Oxygen Effects in Hairy Root Culture

Yu, Shaoxiong; Mahagamasekera, M.G.P.; Williams, Gary R.; Kanokwaree, Kanokwan; Doran, Pauline M.

doi:10.1201/9780367810610-13

Cited by 3 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complete absence of identified oxidative products in C. roseus could be due to one of several reasons. Studies with hairy roots have shown that their characteristic of growing in clumps could significantly reduce the concentration of oxygen available to them (Yu et al, 1997). Experiments have shown oxygen deprivation of the roots in shake flask cultures is a natural phenomenon, and this manifests as deficient growth of the roots (Yu et al, 1997).…”

Section: 42: Oxidationmentioning

confidence: 99%

“…Studies with hairy roots have shown that their characteristic of growing in clumps could significantly reduce the concentration of oxygen available to them (Yu et al, 1997). Experiments have shown oxygen deprivation of the roots in shake flask cultures is a natural phenomenon, and this manifests as deficient growth of the roots (Yu et al, 1997). Cytochrome P450 monooxygenases need dioxygen, both as a substrate and probably as an inducer as well (Schuler, 1996), and hence the observed low oxygen levels could result in their non-activation.…”

Section: 42: Oxidationmentioning

confidence: 99%

See 1 more Smart Citation

TNT phytotransformation in native and genetically modified species: significance of aromatic hydroxylamines in the metabolic pathway

Subramanian¹

View full text Add to dashboard Cite

The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleed-through, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.

show abstract

Section: 42: Oxidationmentioning

confidence: 99%

Section: 42: Oxidationmentioning

confidence: 99%

TNT phytotransformation in native and genetically modified species: significance of aromatic hydroxylamines in the metabolic pathway

Subramanian¹

View full text Add to dashboard Cite

show abstract

Hairy Root Culture in a Liquid-Dispersed Bioreactor: Characterization of Spatial Heterogeneity

Williams

Doran

2000

Biotechnol. Prog.

View full text Add to dashboard Cite

A liquid-dispersed reactor equipped with a vertical mesh cylinder for inoculum support was developed for culture of Atropa belladonna hairy roots. The working volume of the culture vessel was 4.4 L with an aspect ratio of 1.7. Medium was dispersed as a spray onto the top of the root bed, and the roots grew radially outward from the central mesh cylinder to the vessel wall. Significant benefits in terms of liquid drainage and reduced interstitial liquid holdup were obtained using a vertical rather than horizontal support structure for the biomass and by operating the reactor with cocurrent air and liquid flow. With root growth, a pattern of spatial heterogeneity developed in the vessel. Higher local biomass densities, lower volumes of interstitial liquid, lower sugar concentrations, and higher root atropine contents were found in the upper sections of the root bed compared with the lower sections, suggesting a greater level of metabolic activity toward the top of the reactor. Although gas-liquid oxygen transfer to the spray droplets was very rapid, there was evidence of significant oxygen limitations in the reactor. Substantial volumes of non-free-draining interstitial liquid accumulated in the root bed. Roots near the bottom of the vessel trapped up to 3-4 times their own weight in liquid, thus eliminating the advantages of improved contact with the gas phase offered by liquid-dispersed culture systems. Local nutrient and product concentrations in the non-free-draining liquid were significantly different from those in the bulk medium, indicating poor liquid mixing within the root bed. Oxygen enrichment of the gas phase improved neither growth nor atropine production, highlighting the greater importance of liquid-solid compared with gas-liquid oxygen transfer resistance. The absence of mechanical or pneumatic agitation and the tendency of the root bed to accumulate liquid and impede drainage were identified as the major limitations to reactor performance. Improved reactor operating strategies and selection or development of root lines offering minimal resistance to liquid flow and low liquid retention characteristics are possible solutions to these problems.

show abstract

Alkaloid Production by Hairy Root Cultures

Zhao

2014

View full text Add to dashboard Cite

Oxygen Effects in Hairy Root Culture

Cited by 3 publications

References 1 publication

TNT phytotransformation in native and genetically modified species: significance of aromatic hydroxylamines in the metabolic pathway

TNT phytotransformation in native and genetically modified species: significance of aromatic hydroxylamines in the metabolic pathway

Hairy Root Culture in a Liquid-Dispersed Bioreactor: Characterization of Spatial Heterogeneity

Alkaloid Production by Hairy Root Cultures

Contact Info

Product

Resources

About