P Bodies and the Control of mRNA Translation and Degradation

Parker, Roy; Sheth, Ujwal

doi:10.1016/j.molcel.2007.02.011

Cited by 1,174 publications

(1,271 citation statements)

References 95 publications

(126 reference statements)

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“…Consistently, P bodies contain components involved in 5' to 3' mRNA degradation, including the decapping complex DCAP1/ DCAP2, decapping coactivators (e.g. RCK/p54, LSM1-7 complex, EDC3, Hedls and eIF4E-T), the CCR4/NOT1 deadenylase complex and the exonuclease XRN1 (Parker and Sheth, 2007). Components required for NMD (e.g.…”

Section: Introductionmentioning

confidence: 96%

“…Cytoplasmic processing bodies, termed P bodies, are involved in mRNA degradation, nonsense-mediated RNA decay (NMD), siRNA-and micro-RNA (miRNA)-mediated gene silencing in mammalian cells (Sheth and Parker, 2003;Cougot et al, 2004;Jakymiw et al, 2005;Liu et al, 2005aLiu et al, , 2005bSen and Blau, 2005;Bruno and Wilkinson, 2006;Parker and Sheth, 2007). Consistently, P bodies contain components involved in 5' to 3' mRNA degradation, including the decapping complex DCAP1/ DCAP2, decapping coactivators (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…SMG5, SMG7, UPF1), siRNA-and miRNA-mediated gene silencing (e.g. AGO, GW182 and RAP55) are also localized in P bodies (Parker and Sheth, 2007). The formation of P bodies is also regulated by components involved in these RNA metabolism processes (Parker and Sheth, 2007).…”

Section: Introductionmentioning

confidence: 99%

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A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi

et al. 2011

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Cytoplasmic processing bodies, termed P bodies, are involved in diverse post-transcriptional processes including mRNA decay, nonsense-mediated RNA decay (NMD), RNAi, miRNA-mediated translational repression and storage of translationally silenced mRNAs. Regulation of the formation of P bodies in the context of multicellular organisms is poorly understood. Here we describe a systematic RNAi screen in C. elegans that identified 224 genes with diverse cellular functions whose inactivations result in a dramatic increase in the number of P bodies. 83 of these genes form a complex functional interaction network regulating NMD. We demonstrate that NMD interfaces with many cellular processes including translation, ubiquitin-mediated protein degradation, intracellular trafficking and cytoskeleton structure. We also uncover an extensive link between translation and RNAi, with different steps in protein synthesis appearing to have distinct effects on RNAi efficiency. Moreover, the intracellular vesicular trafficking network plays an important role in the regulation of RNAi. A subset of genes enhancing P body formation also regulate the formation of stress granules in C. elegans. Our study offers insights into the cellular mechanisms that regulate the formation of P bodies and also provides a framework for system-level understanding of NMD and RNAi in the context of the development of multicellular organisms.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi

et al. 2011

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“…gene promoter methylation, represents a fundamental mechanism of physiological cell homeostasis. A few years ago, the epigenetic micro RNA (miRNA) system was identified as a post-transcriptional inhibitory regulator of mRNA translation [1][2][3]. These small non-amino acidcoding RNA molecules originate from miRNA-genederived hairpin primary miRNA transcripts of ∼80 nucleotides which are reduced in length during several intermediate stages and finally result in ∼20 nucleotidelong single-strand and now biologically active mature miRNA.…”

Section: Introductionmentioning

confidence: 99%

“…Mature miRNA are capable of semi-complementary binding to the 3′-untranslated region of target mRNA which results in suppression of mRNA translation/protein generation. This imperfect miRNA/mRNA base-pairing explains why one miRNA may have several potential mRNA targets and vice versa one mRNA may be targeted by more than one miRNA [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Megakaryocytic expression of miRNA 10a, 17-5p, 20a and 126 in Philadelphia chromosome-negative myeloproliferative neoplasm

et al. 2008

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International audienceMicro RNA (miRNA) are small non-coding RNA molecules which have a post-transcriptional inhibitory regulation function, e.g. in megakaryopoiesis. A characteristic of Philadelphia chromosome-negative myeloproliferative neoplasm (Ph MPN) is the abundance of morphologically aberrant megakaryocytes. Based on previously published in vitro megakaryocytic differentiation assay data, we selected miRNA 10a, 17-5p, 20a and 126 and potential target proteins (HOXA1, RUNX1) for analysis of laser-microdissected bone marrow megakaryocytes from Ph MPN and controls ( = 66). Furthermore, we tested a potential influence of cytoreductive treatment on miRNA expression in bone marrow cells during the course of Ph MPN ( = 18). In summary, miRNA 17-5p, 20a and 126 are constitutively expressed in Ph MPN megakaryopoiesis while low or absent miRNA 10a appeared to correlate with strong megakaryocytic HOXA1 protein expression. No association to thrombocytosis, JAK2 mutations or cytoreductive treatment (bone marrow cells) were observed

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Gemin5 plays a role in unassembled‐U1 snRNA disposal in SMN‐deficient cells

et al. 2018

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Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A are enriched in cytoplasmic granules and colocalize to P bodies in SMN-deficient cells. Immunoprecipitation assays show increased associations of the U1 snRNP component U1A with P body components and Gemin5 in SMN-deficient cells. More importantly, Gemin5 knockdown eliminates the unassembled U1 snRNP granules and rescues U1 snRNA levels in SMN-deficient cells. Taken together, our study provides direct evidence that Gemin5 is involved in unassembled-U1 snRNA disposal under conditions of SMN deficiency.

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P Bodies and the Control of mRNA Translation and Degradation

Cited by 1,174 publications

References 95 publications

A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi

A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi

Megakaryocytic expression of miRNA 10a, 17-5p, 20a and 126 in Philadelphia chromosome-negative myeloproliferative neoplasm

Gemin5 plays a role in unassembled‐U1 snRNA disposal in SMN‐deficient cells

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