Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

Pugliese, Michela; Biondi, Vito; Merola, Giordana; Landi, Alessandra; Passantino, Annamaria

doi:10.3390/antiox11020328

Cited by 6 publications

(7 citation statements)

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“…Oxidative stress is involved in the pathophysiology of numerous life-threatening diseases, and it is considered a potential pathogenic factor in the display of infectious and parasitic diseases in dogs [ 26 , 27 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…The intensity of the colored complex was inversely correlated with the total antioxidant capacity. A surplus of hypochlorous acid radicals present after the oxidative reaction indicated a reduction in the plasma barrier; conversely, a reduction in the values was directly correlated with an injury of the plasma barrier due to oxidation [ 24 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…The role of oxidative stress in dogs has been studied in patients affected by Leishmania infantum and Ehrlichia canis [ 26 , 27 ]. However, to the authors’ best knowledge, no study has evaluated oxidative stress induced by helminths in dogs; therefore, the aim of this study was to evaluate the concentration of reactive oxidative metabolites (R-OOHs), the antioxidant barrier (OXY), thiol groups of plasma compounds (SHp), and HGMB-1 in shelter dogs naturally infected with helminths.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

et al. 2022

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This study aimed to evaluate the concentration of reactive oxidative metabolites, the antioxidant barrier, thiol groups of plasma compounds, and high-mobility group box 1 in shelter dogs naturally infected with helminths. In addition, the correlation between clinical signs and oxidative stress was investigated. Sixty-six (41 male and 25 female) adult mixed-breed dogs housed in a shelter with the diagnosis of gastrointestinal nematodes (i.e., Ancylostoma spp., Uncinaria stenocephala, Toxocara canis, Toxascaris leonina, or Trichuris vulpis) were enrolled in Group 1 (G1) and twenty healthy adult dogs were included in Group 2 (G2), which served as the control. A clinical assessment was performed using a physician-based scoring system. Oxidative stress variables and high-mobility group box 1 were assessed and compared by the means of unpaired t-tests (p < 0.05). Spearman’s rank correlation was performed to calculate the correlation between oxidative stress variables, high-mobility group box 1, hematological parameters, and clinical signs. The results showed statistically significant values for reactive oxidative metabolites, thiol groups of plasma compounds, and high-mobility group box 1 in G1. Negative correlations between thiol groups and the number of red cells and hemoglobin were recorded. These preliminary results support the potential role of oxidative stress and HGMB-1 in the pathogenesis of gastrointestinal helminthiasis in dogs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

et al. 2022

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“…Ehrlichia canis, a gram-negative, obligate intracellular bacterium, is the cause of canine monocytic ehrlichiosis (CME), a tick-borne disease that affects dogs all over the world and is transmitted by ticks (Pugliese et al, 2022). Donetein made the initial discovery of the E. canis pathogen strain in Algeria in 1935.…”

Section: Ehrlichiosismentioning

confidence: 99%

Most common vector-borne diseases in dogs in Europe: a review

Jankauskaitė,

Zamokas,

Karvelienė

2023

biologija

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The wide variety of infectious and parasitic disorders known as vector-borne diseases are spread by blood-feeding parasites such as ticks, fleas, lice, and mosquitoes. Anaplasmosis, ehrlichiosis, babesiosis, and borreliosis are important for both animal welfare and their potential to spread to humans. In addition, several social and environmental factors, such as shifts in the planet temperature and ecosystems, an increase in animal and human mobility, and chemoresistance to insecticides and acaricides are constantly changing the epidemiological scenario of vector-borne diseases. When these arthropods feed on an infected animal, they pick up the disease-causing pathogens. When, subsequently, they feed on a healthy animal, they can transmit the pathogen to that animal through their saliva. Early detection and treatment of these diseases is critical to ensure the best possible outcomes for affected dogs. The aim of the article was to discuss the most common vector-borne diseases in dogs in Europe, looking at the prevalence of the diseases and the health risks for dogs. We attempted to summarise the latest literature on various aspects of the disease pathophysiology, epidemiology, advantages and disadvantages of diagnostic techniques, available treatments, and methods for prevention in dogs.

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“…For example, in human patients affected with Inflammatory Bowel Disease, markers of oxidative stress have been found to be consistently increased in the serum and have therefore been identified as a hallmark of the systemic effect of this disease on the patient’s health [ 22 ]. In dogs, markers of oxidative stress have been identified to be increased in numerous disease states, such as heart disease, systemic infectious diseases (e.g., Ehrlichia canis infection and leishmaniasis) and atopic dermatitis [ 23 , 24 , 25 ]. Moreover, oxidative stress has been found to contribute to the complex pathogenesis of IBD in dogs [ 26 ] and can be reversed back to homeostatic conditions after successful therapeutic management [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Probiotic Bacillus subtilis DE-CA9TM on Fecal Scores, Serum Oxidative Stress Markers and Fecal and Serum Metabolome in Healthy Dogs

Allenspach,

Sung,

Ceron

et al. 2023

Veterinary Sciences

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Background: There is increasing interest in the use of Bacillus species as probiotics since their spore-forming ability favors their survival in the acidic gastric environment over other probiotic species. The subsequent germination of B. subtilis to their vegetative form allows for their growth in the small intestine and may increase their beneficial effect on the host. B. subtilis strains have also previously been shown to have beneficial effects in humans and production animals, however, no reports are available so far on their use in companion animals. Study design: The goal of this study was therefore to investigate the daily administration of 1 × 109 cfu DE-CA9TM orally per day versus placebo on health parameters, fecal scores, fecal microbiome, fecal metabolomics, as well as serum metabolomics and oxidative stress markers in ten healthy Beagle dogs in a parallel, randomized, prospective, placebo-controlled design over a period of 45 days. Results: DE-CA9TM decreased the oxidative status compared to controls for advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS) and reactive oxygen metabolites (d-ROMS), suggesting an antioxidant effect of the treatment. Fecal metabolomics revealed a significant reduction in metabolites associated with tryptophan metabolism in the DE-CA9TM-treated group. DE-CA9TM also significantly decreased phenylalanine and homocysteine and increased homoserine and threonine levels. Amino acid metabolism was also affected in the serum metabolome, with increased levels of urea and cadaverine, and reductions in N-acetylornithine in DE-CA9TM compared to controls. Similarly, changes in essential amino acids were observed, with a significant increase in tryptophan and lysine levels and a decrease in homocysteine. An increase in serum guanine and deoxyuridine was also detected, with a decrease in beta-alanine in the animals that ingested DE-CA9TM. Conclusions: Data generated throughout this study suggest that the daily administration of 1 × 109 cfu of DE-CA9TM in healthy Beagle dogs is safe and does not affect markers of general health and fecal scores. Furthermore, DE-CA9TM administration had a potential positive effect on some serum markers of oxidative stress, and protein and lipid metabolism in serum and feces.

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Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

Cited by 6 publications

References 33 publications

Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

Oxidative Stress and High-Mobility Group Box 1 Assay in Dogs with Gastrointestinal Parasites

Most common vector-borne diseases in dogs in Europe: a review

Effect of the Probiotic Bacillus subtilis DE-CA9TM on Fecal Scores, Serum Oxidative Stress Markers and Fecal and Serum Metabolome in Healthy Dogs

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