p38 MAPK regulates PKAα and CUB-serine protease in Amphibalanus amphitrite cyprids

Abstract

The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKAα, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKAα was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKAα might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.

Proteome of larval metamorphosis induced by epinephrine in the Fujian oyster Crassostrea angulata

“…Previous studies have found that the p38 MAPK pathway was activated during the settlement process of barnacles [ 64 , 65 ]. In this study, protein ID c100749_g1 was identified as MAP kinase-activated protein kinase 2-like (Supplementary Table 5 ).…”

“…The classical signal pathway closely related to cell proliferation and apoptosis, the mitogen-activated protein kinase (MAPK) pathway, has also been well studied. Those pathways are involved in the regulation of settlement and metamorphosis of marine invertebrates [ 48 , 64 , 65 , 67 ].…”

Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

“…It has been hypothesized that SIPC binds tightly to shell components (such as chitin or minerals), because a strong buffer (containing SDS and DTT) is required to extract SIPC from barnacle shells 16 . Moreover, inhibition of p38 MAPK pathway 17 and elevation of endogenous nitric oxide concentration 18 both decrease the expression level of SIPC in cyprids. It is difficult to attribute these results to the two known functions of SIPC (pheromone and adhesive).…”

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways

“…DLD and NQO1 mRNA changes in this study were analysed after TKT knockdown. The non-canonical regulatory function of TKT likely caused these downstream changes since DLD expression has been reported to respond to manipulation of MAPK signalling [ 43 ]. In contrast, the NRF2 pathway, which regulates NQO1 expression, can also be controlled by EGFR [ 44 ].…”