Oxidative stress of Microcystis aeruginosa induced by algicidal bacterium Stenotrophomonas sp. KT48

Lyu, Ping; Li, Huili; Zheng, Xiaoxu; Zhang, Hao; Yu, Qin; Xia, Bing; Wang, Dongsheng; Xu, Shengjun; Zhuang, Xuliang

doi:10.1007/s00253-022-11959-2

Cited by 15 publications

(4 citation statements)

References 57 publications

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“…These results showed that TTON treatment of S. aureus and E. coli increased lipid peroxidation, resulting in increased oxidative stress and free radical damage, leading to bacterial cell death 45 . ROS are produced by blocking electron transport in the respiratory chain on the damaged plasma membrane, and ROS levels increase when bacteria are damaged, which enhances lipid peroxidation and leads to cell damage 46,47 …”

Section: Resultsmentioning

confidence: 99%

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Preparation of tea tree oil nanoemulsion: Characterisation, antibacterial mechanism and evaluation of apoptosis

Han

Wang

Zhuansun

et al. 2023

Flavour & Fragrance J

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Tea tree oil (TTO) is a natural plant essential oil that has strong bactericidal properties and low toxicity. However, owing to its irritability, volatility, instability under light/oxygen and hydrophobicity, the application of TTO in pharmaceutical production technology and the food industry is limited. To overcome these problems, TTO nanoemulsions (TTONs) were prepared using a high-speed shearing method. The mean droplets size of TTON was measured. TTON droplets were observed using confocal laser scanning microscopy and analysed using a Turbiscan scanning spectrum. The most suitable nanoemulsion formula was obtained as follows: TTO content, 10% (w/w); Tw-40 content, 8% (w/w); deionised water content, 82% (w/w); shearing time, 5 minutes. The antibacterial effect of TTON was studied, and it was found that its antibacterial activity against Escherichia coli was more potent than that against Staphylococcus aureus. The antibacterial mechanism of TTON was also investigated by observing bacterial morphology using scanning electron microscopy and testing the levels of bacterial reactive oxygen species. The effect of TTON on L-02 cells was evaluated. The results showed that the cell survival rates of L-02 cells treated with TTON at 0.05-0.25 μg/μL were all >90%. At each concentration, the total apoptosis rate of L-02 cells was <10%. These results indicated the high biocompatibility and biological safety of TTON. Therefore, the preparation of TTO into a nanoemulsion can improve its bioavailability, which has positive implications for further applications of TTO.

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Section: Resultsmentioning

confidence: 99%

“…45 ROS are produced by blocking electron transport in the respiratory chain on the damaged plasma membrane, and ROS levels increase when bacteria are damaged, which enhances lipid peroxidation and leads to cell damage. 46,47…”

Section: Antibacterial Mechanism Of Ttonmentioning

confidence: 99%

Preparation of tea tree oil nanoemulsion: Characterisation, antibacterial mechanism and evaluation of apoptosis

Han

Wang

Zhuansun

et al. 2023

Flavour & Fragrance J

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“…Still, this did not immediately result in a reduction in the amount of chlorophyll‐ a at 36 h. The fluorescence value in Figure 2c correlated with chlorophyll‐ a content, which had already been synthesized in the cells (Figures 3e and 2c). The result of down‐regulated photosynthesis genes (e.g., psa B, psb D, and rbc L) is a lack of electron acceptors, less reductive potential production for carbon assimilation, and the production of superoxide anions (O 2 − ) and hydrogen peroxide (H 2 O 2 ) in M. aeruginosa exposed to streptomycin (Lyu et al., 2022; Qian et al., 2012; Zhang et al., 2014).…”

Section: Resultsmentioning

confidence: 99%

Antagonistic actions of Paucibacter aquatileB51 and its lasso peptide paucinodin toward cyanobacterial bloom‐forming Microcystis aeruginosaPCC7806

