties of the amniotic membrane. Liver cancer is the fifth cause of cancer in the world, with a poor prognosis and survival. Alternative treatments to radio- or chemotherapy have been searched. In this work we aimed to study the antiproliferative properties of the human amniotic membrane conditioned medium (AM-CM) in hepatocarcinoma cells. In addition, we have analyzed the regulation of pro and antiOncomiRs expression involved in hepatocarcinoma physiology. We have determined by 3H-thymidine incorporation assay that AM-CM inhibits DNA synthesis in HepG2 cells after 72 h of treatment. AM-CM pure or diluted at 50% and 25% also diminished HepG2 and HuH-7 cells viability and cell number. Furthermore, AM-CM induced cell cycle arrest in G2/M. When proliferation mechanisms were analyzed we found that AM-CM reduced the expression of both Cyclin D1 mRNA and protein. Nuclear expression of Ki-67 was also reduced. We observed that this CM was able to promote the expression of p53 and p21 mRNA and proteins, leading to cell growth arrest. Moreover, AM-CM induced an increase in nuclear p21 localization, observed by immunofluorescence. As p53 levels were increased, Mdm-2 expression was downregulated. Interestingly, HepG2 and HuH-7 cells treatment with AM-CM during 24 and 72 h produced an upregulation of antiOncomiRs 15a and 210, and a downregulation of proOncomiRs 206 and 145. We provide new evidence about the promising novel applications of human amniotic membrane in liver cancer.

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“…Reduced glutathione (GSH) evaluation, an antioxidant defense, was performed with the fluorescent compound mercury orange. This compound reacts with GSH and this reaction product emits red fluorescence when excited at 488 nm [22,23]. A cell suspension of 10 6 cells was incubated for 15 min at room temperature in the dark with 40 mM of mercury orange (Sigma 83377).…”

Section: Reduced Glutathione Expressionmentioning

confidence: 99%

“…A cell suspension of 10 6 cells was incubated for 15 min at room temperature in the dark with 40 mM of mercury orange (Sigma 83377). After washing the cells with PBS, fluorescence was determined at 485/20 nm excitation and 590/35 nm emission [23].…”

Section: Reduced Glutathione Expressionmentioning

confidence: 99%

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

et al. 2020

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Tooth whitening procedures are increasing; however, side effects can occur, such as damage to pulp cells, by the whitening products. This study aims to assess the cellular effects promoted by a whitening product, namely, the oxidative stress fostered by the active agent hydrogen peroxide, with and without photoactivation. Additionally, if cellular recovery occurred, we intended to determine the time point where cells recover from the tooth whitening induced damage. Human fibroblasts were exposed to hydrogen peroxide, Zoom®, Zoom® + irradiation, and irradiation alone. The following analysis was performed: metabolic activity evaluation by the MTT assay; cell viability, mitochondrial membrane potential, peroxides production, superoxide radical production, and reduced glutathione expression by flow cytometry. We determined the IC50 value for all groups, and a dose-dependent cytotoxic effect was verified. At the times analyzed, hydrogen peroxide groups showed no metabolic activity recovery while a cell recovery was observed after 24 h (Zoom®) and 48 h (Zoom® + irradiation). Cell death was seen in hydrogen peroxide and Zoom® + irradiation groups, mainly by apoptosis, and the irradiation had a cytotoxic effect per se. This in vitro study supports that whitening products with moderate hydrogen peroxide (HP) concentration have a temporary effect on cells, allowing a cellular recovery.

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Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Cited by 18 publications

References 47 publications

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cells

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

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