Oxytocin receptor signalling

Devost, Dominic; Wrzal, Paulina K.; Zingg, Hans H.

doi:10.1016/s0079-6123(08)00415-9

Cited by 53 publications

(44 citation statements)

References 24 publications

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“…Furthermore, OT angiogenic and antiapoptotic effects were postulated upon the observation of increased cardiac CD31 ϩ microvessels in ovariectomized rats (29). Our observations in MSC that OT treatment activates the ERK1/2 pathway is also supported by the finding that p38 MAPK and ERK1/2 phosphorylation enhance the proliferative activity of uterine cells (30).…”

Section: Discussionsupporting

confidence: 60%

Preconditioning of Stem Cells by Oxytocin to Improve Their Therapeutic Potential

et al. 2012

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Principal limitation of cell therapy is cell loss after transplantation because of the interplay between ischemia, inflammation, and apoptosis. We investigated the mechanism of preconditioning of mesenchymal stem cells (MSCs) with oxytocin (OT), which has been proposed as a novel strategy for enhancing therapeutic potential of these cells in ischemic heart. In this study, we demonstrate that rat MSCs express binding sites for OT receptor and OT receptor transcript and protein as detected by RT-PCR and immunofluorescence, respectively. In response to OT (10(-10) to 10(-6) M) treatment, MSCs respond with rapid calcium mobilization and up-regulation of the protective protein kinase B (PKB or Akt) and phospho-ERK1/2 proteins. In OT-stimulated cells, phospho-Akt accumulates intracellularly close to the mitochondrial marker cytochrome c oxidase subunit 4. Functional analyses reveal the involvement of Akt/ERK1/2 pathways in cell proliferation, migration, and protection against the cytotoxic and apoptotic effects of hypoxia and serum deprivation. In addition, OT preconditioning increases MSC glucose uptake. Genes with angiogenic, antiapoptotic, and cardiac antiremodeling properties, such as heat shock proteins (hsps) HSP27, HSP32, HSP70, vascular endothelial growth factor, thrombospondin, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, TIMP-3, and matrix metalloproteinase-2, were also up-regulated upon OT exposure. Moreover, coculture with OT-preconditioned MSC reduces apoptosis, as measured using terminal transferase dUTP nick end labeling assay in newborn rat cardiomyocytes exposed to hypoxia and reoxygenation. In conclusion, these results indicate that OT treatment evokes MSC protection through both intrinsic pathways and secretion of cytoprotective factors. Ex vivo cellular treatment with OT represents an attractive strategy aimed to maximize the biological and functional properties of effector cells.

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Section: Discussionsupporting

confidence: 60%

Preconditioning of Stem Cells by Oxytocin to Improve Their Therapeutic Potential

et al. 2012

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“…Indeed, several reports have indicated that OT could activate complex intracellular networks and different kinases using different intermediates. For instance, phosphorylation of extracellular signal-regulated kinase 2 (ERK 1/2) and Akt may contribute to the cytoprotective and proliferative effects of OT [32]. In a rabbit model of myocardial ischemia-reperfusion injury, Kobayashi et al revealed that OT could improve cardiac functions via triggering cell-survival signals, such as pAkt, pSTAT3, pERK (extracellular signal-regulated kinase), and Bcl-2 [33].…”

Section: Discussionmentioning

confidence: 99%

Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats

Erbaş

Akman

Yavaşoğlu

et al. 2014

BioMed Research International

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Cisplatin (CP), an antitumor agent, has been shown to cause ovarian injury and dysfunction in both animal and human studies. The present study was conducted to investigate the protective effect of oxytocin (OT) on CP-induced ovarian toxicity in rats. Twenty-one adult female rats were included in the study. Fourteen rats were administered intraperitoneally CP (2 mg/kg/day) twice a week for 5 weeks. Control group (n = 7) did not receive any treatment. Following treatment, CP-received rats were randomly divided into two groups and treated with either saline (1 mL/kg/day, n = 7) or OT (160 μg/kg/day, n = 7) for 5 weeks. Then, ovarian toxicity and effects of OT were evaluated by histomorphological and biochemical analysis. Our findings revealed a significant reduction in the number of follicles at each grade in saline-treated group. AMH level was significantly lower in saline group compared to control (P < 0.0005). OT treatment significantly attenuated CP toxicity in ovaries and increased AMH levels compared to saline group (P < 0.005). Also, administration of OT lessened lipid peroxidation and prevented glutathione depletion in CP-treated rats (P < 0.05). These results indicated that OT could lessen the CP-induced ovarian damage and improve follicular reserve by preventing oxidative damage.

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“…This type of G-protein leads to a rise in intracellular calcium (Ca+) and a muscle cell contraction, of particular importance to milk let-down and uterine contractions (see Figure 1). 7,8 However, when the OTR is on a neuron, the response may be the subsequent release or inhibition of other hormonal neurotransmitters and modulators, such as serotonin, endogenous opioids and corticotrophin-releasing factor. 3 These nervous system interactions are key to understanding how oxytocin released in the brain influences a variety of mental states and behavior.…”

Section: Oxytocin Receptor: How Oxytocin Affects Physiology In the Brmentioning

confidence: 99%

Beyond Labor: The Role of Natural and Synthetic Oxytocin in the Transition to Motherhood (2014/023)

2014

J Midwife Womens Health

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Endogenous oxytocin is a key component in the transition to motherhood affecting molecular pathways that buffer stress reactivity, support positive mood, and regulate healthy mothering behaviors (including lactation). Synthetic oxytocin is widely used throughout labor and postpartum care in modern obstetrics. Yet research on the implications beyond labor of maternal exposure to perinatal synthetic oxytocin is rare. In this article, we review oxytocin-related biological pathways and behaviors associated with the transition to motherhood, and evidence supporting the need for further research on potential effects of intrapartum oxytocin beyond labor. We include a primer on oxytocin at the molecular level.

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Oxytocin receptor signalling

Cited by 53 publications

References 24 publications

Preconditioning of Stem Cells by Oxytocin to Improve Their Therapeutic Potential

Preconditioning of Stem Cells by Oxytocin to Improve Their Therapeutic Potential

Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats

Beyond Labor: The Role of Natural and Synthetic Oxytocin in the Transition to Motherhood (2014/023)

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