OXIDIZED LIPIDS DID NOT REDUCE LIFESPAN IN THE FRUIT FLY, Drosophila melanogaster

Abstract

Aging is often associated with accumulation of oxidative damage in proteins and lipids. However, some studies do not support this view, raising the question of whether high levels of oxidative damage are associated with lifespan. In the current investigation, Drosophila melanogaster flies were kept on diets with 2 or 10% of either glucose or fructose. The lifespan, fecundity, and feeding as well as amounts of protein carbonyls (PC) and lipid peroxides (LOOH), activities of superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST), and glutathione reductase activity of thioredoxin reductase (TrxR) were measured in "young" (10-day old) and "aged" (50-day old) flies. Flies maintained on diets with 10% carbohydrate lived longer than those on the 2% diets. However, neither lifespan nor fecundity was affected by the type of carbohydrate. The amount of PC was unaffected by diet and age, whereas flies fed on diets with 10% carbohydrate had about fivefold higher amounts of LOOH compared to flies maintained on the 2% carbohydrate diets. Catalase activity was significantly lower in flies fed on diets with 10% carbohydrates compared to flies on 2% carbohydrate diets. The activities of SOD, GST, and TrxR were not affected by the diet or age of the flies. The higher levels of LOOH in flies maintained on 10% carbohydrate did not reduce their lifespan, from which we infer that oxidative damage to only one class of biomolecules, particularly lipids, is not sufficient to influence lifespan.

Insulin-Like Peptides Regulate Feeding Preference and Metabolism in Drosophila

“…As in previous studies, we have found that fruit flies consume more food on diets with low rather than higher percentages of carbohydrates, indicative of compensatory feeding for carbohydrates (Lushchak et al, 2011, 2012, 2014, 2016; Rovenko et al, 2015a). DILPs are known to be connected with signaling pathways that control appetite (Wu et al, 2005; Itskov and Ribeiro, 2013; Nässel et al, 2013; Luo et al, 2014; Lushchak et al, 2015), and diminution of insulin signaling leads to increased consumption of unpalatable food by fruit flies or their larvae (Wu et al, 2005; Luo et al, 2014).…”

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

“…We also found that aged flies had higher levels of PCO when compared to young ones. Our current result contradicted with Lushchak et al ().The magnitude of PCO formation may not depend on aging process in D. melanogaster. On the other hand, DT and AGEs levels were affected from gender.…”

“…On the other hand, we found that LHP levels were not affected to gender and age. Our results represents a significant similarity with Lushchak et al (Lushchak et al, ). It is well known that oxidative damage is seen in the aging period and increased oxidative stress may lead to a decreased life span.…”

“…Cellular macromolecules such as proteins, lipids, carbohydrates, and DNA are vulnerable to free radicals and free radical‐derived products (Kirkwood, ; Ray, Huang, & Tsuji, ; Sinha, Ghosh, Swain, Giridharan, & Raghunath, ). The excessive production of the free radicals and the diminished activity of the antioxidant enzymes and molecules lead to macromolecular cellular damage (Lushchak, Gospodaryov, Yurkevych, & Storey, ). These damages represent their existence via biomarkers.…”

“…The excessive production of the free radicals and the diminished activity of the antioxidant enzymes and molecules lead to macromolecular cellular damage (Lushchak, Gospodaryov, Yurkevych, & Storey, 2016). These damages represent their existence via biomarkers.…”