Cha,

Kim,

Park

et al. 2023

Journal of Phycology

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Superior antagonistic activity against axenic Microcystis aeruginosa PCC7806 was observed with Paucibacter sp. B51 isolated from cyanobacterial bloom samples among 43 tested freshwater bacterial species. Complete genome sequencing, analyzing average nucleotide identity and digital DNA–DNA hybridization, designated the B51 strain as Paucibacter aquatile. Electron and fluorescence microscopic image analyses revealed the presence of the B51 strain in the vicinity of M. aeruginosa cells, which might provoke direct inhibition of the photosynthetic activity of the PCC7806 cells, leading to perturbation of cellular metabolisms and consequent cell death. Our speculation was supported by the findings that growth failure of the PCC7806 cells led to low pH conditions with fewer chlorophylls and down‐regulation of photosystem genes (e.g., psbD and psaB) during their 48‐h co‐culture condition. Interestingly, the concentrated ethyl acetate extracts obtained from B51‐grown supernatant exhibited a growth‐inhibitory effect on PCC7806. The physical separation of both strains by a filter system led to no inhibitory activity of the B51 cells, suggesting that contact‐mediated anti‐cyanobacterial compounds might also be responsible for hampering the growth of the PCC7806 cells. Bioinformatic tools identified 12 gene clusters that possibly produce secondary metabolites, including a class II lasso peptide in the B51 genome. Further chemical analysis demonstrated anti‐cyanobacterial activity from fractionated samples having a rubrivinodin‐like lasso peptide, named paucinodin. Taken together, both contact‐mediated inhibition of photosynthesis and the lasso peptide secretion of the B51 strain are responsible for the anti‐cyanobacterial activity of P. aquatile B51.

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“…In addition to calculating the algicidal rate, the impact of algicidal bacteria can also be seen through a series of physiological and biochemical changes in algal cells. Contemporary studies addressing the characterization of algal cell damage induced with stress-related alterations have focused on three primary echelons: the cellular level [20], enzymatic level [21], and photosynthetic level [22]. At the cellular level, alterations manifest as changes in chlorophyll content and cell numbers; for instance, toxic chemicals can induce a decrease in the chlorophyll content of K. mikimotoi by detrimentally affecting the cell structure and impeding chlorophyll synthesis [23,24].…”

Section: Introductionmentioning

confidence: 99%

Pilot-Scale Fermentation of Pseudoalteromonas sp. Strain FDHY-MZ2: An Effective Strategy for Increasing Algicidal Activity

Zhong,

Zheng,

Shi

et al. 2023

Biology

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The role of microorganisms in effectively terminating harmful algal blooms (HABs) is crucial for maintaining environmental stability. Recent studies have placed increased emphasis on bio-agents capable of inhibiting HABs. The bacterium Pseudoalteromonas sp. strain FDHY-MZ2 has exhibited impressive algicidal abilities against Karenia mikimotoi, a notorious global HAB-forming species. To augment this capability, cultures were progressively scaled from shake flask conditions to small-scale (5 L) and pilot-scale (50 L) fermentation. By employing a specifically tailored culture medium (2216E basal medium with 1.5% soluble starch and 0.5% peptone), under precise conditions (66 h, 20 °C, 450 rpm, 30 L/min ventilation, 3% seeding, and constant starch flow), a notable increase in algicidal bacterial biomass was observed; the bacterial dosage required to entirely wipe out K. mikimotoi within a day decreased from 1% to 0.025%. Compared to an unoptimized shake flask group, the optimized fermentation culture caused significant reductions in algal chlorophyll and protein levels (21.85% and 78.3%, respectively). Co-culturing induced increases in algal malondialdehyde and H2O2 by 5.98 and 5.38 times, respectively, leading to further disruption of algal photosynthesis. This study underscores the unexplored potential of systematically utilized microbial agents in mitigating HABs, providing a pathway for their wider application.

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Oxidative stress of Microcystis aeruginosa induced by algicidal bacterium Stenotrophomonas sp. KT48

Cited by 15 publications

References 57 publications

Preparation of tea tree oil nanoemulsion: Characterisation, antibacterial mechanism and evaluation of apoptosis

Preparation of tea tree oil nanoemulsion: Characterisation, antibacterial mechanism and evaluation of apoptosis

Antagonistic actions of Paucibacter aquatileB51 and its lasso peptide paucinodin toward cyanobacterial bloom‐forming Microcystis aeruginosaPCC7806

Pilot-Scale Fermentation of Pseudoalteromonas sp. Strain FDHY-MZ2: An Effective Strategy for Increasing Algicidal Activity

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