Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

Batt, P. A.; Gardner, David K.; Cameron, A. W. N.

doi:10.1071/rd9910601

Cited by 142 publications

(61 citation statements)

References 24 publications

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“…Oxygen at a concentration of 20% (atmospheric) has been shown to adversely affect embryonic development compared with embryos cultured at 5% (Quinn & Harlow 1978, Batt et al 1991, Gardner & Lane 1996. These data further support the notion that it is not appropriate to culture the preimplantation mammalian embryo in the presence of atmospheric oxygen.…”

Section: Discussionsupporting

confidence: 75%

A proteomic analysis of mammalian preimplantation embryonic development

Katz‐Jaffe¹,

Linck²,

Schoolcraft³

et al. 2005

Reproduction

118

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Genetic studies on the mammalian preimplantation embryo are providing a wealth of information regarding gene expression. However, changes in the transcriptome do not always reflect cellular function or the complexity and diversity of the mammalian proteome with post-translational modifications or protein-protein interactions. To elucidate embryonic cellular function, a detailed understanding at the protein level is necessary. The aim of this study was to generate protein profiles of mammalian embryos throughout development, and to investigate the effects of oxygen concentration on the embryonic proteome. A protocol was developed to analyse small groups of embryos (n 5 5) by time-of-flight mass spectrometry. F1 mice zygotes were cultured in G1/G2 sequential media with recombinant albumin (2.5 mg/ml) in 6% CO 2 and O 2 concentrations of either 5% or 20%. In vivo-developed embryos were flushed from the reproductive tract (day 4). Protein profiles were generated for all embryonic samples and statistical analysis revealed 32 potential proteins/biomarkers with significant changes (P < 0.05). Embryos generated under 5% O 2 more closely resembled in vivo-developed embryos. Under 20% O 2 conditions, embryos showed down-regulation of 10 proteins/biomarkers (masses between 4 to 20 kDa) (P < 0.05) confirming the pathological effects of oxygen during embryonic development. These data demonstrate for the first time the complexity of the mammalian preimplantation proteome. The unique protein profiles of in vivo-developed embryos and a panel of selected biomarkers represent optimal cellular function, against which comparisons can be made to facilitate improvements in mammalian assisted reproduction techniques procedures.

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Section: Discussionsupporting

confidence: 75%

A proteomic analysis of mammalian preimplantation embryonic development

Katz‐Jaffe¹,

Linck²,

Schoolcraft³

et al. 2005

Reproduction

118

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“…Toxic effects of atmospheric oxygen concentration under an ordinary culture conditions and beneficial effect of lower O 2 concentrations (5-7%) on embryo development have been reported in mouse [1,[30][31][32][33][34], hamster [2,35], rat [3], pig [4,[36][37][38], sheep [5,39,40], goat [6], cow [5,[41][42][43][44] (Fig. 2) and human [7,[45][46][47].…”

Section: Effects Of Oxidative Stress On Development Of Preimplantatiomentioning

confidence: 99%

“…20%) [1][2][3][4][5][6][7]. Detrimental effect of O 2 concentration has correlated with the generation of ROS [8,9].…”

mentioning

confidence: 99%

Oxidative Stress and Redox Regulation on <i>In Vitro</i> Development of Mammalian Embryos

