Oxygen tensions in multicell spheroids of two cell lines

Binding of the hypoxia tracer [3H]misonidazole in cerebral ischemia.

“…2 Electrode measurements have shown that Po 2 in spheroids of this size decreased from 100-130 mm Hg at the spheroid surface to 0-3 mm Hg at or near the center of V-79 spheroids and 5-25 mm Hg in EMT-6 spheroids. 16 Several mouse tumors in vivo behave similarly, with most radiolabel bound in cells directly adjacent to necrosis, which occurs 150-200 /xm from blood vessels and is presumed due to insufficient O 2 for cell growth 2 (J.S. Rasey, unpublished observations).…”

Necrotic core in EMT6/Ro tumour spheroids: Is it caused by an ATP deficit?

“…We remark that the values of the diffusivities (see Table 1) guarantee that the contribution of the extracellular liquid flow to metabolite transport is negligible. Experimental observations that evidenced the presence of concentration gradients in a layer surrounding the surface of tumour spheroids (Mueller-Klieser and Sutherland, 1982, Mueller-Klieser, 1984, Groebe and Mueller-Klieser, 1991, led us to prescribe the concentrations in the culture medium at a fixed distance L from the spheroid surface. These concentrations obey the following equations:…”

“…The growth characteristics and the metabolism of the EMT6/Ro spheroid (mouse mammary carcinoma cells) have been extensively investigated (Mueller-Klieser and Sutherland, 1982, Mueller-Klieser, 1984, Freyer and Sutherland, 1985, Freyer and Sutherland, 1986a, Casciari et al, 1988, Freyer, 1988, Walenta et al, 1990, Freyer et al, 1991, Casciari et al, 1992, Casciari et al, 1992a. In Freyer and Sutherland (1986a) in particular, the radius of the necrotic core for spheroids of different size and under different oxygen and glucose concentrations in the medium were reported.…”

Comparative pO2 measurements in cell spheroids cultured with different techniques

“…However, in different publications quite different central P02 values and shapes of pO2 gradients have been reported (Carlsson et al, 1979;Carlsson & Acker, 1985;Kaufman et al, 1981: Mueller-Klieser & Sutherland, 1982bSutherland et al, 1986). Different laboratories have used different cell types to form the spheroids and also applied different culture techniques.…”