Oxidative stress during phosphate deficiency in roots of bean plants (Phaseolus vulgaris L.)

Juszczuk, Izabela M.; Malusà, Eligio; Rychter, Anna M.

doi:10.1078/0176-1617-00541

Cited by 76 publications

(54 citation statements)

References 33 publications

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“…Hammond et al (2003) have shown that many genes that respond to P stress in Arabidopsis shoots also respond to other environmental challenges, including salinity, wounding, pathogen attack, anoxia, and other nutrient stresses. In bean, P stress results in the induction of oxidative responses, including increased lipid peroxidation, elevated peroxide levels, and increased catalase and peroxidase activity (Juszczuk et al, 2001). Our results confirm and extend this observation by showing that genes encoding proteins in several aspects of oxidative stress have enhanced expression in P-stressed roots.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

et al. 2007

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Phosphorus (P) is an essential element for plant growth. Crop production of common bean (Phaseolus vulgaris), the most important legume for human consumption, is often limited by low P in the soil. Functional genomics were used to investigate global gene expression and metabolic responses of bean plants grown under P-deficient and P-sufficient conditions. P-deficient plants showed enhanced root to shoot ratio accompanied by reduced leaf area and net photosynthesis rates. Transcript profiling was performed through hybridization of nylon filter arrays spotted with cDNAs of 2,212 unigenes from a P deficiency root cDNA library. A total of 126 genes, representing different functional categories, showed significant differential expression in response to P: 62% of these were induced in P-deficient roots. A set of 372 bean transcription factor (TF) genes, coding for proteins with Inter-Pro domains characteristic or diagnostic for TF, were identified from The Institute of Genomic Research/Dana Farber Cancer Institute Common Bean Gene Index. Using real-time reverse transcription-polymerase chain reaction analysis, 17 TF genes were differentially expressed in P-deficient roots; four TF genes, including MYB TFs, were induced. Nonbiased metabolite profiling was used to assess the degree to which changes in gene expression in P-deficient roots affect overall metabolism. Stress-related metabolites such as polyols accumulated in P-deficient roots as well as sugars, which are known to be essential for P stress gene induction. Candidate genes have been identified that may contribute to root adaptation to P deficiency and be useful for improvement of common bean.

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Section: Discussionmentioning

confidence: 99%

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

et al. 2007

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“…This role in stress mediation would explain the widespread occurrence of the alternative pathway in plants. Since the alternative pathway is able to maintain respiration free from the constraints imposed by low ADP and inorganic phosphate availability, it has also been postulated that it allows high decarboxylation fluxes during C4 and Crassulacean acid metabolism photosynthesis (Robinson et al, 1992;Chivasa et al, 1999) and respiration in plants growing under phosphorus limitation Juszczuk et al, 2001;Shane et al, 2004).…”

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confidence: 99%

Contribution of the Alternative Pathway to Respiration during Thermogenesis in Flowers of the Sacred Lotus

2006

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We report results from in vivo measurements, using oxygen isotope discrimination techniques, of fluxes through the alternative and cytochrome respiratory pathways in thermogenic plant tissue, the floral receptacle of the sacred lotus (Nelumbo nucifera). Fluxes through both pathways were measured in thermoregulating flowers undergoing varying degrees of thermogenesis in response to ambient temperature. Significant increases in alternative pathway flux were found in lotus receptacles with temperatures 16°C to 20°C above ambient, but not in those with lesser amounts of heating. Alternative pathway flux in the hottest receptacles was 75% of the total respiratory flux. In contrast, fluxes through the cytochrome pathway did not change significantly during thermogenesis. These data support the hypothesis that increased flux through the alternative pathway is responsible for heating in the lotus and that it is unlikely that uncoupling proteins, which would have produced increased fluxes through the cytochrome pathway, contribute significantly to heating in this tissue. Comparisons of actual flux, with capacity determined using inhibitors, suggested that the alternative pathway was operating at close to maximum capacity in heating tissues of lotus. However, in nonheating tissues the inhibitor data significantly overestimated the alternative pathway flux. This confirms that isotopic measurements are necessary for accurate determination of fluxes through the two pathways.