2012

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Abstract. Many factors affect development of mammalian preimplantation embryos in vitro. It is well known that in vitro development of bovine embryos is highly affected by culture condition including energy source, growth factors, pH or gas environment. Many efforts have been made towards the suitable environments which can successfully support embryo development in vitro. For a rapid growth and differentiation, embryo requires energy by utilizing ATP, NADPH with oxygen molecules. These energy substrates are produced from the electron transport chain in the mitochondria. In addition to energy production, reactive oxygen species (ROS) are also generated as by-product of such energy production system. ROS production is sensitively controlled by the balance of oxidizing and reducing status and affected by several antioxidant enzymes such as superoxide dismutase (SOD), Catalase, glutathione peroxidase (GPx) or low molecular weight thiols such as glutathione (GSH). Imbalance of oxidation and reduction causes production of excess ROS, which causes the developmental arrest, physical DNA damage, apoptosis induction or lipid peroxidation. Environmental oxygen condition during embryo culture also highly affects embryo development as well as intracellular redox balance. Several studies have revealed that regulation of intra-and extra-cellular reducing environment by reducing excess ROS by using antioxidants, reducing oxygen concentration are effective for improving embryo development. Also, recent studies have demonstrated the difference in gene expression affected by oxidative stress. This review briefly summarizes the effects of ROS and the role of redox balance on preimplantation embryos for improving the efficiency of in vitro production of mammalian embryos. Key words: Embryo development, Glutathione, Intracellular redox state, Reactive oxygen species (J. Reprod. Dev. 58: 1-9, 2012) H andling of oocytes and embryos in vitro is an effective technique for obtaining offspring by embryo transfer, investigating the mechanisms of early embryonic development and differentiation, gene functions by transgenic approach and research of somatic cell clones in mammals. To improve the pregnancy rates in in vitro produced (IVP) embryos, it is important to evaluate and improve the quality of embryos suitable for transfer. Therefore, increasing the number of good quality embryos as well as selection of superior quality embryos is one of the most practical and clinically useful approaches in embryo transfer of mammalian preimplantation embryos. It is well known that in vitro development of bovine embryos is highly affected by culture condition. Therefore, much effort has been made towards the effective culture methods that support embryo development in vitro. The detrimental effect of oxidative stress caused by a high oxygen concentration under culture condition on embryo development has been studied with the comparison of oxygen concentration in in vivo condition.It was found that embryo development is promoted under a low O 2 concent...

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“…In contrast, human serum contains a heterogeneous mixture of many proteins and other factors. It has been demonstrated that human serum enhances blastocyst formation in cow [19], goat [1], sheep [9] and pig [30] embryos in culture, while on the other hand it inhibits embryo development in mice [21]. In feline embryos, it appears that unidentified factors in human serum stimulate blastocoel cavity formation without effect on cell division.…”

Section: Discussionmentioning

confidence: 99%

Development of in vitro Fertilized Feline Embryos in a Modified Earle's Balanced Salt Solution: Influence of Protein Supplements and Culture Dishes on Fertilization Success and Blastocyst Formation.

Kanda

Miyazaki

Kanda

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of protein supplements and culture dish type on in vitro fertilization (IVF) and embryo development in culture were examined in the domestic cat. In Experiment I, follicular oocytes were fertilized and cultured in either 1) modified Earle's balanced salt solution, designated MK-1, supplemented with one of the following: 10% human serum (HS), 10% FCS or 0.4% BSA, or 2) Medium 199 (M-199) supplemented with 10% FCS. Fertilization rates were lower (P<0.01) in MK-1 + BSA (74.4%), MK-1 + FCS (56.1%), and M-199 + FCS (51.4%) than in MK-1 + HS (94.7%). A greater (P<0.01) percentage of blastocysts was obtained in MK-1 + HS (50.0%) than in other treatment groups (range, 4.3-17.2%). In Experiment II, the effect of dish type (tissue culture dish, TCD, versus suspension culture dish, SCD) on embryo development was evaluated in MK-1 supplemented with either HS or BSA. Significantly higher proportions of IVF-derived embryos developed to blastocysts at 120 and 144 hr post-insemination, respectively, when cultured in HS/SCD (47.2 and 71.7%) than in BSA/SCD (11.4 and 27.3%) or BSA/TCD (10.4 and 25.0%). At 120 hr post-insemination, there was a lower (P<0.01) percentage of blastocysts in HS/TCD (22.2%) than in HS/SCD. In Experiment III, six embryos per cat were transferred to the uterine horns of 17 recipients at 144 hr after hCG treatment. Five of 7 recipients which received late morulae cultured in MK-1 + BSA (SCD) for 120 hr became pregnant (71.4%). Eight of 10 recipients which received early blastocysts cultured in MK-1 + HS (SCD) for 120 hr became pregnant (80.0%). We conclude that MK-1 containing HS is highly beneficial for overcoming the in vitro developmental block of IVF-derived feline embryos and increasing the success rate of IVF/ET. -KEY WORDS: developmental block, feline (domestic), human serum, in vitro culture, IVF-ET.

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Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

Cited by 142 publications

References 24 publications

A proteomic analysis of mammalian preimplantation embryonic development

A proteomic analysis of mammalian preimplantation embryonic development

Oxidative Stress and Redox Regulation on <i>In Vitro</i> Development of Mammalian Embryos

Development of in vitro Fertilized Feline Embryos in a Modified Earle's Balanced Salt Solution: Influence of Protein Supplements and Culture Dishes on Fertilization Success and Blastocyst Formation.

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