Oxidative stress in dairy cows seropositives for Neospora caninum

Glombowsky, Patrícia; Bottari, Nathieli B.; Klauck, Vanderlei; Fávero, Juscivete F.; Soldá, Natan Marcos; Baldissera, Matheus D.; Perin, Gessica; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina; Stefani, Lenita M.; Silva, Aleksandro S. Da

doi:10.1016/j.cimid.2017.07.007

Cited by 15 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, oxidative damage-induced mitochondrial dysfunction caused by T. gondii would contribute to trophoblast apoptosis [ 51 ]. In the current study, accumulation of ROS was found in caprine EECs infected with intracellular N. caninum tachyzoites, consistent with in vivo findings in cows and gerbils [ 25 , 52 ]. Notably, significant decreases of ATP contents and mtDNA copy numbers and severe disruption of mitochondrion morphology were observed in N. caninum -infecting caprine EECs, suggesting that mitochondrial dysfunction of endothelial cells in the uterus would be associated with pathogenesis of N. caninum infection.…”

Section: Resultssupporting

confidence: 91%

“…However, due to poor understanding of the pathogenic mechanisms of N. caninum , there are no effective drugs and vaccines available currently against this disease. Neospora caninum infection has been reported to induce accumulation of ROS and reduction of ATP levels, resulting in oxidative damage in cows and gerbils [ 24 , 25 ]. Transcriptome analysis of cerebrovascular endothelial cells found that N. caninum infection induced increased expression of 21 mitochondrial genes that contributed to functions of Complex I, II, III, IV and V [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

et al. 2022

View full text Add to dashboard Cite

Background Infection of Neospora caninum, an important obligate intracellular protozoan parasite, causes reproductive dysfunctions (e.g. abortions) in ruminants (e.g. cattle, sheep and goats), leading to serious economic losses of livestock worldwide, but the pathogenic mechanisms of N. caninum are poorly understood. Mitochondrial dysfunction has been reported to be closely associated with pathogenesis of many infectious diseases. However, the effect of N. caninum infection on the mitochondrial function of hosts remains unclear. Methods The effects of N. caninum infection on mitochondrial dysfunction in caprine endometrial epithelial cells (EECs), including intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) contents, mitochondrial DNA (mtDNA) copy numbers and ultrastructure of mitochondria, were studied by using JC-1, DCFH-DA, ATP assay kits, quantitative real-time polymerase chain reaction (RT-qPCR) and transmission electron microscopy, respectively, and the regulatory roles of sirtuin 1 (SIRT1) on mitochondrial dysfunction, autophagy and N. caninum propagation in caprine EECs were investigated by using two drugs, namely resveratrol (an activator of SIRT1) and Ex 527 (an inhibitor of SIRT1). Results The current study found that N. caninum infection induced mitochondrial dysfunction of caprine EECs, including accumulation of intracellular ROS, significant reductions of MMP, ATP contents, mtDNA copy numbers and damaged ultrastructure of mitochondria. Downregulated expression of SIRT1 was also detected in caprine EECs infected with N. caninum. Treatments using resveratrol and Ex 527 to caprine EECs showed that dysregulation of SIRT1 significantly reversed mitochondrial dysfunction of cells caused by N. caninum infection. Furthermore, using resveratrol and Ex 527, SIRT1 expression was found to be negatively associated with autophagy induced by N. caninum infection in caprine EECs, and the intracellular propagation of N. caninum tachyzoites in caprine EECs was negatively affected by SIRT1 expression. Conclusions These results indicated that N. caninum infection induced mitochondrial dysfunction by downregulating SIRT1, and downregulation of SIRT1 promoted cell autophagy and intracellular proliferation of N. caninum tachyzoites in caprine EECs. The findings suggested a potential role of SIRT1 as a target to develop control strategies against N. caninum infection. Graphical abstract

show abstract

Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In line with the present results, it was recorded that the lipid oxidation biomarkers were significantly lower in the LIM breed compared to SIM and three other cattle breeds in China [51]. Enhanced lipid peroxidation levels have also been reported in infected [55][56][57], ketotic [58] or periparturient [59,60] dairy cows, indicating the significant one-dimensional effect that environmental and physiological conditions have on redox parameters, and the necessity of their documentation before any nutritional intervention. Therefore, the overall reduced endogenous antioxidant capacity of the CHA and SIM breeds compared to LIM might explain a possible failure in case a similar antioxidant treatment is implemented.…”

