Oxygen consumption in T‐47D cells immobilized in alginate

Abstract

The experimental set-up presented here may be varied in multiple ways by changing the cell-gel construct 3-D microenvironment, easily allowing investigation of a variety of factors on cell respiration.

Microenvironment‐dependent respiration of T‐47D cells cultured in alginate biostructures

“…The increased number of data points, however, had minimal impact on R 2 values of linear regressions. As described previously, there was no significant fall in viability during the first 24 h in biostructures cultured under similar conditions , a finding we have also confirmed for several matrices and other types of cells (unpublished data).…”

“…N 0 denotes the number of cells originally added to the gel as estimated by counting samples of cell suspension in five plots of two separate Bürker chambers, and V gel is the volume of hydrogel used. This procedure was described in greater detail in our previous work .…”

“…. In a previous work, our group presented a model that allows characterization of oxygen metabolism of cells entrapped in alginate 3D structures . Here, we have used this model to determine how matrix composition influences OCRs of cells immobilized in different matrices, with known physical characteristics, in the presence and absence of foetal bovine serum (FBS).…”

Rheological characterization of an injectable alginate gel system

“…We have here studied several parameters influencing gelling kinetics and elasticity in particular. We have earlier found that the gel system allows conveniently entrapping cells in a 3D environment while preserving a high degree of viability, and that it was useful in a model system for the determination of cell respiration in this respect [ 34 , 35 ]. It has also been useful in constructs for cartilage regeneration and a formulation is currently undergoing clinical trials as a treatment for dilated cardiomyopathy [ 36 - 38 ].…”

Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds

“…This indicates that the open-pore scaffold structure was sufficiently preserved by the alginate hydrogel and the EMD. Larsen et al 24 recently demonstrated the impact of the distance between cells immobilized in alginate to the cell medium interface in terms of oxygen diffusion. The findings from this study suggested that the distance between the immobilized cells to the cell medium interface was small enough to maintain viability.…”