Oxygen Uptake Rate Controlling Strategy Balanced with Oxygen Supply for Improving Coenzyme Q10 Production by Rhodobacter sphaeroides

Wang, Zejian; Zhang, Xingzi; Wang, Ping; Sui, Zhiwei; Guo, Meijin; Zhang, Siliang; Zhuang, Yingping

doi:10.1007/s12257-019-0461-3

Cited by 5 publications

(2 citation statements)

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“…Recently, the α-proteobacterium Rhodobacter sphaeroides , a facultative photosynthetic bacterium [ 8 ] with promising potential, has been significantly advanced as a novel MCF for industrial applications [ 9 ]. Because of its high metabolic versatility ( e.g., photoautotrophic, photoheterotrophic and chemoheterotrophic), a variety of value-added products, such as coenzyme Q 10 (Q 10 ) [ [10] , [11] , [12] , [13] ], isoprenoids ( i.e. , lycopene and β-carotene) [ [14] , [15] , [16] , [17] ], 5-amino-ketone pentyl acid [ 18 ] and even bio-hydrogen [ 19 , 20 ] can be efficiently produced in this cell factory.…”

Section: Introductionmentioning

confidence: 99%

“…R. sphaeroides JDW-710 (JDW-710) is a high-yielding industrial strain used for Q 10 production [ 12 ], and it is also a nonpathogenic and generally regarded as safe (GRAS) microbial strain [ 9 ]. In this study, on the basis of transcriptomic analysis of JDW-710 and the use of a red fluorescent protein (RFP) reporter system, a native promoter-derived synthetic promoter library incorporating a range of different strengths was characterized.…”

Section: Introductionmentioning

confidence: 99%

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Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides

Shi

Zhang

Liang

et al. 2021

Synthetic and Systems Biotechnology

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The versatile photosynthetic α-proteobacterium Rhodobacter sphaeroides , has recently been extensively engineered as a novel microbial cell factory (MCF) to produce pharmaceuticals, nutraceuticals, commodity chemicals and even hydrogen. However, there are no well-characterized high-activity promoters to modulate gene transcription during the engineering of R. sphaeroides . In this study, several native promoters from R. sphaeroides JDW-710 (JDW-710), an industrial strain producing high levels of co-enzyme Q 10 (Q 10 ) were selected on the basis of transcriptomic analysis. These candidate promoters were then characterized by using gusA as a reporter gene. Two native promoters, P rsp _ 7571 and P rsp _ 6124 , showed 620% and 800% higher activity, respectively, than the tac promoter, which has previously been used for gene overexpression in R. sphaeroides. In addition, a P rsp _ 7571 -derived synthetic promoter library with strengths ranging from 54% to 3200% of that of the tac promoter, was created on the basis of visualization of red fluorescent protein (RFP) expression in R. sphaeroides . Finally, as a demonstration, the synthetic pathway of Q 10 was modulated by the selected promoter T334* in JDW-710; the Q 10 yield in shake-flasks increased 28% and the production reached 226 mg/L . These well-characterized promoters should be highly useful in current synthetic biology platforms for refactoring the biosynthetic pathway in R. sphaeroides -derived MCFs.

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