Oxygen Deficiency Responsive Gene Expression in Chlamydomonas reinhardtii through a Copper-Sensing Signal Transduction Pathway

Quinn, J. M.

doi:10.1104/pp.128.2.463

Cited by 36 publications

(82 citation statements)

References 18 publications

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“…As reported previously (26), after ϳ40 min in the dark and anoxia, 7 the fraction of oxidizable P 700 , estimated by the signal after being induced by the saturating light pulse (32), reached 100% thus showing that the acceptor side limitation is spontaneously alleviated during this incubation. In turn, the lightinduced transmembrane electric field reached after 10 s of illumination hardly changed with the duration of incubation in anoxia.…”

Section: Resultssupporting

confidence: 85%

“…Notably, the deviation was observed at all light intensities tested here. The deviation decreased as the cells were incubated for longer times in the dark and anoxia, and the data fell on the diagonal after 40 min of incubation indicating a 7 We note here that the duration of the incubation in the dark and anoxia required to reach the metabolic status corresponding to those described in the text and the figures should be taken as indicative and may vary between batches. The data shown in Fig.…”

Section: Resultsmentioning

confidence: 77%

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The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

Clowez

Godaux

Cardol

et al. 2015

Journal of Biological Chemistry

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Background: Shift to anoxia promotes the over-reduction of photosystem I electron acceptors. Results: This over-reduction can be relieved by the activation of several pathways. Conclusion: The two mains pathways are the ATP-dependent CO 2 fixation pathway and the ATP-independent hydrogenase. Significance: We disentangle the role of the various NADPH-consuming pathways in setting the redox poise in the chloroplast of unicellular photosynthetic algae.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 77%

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

Clowez

Godaux

Cardol

et al. 2015

Journal of Biological Chemistry

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“…2, A and B, cluster A1). Genes in this cluster encode proteins that may be involved in metabolic processes related to oxygen sensing that are mediated through a heme-or ironbased sensor (Aravind and Koonin, 2001;Zhu and Bunn, 2001;Quinn et al, 2002). There were also genes that were induced by hypoxia but not hypobaria (Fig.…”

Section: Differential Expression Of Genes On the Arraymentioning

confidence: 99%

“…2B, cluster A1). The genes representing this group encode several putative proteins that may represent metabolic processes related to oxygen sensing that are mediated through a heme-or iron-based sensor (Quinn et al, 2002). Iron-based sensors have been implicated for direct oxygen or indirect redox sensing in various hypoxia-responsive pathways in eukaryotes (Bunn et al, 1998;Zhu and Bunn, 2001;Mecklenburgh et al, 2002), relying in part on subtilisin-catalyzed proteolytic inactivation of p450 that functions in the reduction of Fe(III) in the signal transduction cascade (Aravind and Koonin, 2001).…”

Section: Hypobaria Does Not Equal Hypoxiamentioning

confidence: 99%

Hypobaric Biology: Arabidopsis Gene Expression at Low Atmospheric Pressure

et al. 2004

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(M.P.P.) As a step in developing an understanding of plant adaptation to low atmospheric pressures, we have identified genes central to the initial response of Arabidopsis to hypobaria. Exposure of plants to an atmosphere of 10 kPa compared with the sea-level pressure of 101 kPa resulted in the significant differential expression of more than 200 genes between the two treatments. Less than one-half of the genes induced by hypobaria are similarly affected by hypoxia, suggesting that response to hypobaria is unique and is more complex than an adaptation to the reduced partial pressure of oxygen inherent to hypobaric environments. In addition, the suites of genes induced by hypobaria confirm that water movement is a paramount issue at low atmospheric pressures, because many of gene products intersect abscisic acid-related, drought-induced pathways. A motivational constituent of these experiments is the need to address the National Aeronautics and Space Administration's plans to include plants as integral components of advanced life support systems. The design of bioregenerative life support systems seeks to maximize productivity within structures engineered to minimize mass and resource consumption. Currently, there are severe limitations to producing Earth-orbital, lunar, or Martian plant growth facilities that contain Earth-normal atmospheric pressures within light, transparent structures. However, some engineering limitations can be offset by growing plants in reduced atmospheric pressures. Characterization of the hypobaric response can therefore provide data to guide systems engineering development for bioregenerative life support, as well as lead to fundamental insights into aspects of desiccation metabolism and the means by which plants monitor water relations.Interest in the exploration of environments beyond Earth's atmosphere has brought unique challenges to bear on the understanding of the biological systems that will inhabit those environments. Among these challenges are alterations in atmospheric pressure, which are known to have effects on plant physiology and development (Mansell et al., 1968; Gale, 1973; Andre and Richaux, 1986;McKay and Toon, 1991; Andre and Massimino, 1992;Wheeler, 2000; He et al., 2003). Concepts for greenhouses on Mars, on the moon, and in Earth orbit incorporate low atmospheric pressures to address engineering and systems limitations (Boston, 1981; Drysdale, 2001).Historically, low-pressure environments have been used throughout the U.S. human space exploration programs to reduce the masses of structural and consumable components of space vehicles. Such reductions have resulted in increased mission lengths and/or increased masses of launched payloads. For example, the Mercury, Gemini, and Apollo environments were designed to operate at 34 kPa with a pure oxygen environment to simplify support of humans in space (Baker, 1981;Martin and McCormick, 1992). Skylab was also operated at 34 kPa (with a 70% O 2 /30% N 2 gas mixture), and that pressure was further reduced during period...

