Oxidative stress generated by diesel seawater contamination in the digestive gland of the Antarctic limpet Nacella concinna

Ansaldo, Martı́n; Najle, Roberto; Luquet, Carlos Marcelo

doi:10.1016/j.marenvres.2004.06.003

Cited by 36 publications

(14 citation statements)

References 23 publications

(20 reference statements)

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“…Interestingly, MDA levels decreased after exposure (24 h) to the highest PHE concentration, remaining low even after DEP. This pattern has already been reported with other aquatic species exposed to PAH mixtures (Arantes Felício et al, 2015;Nogueira et al, 2015) and on a time-dependent oxidative damage (Ansaldo et al, 2005;Lüchmann et al, 2011Lüchmann et al, , 2014. At the same time, the absence of increased MDA levels along with PHE bioaccumulation could indicate a low acute toxicity of this compound to N. nodosus.…”

Section: Discussionsupporting

confidence: 84%

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

et al. 2016

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Understanding the mechanism of phenanthrene (PHE) biotransformation and related cellular responses in bivalves can be an important tool to elucidate the risks of polycyclic aromatic hydrocarbons (PAHs) to aquatic organisms. In the present study it was analyzed the transcriptional levels of 13 biotransformation genes related to cytochrome P450 (CYP), glutathione S-transferase (GST), sulfotransferase (SULT), flavin-containing monooxygenase and fatty acid-binding proteins by qPCR in gill of scallops Nodipecten nodosus exposed for 24 or 96h to 50 or 200μgL(-1) PHE (equivalent to 0.28 and 1.12μM, respectively), followed by depuration in clean water for 96h (DEP). Likewise, it was quantified the activity of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH), GST and levels of lipid peroxidation. Increased transcriptional levels of CYP2UI-like, CYP2D20-like, CYP3A11-like, GSTomega-like, SULT1B1-like genes were detected in organisms exposed to PHE for 24 or 96h. In parallel, GR and GPX activities increased after 96h exposure to 200μgL(-1) PHE and G6PDH activity increased after 24h exposure to 50μgL(-1) PHE. This enhancement of antioxidant and phase I and II biotransformation systems may be related to the 2.7 and 12.5 fold increases in PHE bioaccumulation after 96h exposure to 50 and 200μgL(-1) PHE, respectively. Interestingly, DEP caused reestablishment of GPX and GR activity, as well as to the transcript levels of all upregulated biotransformation genes (except for SULT1B1-like). Bioaccumulated PHE levels decreased 2.5-2.9 fold after depuration, although some biochemical and molecular modifications were still present. Lipid peroxidation levels remained lower in animals exposed to 200μgL(-1) PHE for 24h and DEP. These data indicate that N. nodosus is able to induce an antioxidant and biotransformation-related response to PHE exposure, counteracting its toxicity, and DEP can be an effective protocol for bivalve depuration after PHE exposure.

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Section: Discussionsupporting

confidence: 84%

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

et al. 2016

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“…Similar results with increased SOD, CAT, GPx and GST activities were observed in the mussel Mytilus galloprovincialis subjected to contamination by an oil spill from a ship in Ibiza Harbour as suggested by Sureda et al (2011) and in the Antarctic limpet Nacella concinna in which increased activities of SOD, CAT and GST were associated with the presence of diesel hydrocarbons (Ansaldo et al 2005). In addition, other studies have shown a direct relationship between the high concentrations of PAHs in mussels' tissue and the activation of phase I and phase II biotransformation enzymes (Bebianno et al 2007;Cappello et al 2013).…”

Section: Discussionsupporting

confidence: 72%

“…To cope with pro-oxidative challenge, bivalves generally produce increased SOD activity as the first antioxidant enzymatic defence line (Ansaldo et al 2005;Lima et al 2007). SOD catalyses the dismutation of superoxide anion radicals (O 2…”

Section: Discussionmentioning

confidence: 99%

Environmental impact on the antioxidant responses inCorbicula fluminea(Bivalvia: Veneroida: Corbiculidae) from the Danube River

Vranković

2015

Italian Journal of Zoology

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The present study aims to assess the biological effects of different environmental pollution on the Asian clam Corbicula fluminea. Biomarkers indicative of oxidative stress (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx; glutathione reductase, GR; and the phase II biotransformation enzyme glutathione-S-transferase, GST) were measured in clams collected within the Serbian sector of the Danube River Basin at three locations: Višnjica, Zemun and Bela stena. Clams from Višnjica exhibited significantly higher SOD, CAT and GST activities than clams from the other two locations (p < 0.05), whereas GPx and GR activities do not have such a marked pattern. An alteration in the activity of SOD, CAT and GST reflects the presence of certain pro-oxidative chemicals that can lead to oxidative stress in clams at the Višnjica site. In conclusion, the noted antioxidant disparities among clam populations from different locations reflected physiological sensitivity of C. fluminea to environmental change, supporting its use as a sentinel organism in biomonitoring investigations.

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“…These results could be assigned to starvation, given that the last time polychaetes were fed was 48 h before the start of cadmium exposure. The effects of starvation on antioxidant enzymes were reported in another invertebrate, the Antarctic limpet ( Nacella concinna ) [41]. Those authors attributed decreased SOD and CAT activities to starvation in control limpets.…”

Section: Discussionmentioning

confidence: 99%

Short‐term responses to cadmium exposure in the estuarine polychaete Laeonereis acuta (Polychaeta, Nereididae): Subcellular distribution and oxidative stress generation

Sandrini

Regoli

Fattorini

et al. 2006

Enviro Toxic and Chemistry

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Some effects of cadmium exposure (100 microg/L for 4, 8, 12, and 24 h) on the estuarine polychaete Laeonereis acuta (Nereididae) were evaluated. This polychaete was able to accumulate cadmium in the body, with the metal stored mainly in the cytosolic fraction (>10 kDa). Activity of the antioxidant enzymes superoxide dismutase, glutathione S-transferase, and glutathione reductase (GR) as well as the total oxyradical scavenger capacity, the glutamate cysteine ligase catalytic subunit gene expression, and the metallothionein-like proteins content were not affected by cadmium at any exposure time tested. Catalase (CAT) activity, however, was significantly lower (p < 0.05) in worms treated with cadmium compared with that in controls after 8 h of exposure. At the same exposure time, lipid peroxide levels were increased (p < 0.05) in worms exposed to cadmium compared with those in control worms. Interestingly, CAT and GR activities decreased over time (p < 0.05) independent of cadmium treatment, which is a result that could be attributed to starvation. The effects caused by cadmium in the present study were observed only after 8 h of exposure, demonstrating that cadmium can generate oxidative stress.

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Oxidative stress generated by diesel seawater contamination in the digestive gland of the Antarctic limpet Nacella concinna

Cited by 36 publications

References 23 publications

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

Environmental impact on the antioxidant responses inCorbicula fluminea(Bivalvia: Veneroida: Corbiculidae) from the Danube River

Short‐term responses to cadmium exposure in the estuarine polychaete Laeonereis acuta (Polychaeta, Nereididae): Subcellular distribution and oxidative stress generation

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