Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer

Sánchez-Ajofrín, Irene; Iniesta‐Cuerda, María; Sánchez‐Calabuig, María Jesús; Peris-Frau, Patricia; Martín-Maestro, Alicia; Ortíz, José Antonio; Fernández-Santos, M.R.; Garde, José Julián; Gutiérrez‐Adán, Alfonso

doi:10.1016/j.anireprosci.2020.106279

Cited by 17 publications

(16 citation statements)

References 54 publications

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“…Further efforts to perform embryo transfer and production from this selected deer are oriented toward the acceleration of their genetic improvement rates. During the last two years, some papers revealed methodology concerning red deer in vitro production [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is important to determine the average number of ovarian follicles with dense and cohesive cumulus and uniformly granulated cytoplasm as an important criterion of oocyte quality and reproductive competence in hinds. Transcription factors, such as placenta-specific 8 (PLAC8), OCT4, and SOX2, which have been proposed for determining embryo quality parameters in IVF, are expressed in the bovine, ovine, and deer blastocysts obtained from the oocytes used for IVF [6,14,16]. The presence of programmed cell death might lead to oocyte degeneration [17], and early embryonic death [16].…”

Section: Introductionmentioning

confidence: 99%

“…The Cu-SOD and Zn-SOD (SOD1) are typically cytosolic, and the Mn-SOD (SOD2) is mitochondrial, while the extracellular SOD (SOD3) scavenges superoxide radicals in extracellular fluids and spaces [28]. According to Sanchez-Ajofrin et al oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in red deer [6].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

Korzekwa

Kotlarczyk

Szczepańska

et al. 2020

Animals

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The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovaries collected post mortem on the 4th and 13th days of the estrous cycle and during pregnancy (n = 18). After oocyte maturation, frozen-thawed epididymal semen was used for IVF. Blastocyst quality, apoptotic potential by determining the mRNA expression of BAX, BCL-2, OCT4, SOX2, and placenta-specific 8 gene (PLAC8), and antioxidative potential of blastocysts were evaluated by determining the mRNA expression of CuSOD, MnSOD, and GPX as well as the enzymatic activity of superoxide dismutase and reduced glutathione. The highest development rate of expanded blastocyst, mRNA expression of BCL-2, OCT4, SOX2, and PLAC8 and mRNA expression and enzymatic activity of the antioxidative factors increased (p < 0.05) in blastocysts developed from the oocytes collected on the 4th day, compared to those developed from the oocytes collected on the 13th day of the cycle and during pregnancy. Our study indicates that the 4th day of the estrous cycle is the most effective period for oocyte collection for IVF and embryo development in hinds, considering quality parameters and antioxidative potential of the blastocysts.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

Korzekwa

Kotlarczyk

Szczepańska

et al. 2020

Animals

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“…It has been reported that cells are able to increase the expression of these enzymes when the concentration of oxygen radicals increases as a mechanism to counteract oxidative stress 35 , 36 . Similarly, several studies reported that the expression of these genes was downregulated when the oxygen concentrations during embryo culture were reduced and, thus, the ROS concentrations were low 37 – 39 . On the other hand, when embryos were exposed to increased ROS levels, these genes were upregulated 40 , 41 .…”

Section: Discussionmentioning

confidence: 83%

N-(2-mercaptopropionyl)-glycine enhances in vitro pig embryo production and reduces oxidative stress

Cambra

Martínez

Rodriguez‐Martinez

et al. 2020

Sci Rep

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This study evaluated the effects of different concentrations (1, 10, 25, 50, and 100 µM) of the antioxidant N-(2-mercaptopropionyl)-glycine (NMPG), during the culture of in vitro-fertilized porcine oocytes. While the highest concentrations of NMPG (50 and 100 µM) were toxic to the developing embryos during the first two days of culture, 25 µM NMPG achieved cleavage rates that were similar to those achieved by the control but did not sustain blastocyst production by Day 7 of culture. Compared to the control culture medium, the culture medium supplemented with 10 µM NMPG increased (P < 0.05) the rates of blastocyst formation, decreased (P < 0.05) the intracellular levels of reactive oxygen substances, and downregulated (P < 0.05) the expression of the oxidative stress related gene GPX1. In conclusion, these results demonstrated that supplementation of porcine embryo culture medium with 10 µM NMPG can attenuate oxidative stress and increase the yield of in vitro production of blastocysts.

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“…This treatment increased expression levels of mRNA for Fdf9 and Sirt3 , and reduced those for Sod2 in oocytes, suggesting a beneficial effect on oocyte quality and oxidative stress. Alterations in Sod2 expression have been reported in oocytes of different mammals under different conditions [ 39 , 40 ]. At the oocyte level, DCI is the substrate of phosphatidylinositol-3-kinase (PI3K), an enzyme involved in oocyte activation and in the survival and activity of granulosa cells on which oocyte quality depends, and is crucial for oocyte and preimplantation development [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome

Pericuesta

Laguna‐Barraza

Ramos‐Ibeas

et al. 2020

IJMS

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Polycystic ovarian syndrome (PCOS) is the main cause of female infertility. It is a multifactorial disorder with varying clinical manifestations including metabolic/endocrine abnormalities, hyperandrogenism, and ovarian cysts, among other conditions. D-Chiro-inositol (DCI) is the main treatment available for PCOS in humans. To address some of the mechanisms of this complex disorder and its treatment, this study examines the effect of DCI on reproduction during the development of different PCOS-associated phenotypes in aged females and two mouse models of PCOS. Aged females (8 months old) were treated or not (control) with DCI for 2 months. PCOS models were generated by treatment with dihydrotestosterone (DHT) on Days 16, 17, and 18 of gestation, or by testosterone propionate (TP) treatment on the first day of life. At two months of age, PCOS mice were treated with DCI for 2 months and their reproductive parameters analyzed. No effects of DCI treatment were produced on body weight or ovary/body weight ratio. However, treatment reduced the number of follicles with an atretic cyst-like appearance and improved embryo development in the PCOS models, and also increased implantation rates in both aged and PCOS mice. DCI modified the expression of genes related to oocyte quality, oxidative stress, and luteal sufficiency in cumulus-oocyte complexes (COCs) obtained from the aged and PCOS models. Further, the phosphorylation of AKT, a main metabolic sensor activated by insulin in the liver, was enhanced only in the DHT group, which was the only PCOS model showing glucose intolerance and AKT dephosphorylation. The effect of DCI in the TP model seemed mediated by its influence on oxidative stress and follicle insufficiency. Our results indicate that DCI works in preclinical models of PCOS and offer insight into its mechanism of action when used to treat this infertility-associated syndrome.

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Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer

Cited by 17 publications

References 54 publications

Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor

N-(2-mercaptopropionyl)-glycine enhances in vitro pig embryo production and reduces oxidative stress

D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome

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