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“…During homogenization polyvinyl-pyrrolidone (PVP) (0.25 g) was added (modified by [40]). The homogenate was centrifugated twice for 10 min first at 10 000 x g and second at 20 000x g. For ascorbate peroxidase (APOX) assay the roots and green parts (1g) were homogenized according to [54], i.e. by adding 0.05 M phosphate buffer (pH 7.5) containing 1 mM EDTA, 1 mM sodium ascorbate, 1 mM DTT and 4.0% (w/v) polyvinyl-pyrrolidone (PVP), 1 mM EDTA (5 ml).…”

Section: Methodsmentioning

confidence: 99%

“…Root and washed shoot samples were divided into two parts. One was immediately stocked at −80°C until further biochemical analysis, while a second was cut into small pieces and dried in oven for 24 h [53], [54]. Mineralized samples of soil and plants were analyzed for Na, Ca, Fe, Zn, Cu, Mn, Cr, Ni, Cd, and Pb using inductively coupled plasma mass spectrometry ICP-MS (AGILENT 7500 CE; plasma ICP-MS spectrophotometer from Agilent Technologies Inc. (Palo Alto, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Enzymatic Antioxidant Responses of Plants in Saline Anthropogenic Environments

Kamiński¹,

Koim-Puchowska²,

Puchowski³

et al. 2012

Plant Science

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binding proteins such as calmodulins and calmodulin-binding proteins [2]. Reports showed close interaction between intracellular H2O2 and cytosolic calcium in response to biotic and abiotic stresses -increase in cytosolic calcium boosts the generation of H2O2. The protein calmodulin binds to activates plant catalases in the present of calcium. It indicated a dual function of Ca in regulating H2O2 homeostasis [30].Metals are involved in the direct or indirect generation of free radicals (FR) and reactive oxygen species in the following ways: 1. direct transfer of electron in the single electron reduction; 2. disturbance of metabolic pathways resulting in an increase in the rate of FR and ROS formation; 3. inactivation and down regulation of the enzymes of the antioxidative defence system, and 4. depletion of low molecular weight of antioxidants [33]. Microelements such as Fe, Zn, Cu and Mn fulfil various roles in the metabolism of plant organism and are necessary for the regularity of physiological processes, however the excess and deficiency of these elements leads also to disturbance of ionic homeostasis [34]. Fe, Mn, Cu, and Zn as transition metals have frequently unpaired electrons and they are, therefore, very good catalysts of oxygen reduction. In aqueous solutions at neutral pH, O2 and [48] have concluded that an oxidative stress could be involved in Cd toxicity, by either inducing oxygen free radical production, or by decreasing enzymatic and nonenzymatic antioxidants.The subject of numerous studies for at least the past four decades has been physiological responses of plants for salinity and heavy metals in the controlled laboratory conditions. However, many of these determinations are concentrated to one type of pollutant, whilst plants in the natural conditions are subjected to many stressful differentiated ecophysiological sources and factors. Therefore there still remains a need for research on the interdependencies of plants with multiple biotic and abiotic factors in their natural habitats, their adaptation mechanisms and responses. The aim of this paper was thus to investigate the enzymatic antioxidant mechanisms and responses in plants subjected to destabilization of chemical elements management in the natural conditions. We thus studied antioxidant enzymes SOD, CAT, and APOX, and the content of malondialdehyde (MDA) variations in different ecological groups of glycophytes Creeping thistle Cirisium arvense, Common nettle Urtica dioica, Yarrow Achillea millefolium, and Burdock Arctium lappa in various types of environments: salted and alkaline anthropogenic environments, agricultural environments Plant Science 38and also pollution free environments of the Pomeranian region of Poland. We also investigated the halophyte Common glasswort Salicornia europaea from only salted environments. Simultaneously, we examined the levels of chemical elements Na, Ca, Fe, Zn, Cu, Mn, Cd, and Pb in roots and green parts of plants as probably the factor generated reactive oxidant species and thus activated enzymat...

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Oxidative stress during phosphate deficiency in roots of bean plants (Phaseolus vulgaris L.)

Cited by 76 publications

References 33 publications

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

Contribution of the Alternative Pathway to Respiration during Thermogenesis in Flowers of the Sacred Lotus

Enzymatic Antioxidant Responses of Plants in Saline Anthropogenic Environments

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