2020
DOI: 10.1002/jctb.6420
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Oxygen transfer rate determines molecular weight and production of poly(γglutamic acid) as well as carbon utilization by Bacillus velezensis 83

Abstract: BACKGROUNDThe objective of the study was to evaluate the influence of the oxygen transfer rate (OTR) on poly(γ‐glutamic acid) (γ‐PGA) production and molecular weight and the carbon source utilization in cultures of Bacillus velezensis 83 developed in shake flasks.RESULTSThe cellular metabolism of B. velezensis 83 was affected by the OTR conditions. At maximum oxygen transfer rates (OTRmax) of 12, 17 and 19 mmol L−1 h−1, glucose was mainly used for cell growth and γ‐PGA production. In contrast, under oxygen‐lim… Show more

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Cited by 9 publications
(12 citation statements)
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“…This may be due to an increased γ‐PGA production under oxygen‐limited cultivation conditions 30 . Possibly, it also may depend on the higher molecular weight of the produced γ‐PGA induced by the changed cultivation conditions 1,30,44 …”
Section: Resultsmentioning
confidence: 99%
“…This may be due to an increased γ‐PGA production under oxygen‐limited cultivation conditions 30 . Possibly, it also may depend on the higher molecular weight of the produced γ‐PGA induced by the changed cultivation conditions 1,30,44 …”
Section: Resultsmentioning
confidence: 99%
“…Glucose in supernatants was determined using a biochemistry analyzer (YSI, Yellow Springs Instruments, Ohio, USA). PGA concentration and molecular weight were measured using gel permeation chromatography with a serial set of ultrahydrogel columns (UG 500 and Linear, Waters), using a highperformance liquid chromatography system (Waters 2414) equipped with a differential refractometer detector (Waters 410) as reported by Flores et al 30 Surfactin and bacillomycin in the culture broth were determined according to Balderas-Ruíz et al 35 Surface tension was measured in the culture broth previously centrifuged (10 000 rpm, 10 min), using a Wilhelmy plate tensiometer (ST-PLUS, Tantech Inc., USA) as previously described by Brito-Bazán et al, 13 at the temperature at which the fermentations were carried out (30 °C). The rheological properties of the broth were measured using a stress-controlled DHR-3 (TA Instruments) rheometer, using a concentric cylinder with double gap, at a shear rate of 100 s −1 and at 30 °C.…”
Section: Methodsmentioning
confidence: 99%
“…CP034203) was provided by Agro&Biotecnia S de RL de CV. The strain was kept at −18 °C in 80% (w/w) glycerol–water before use and recovered in a Petri dish with YPG solid medium containing (in g L −1 ): yeast extract, 10; peptone, 10; glucose, 10; agar, 15 30 …”
Section: Methodsmentioning
confidence: 99%
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