Section: Discussionsupporting

confidence: 88%

Redox Biomarker Baseline Levels in Cattle Tissues and Their Relationships with Meat Quality

et al. 2021

View full text Add to dashboard Cite

Cattle breeds or crossbreds with high productivity traits have been developed to meet a growing demand for food. When intensive farming practices are followed, animals face several challenges which can result in poor performance, compromised welfare and the reduced quality of their products. Our study aims to highlight the resting values of the physiological oxidative stress that three cattle breeds exhibit, and their potential relationship with meat quality. For this purpose, we determined the levels of five common redox biomarkers (glutathione (GSH), catalase (CAT), total antioxidant capacity (TAC), thiobarbituric reactive substances (TBARS) and protein carbonyls (CARBS)) in the tissues of three commonly used beef cattle breeds (Charolais (CHA), Limousin (LIM) and Simmental (SIM)) and their association with specific meat quality traits that depend on color, pH and texture. The results revealed that LIM cattle breed animals have elevated intrinsic antioxidant defense systems in comparison to CHA and SIM cattle breed animals. In addition, the meat quality parameters were associated with the redox biomarkers. We propose that the determination of specific antioxidant parameters in the blood might be used as potential biomarkers to predict meat quality. This would allow farmers to nutritionally intervene to improve the quality of their products.

show abstract

“…In our study, ADA levels from acutely infected bulls were higher than in the other groups of bulls (significant differences with non-infected and subclinically infected bulls). This increase in ADA activity has been described in the course of diseases that induce a cellular immune response, as for example in neosporosis [ 32 ] and toxoplasmosis [ 33 ]. Thus, increased ADA activity leads to decreased adenosine levels, which might regulate to the inflammatory process and tissue damage induced by B. besnoiti infection.…”

Section: Discussionmentioning

confidence: 95%

Changes in serum biomarkers of inflammation in bovine besnoitiosis

et al. 2021

View full text Add to dashboard Cite

Background Acute and chronic besnoitiosis in extensive natural-service herds can have relevant effects in the health of bulls and negative consequences in their productive performance. Recent progress has been made in order to elucidate the pathogenesis of this disease. In this context, the study of biomarkers of inflammation in serum would contribute to gaining knowledge about the physiopathology of bovine besnoitiosis. Serological biomarkers could help in early diagnosis and prognosis, as seropositive bulls may have mild or severe testicular lesions. Methods Herein, we have investigated the diagnostic and/or prognostic value of a panel of serum (serological) biomarkers related to inflammation, including total protein, globulin and albumin, haptoglobin (Hp), adenosine deaminase (ADA) paraoxonase-1 (PON-1) and acetylcholinesterase (AChE) in naturally and experimentally B. besnoiti-infected males classified according to different clinical phases of the disease (acute, chronic and subclinical besnoitiosis). Results Results showed a similar response pattern in these biomarkers for naturally and experimentally infected cattle, with a few relevant variations. Most significant changes occurred during the acute phase of infection, although significant changes in a few biomarkers were also observed during the chronic infection. Haptoglobin, albumin, PON-1 and ADA were identified as the biomarkers that showed changes of higher magnitude in the acute phase of the infection, whereas high total protein and globulin values were found in chronically infected cattle. We have described the changes of a panel of inflammatory biomarkers of acute and chronic bovine besnoitiosis. Conclusions In summary, several biomarkers with promising diagnostic value have been identified. The biomarkers associated with acute infection are related to previously reported molecular biomarkers in testicular parenchyma of infected bulls and could help in the diagnosis of early infections and complement results from specific immunoglobulin M (IgM) detection. Graphical abstract

show abstract

Oxidative stress in dairy cows seropositives for Neospora caninum

Cited by 15 publications

References 34 publications

Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

Redox Biomarker Baseline Levels in Cattle Tissues and Their Relationships with Meat Quality

Changes in serum biomarkers of inflammation in bovine besnoitiosis

Contact Info

Product

Resources

About