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“…This alga possesses, however, two AcsF homologue isoforms (Crd1 and Cth1) (37,38). The crd1 gene was shown to be induced under low oxygen tension, and a CRD1 mutant is chlorotic under such conditions, which indicates that chlorophyll is supplied via Crd1.…”

Section: Discussionmentioning

confidence: 99%

Global Regulation of Photosynthesis and Respiration by FnrL

Ouchane

Picaud

Therizols

et al. 2007

Journal of Biological Chemistry

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Fnr is a regulator that controls the expression of a variety of genes in response to oxygen limitation in bacteria. To assess the role of Fnr in photosynthesis in Rubrivivax gelatinosus, a strain carrying a null mutation in fnrL was constructed. It was unable to grow anaerobically in the light, but, intriguingly, it was able to produce photosynthetic complexes under high oxygenation conditions. The mutant lacked all c-type cytochromes normally detectable in microaerobically-grown wild type cells and accumulated coproporphyrin III. These data suggested that the pleiotropic phenotype observed in FNR is primarily due to the control at the level of the HemN oxygen-independent coproporphyrinogen III dehydrogenase. hemN expression in trans partially suppressed the FNR phenotype, as it rescued heme and cytochrome syntheses. Nevertheless, these cells were photosynthetically deficient, and pigment analyses showed that they were blocked at the level of Mg 2؉ -protoporphyrin monomethyl ester. Expression of both hemN and bchE in the FNR mutant restored synthesis of Mg 2؉ -protochlorophyllide. We, therefore, conclude that FnrL controls respiration by regulating hemN expression and controls photosynthesis by regulating both hemN and bchE expression. A comprehensive picture of the control points of microaerobic respiration and photosynthesis by FnrL is provided, and the prominent role of this factor in activating alternative gene programs after reduction of oxygen tension in facultative aerobes is discussed.Anoxygenic photosynthetic bacteria can develop the required machinery for growth under aerobic respiration, anaerobic respiration, or photosynthesis under anaerobiosis and light. This faculty requires tight control to ensure production of the required complexes and rapid adaptation to changes in environmental conditions. Under anaerobiosis and light, purple bacteria perform anoxygenic photosynthesis on the basis of a bacteriochlorophyll-mediated process. It takes place within the membrane-bound photosynthetic apparatus composed of pigment-protein complexes (reaction center (RC) 2 and light harvesting (LH)) and involves membrane cytochromes. Synthesis of tetrapyrroles (heme and chlorophyll) is then a crucial process necessary for photosynthesis and respiratory growth of these organisms. Heme synthesis and its regulation have been studied in many organisms (for review, see Refs. 1-4). A key step in this pathway corresponds to the conversion of coproporphyrinogen III to protoporphyrinogen IX. In facultative aerobic prokaryotes, two structurally different enzymes catalyze this reaction depending on the oxygenation level. Under high oxygenation, the oxidation is catalyzed in Escherichia coli by the oxygen-dependent coproporphyrinogen III oxidase (HemF). Under anaerobiosis, an oxygen-independent coproporphyrinogen III dehydrogenase (HemN) is required (5, 6). Bacteriochlorophyll synthesis and its regulation have also been studied in many phototrophs (for review, see Ref. 7). A key step in the chlorophyll pathway in facultative aer...

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Oxygen Deficiency Responsive Gene Expression in Chlamydomonas reinhardtii through a Copper-Sensing Signal Transduction Pathway

Cited by 36 publications

References 18 publications

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

The Involvement of Hydrogen-producing and ATP-dependent NADPH-consuming Pathways in Setting the Redox Poise in the Chloroplast of Chlamydomonas reinhardtii in Anoxia

Hypobaric Biology: Arabidopsis Gene Expression at Low Atmospheric Pressure

Global Regulation of Photosynthesis and Respiration by FnrL

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