P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation

Abstract

Background: P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was suggested an antioxidant role. Methods: We evaluated here the effect of topical treatment with P1G10, in mice lesions induced by UVB. Results: After single exposure to 2.4 J cm−2 UVB, P1G10 reduced erythema, increased cellularity of hypodermis, enhanced MPO activity and IL1β, and inhibited COX2 levels. These results point to an anti-inflammatory effect by P1G10. This fraction displayed antioxidant activity by reversing the depletion of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reducing the catalase activity increased by UVB. These changes may be related to a reduction in MDA observed in groups treated with P1G10. P1G10 also inhibited MMP-9, caspase-3 and pkat while increasing p53 levels.

Fullerenol protects cornea from ultraviolet B exposure

“…superoxide dismutase (SOD) or catalase) or small molecular antioxidants (e.g. glutathione (GSH) or vitamin E) were explored for the treatment of ocular disease [ 3 , 12 ]. However, based on their antioxidant mechanisms, most of these antioxidants have a limited spectrum for free radical scavenging [ 13 , 14 ].…”

“…For instance, SOD is particularly effective in scavenging superoxide anions (O 2 • − ) [ 15 , 16 ] and vitamin E is only responsible for peroxyl radicals (LOO•) deprivation [ 17 , 18 ]. More importantly, long-term exposure to UVB is found to significantly decrease the activity of enzymatic antioxidants [ 12 , 19 ], which further hinders its application in protecting UVB-induced ocular damage. Therefore, a suitable UV protector with broad-spectrum, effective free radical scavenging ability and good chemical stability is desirable.…”