P53 Expression During Multistage Human Oral Carcinogenesis

Zhang, L; Rosin, M; Priddy, Robert; Xiao, Yuxiu

doi:10.3892/ijo.3.4.735

Cited by 6 publications

(3 citation statements)

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“…This suggests either a limited involvement of this suppressor gene in the genomic instability (field cancerization) of the morphologically normal mucosa or the inability of current techniques to detect rare abnormal cells. Our results and those of others (Boyle et al, 1993;Chang et al, 1993;Levine, 1992;Pavelic et al, 1992;Soussi et al, 1994;Watling et al, 1992;Zhang et al, 1993), however, suggest that p53 alterations are restricted to detectable committed neoplastic cells and represent true mutation rather than polymorphism (Soussi et aZ., 1994).…”

Section: Sequencingcontrasting

confidence: 50%

“…Some investigators contend that p53 is an early event in the development of these lesions; others consider it a late step in their progression (Anwar et al, 1993;Brachman et al, 1992;Burns et al, 1993;Chang et al, 1992;Chung et al, 1993;Field et al, 1991;Gasparini et al, 1993;Landesman et al, 1994;Langey et al, 1993;Pavelic et al, 1992;Reiss et al, 1992;Warnakulasuriya and Johnson, 1992; Watling et al, 1992;Zhang et al, 1993). Most of these studies have been conducted on tumor cell lines (Burns et al, 1993;Chang et al, 1992;Yin et al, 1993) or advanced carcinoma samples (Chung et al, 1993;Field et al, 1991;Langey et al, 1993;Pavelic et al, 1992), using immunohistochemical, molecular or both techniques.…”

Section: Sequencingmentioning

confidence: 99%

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Sequential p53 mutation analysis of pre‐invasive and invasive head and neck squamous carcinoma

El‐Naggar¹,

Lai²,

Luna³

et al. 1995

Intl Journal of Cancer

119

101

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Single-stranded conformational polymorphism (SSCP) and direct sequencing were performed on uninvolved mucosa, severe dysplasia and invasive carcinoma samples from 20 patients with head and neck squamous carcinoma. Seven (35%) of the non-invasive lesions and 15 (75%) of the invasive carcinomas manifested p53 mutations. Although the majority of mutations were mis-sense, resulting in single amino acid substitution, a silent mutation encoding for the same amino acid and 2 non-sense mutations encoding a stop codon were also observed. Mutations in invasive carcinoma were mostly in exon 8 and involved codons 296, 288 and 298; non-invasive lesions showed more mutations at exons 5 to 7. Five lesions showed simultaneous mutations in 2 different exons; in 3 both non-invasive and invasive carcinomas showed primary mutation at exons 5 to 7, and invasive carcinoma showed a secondary mutation at exon 8. Different codon mutations in the same exon between dysplastic and the corresponding carcinoma samples were found in 2 cases. p53 alterations were not observed in any of the normal mucosa samples. No apparent association between p53 mutations and conventional clinicopathologic parameters, including DNA content, was found in this cohort. Our study indicates that (i) p53 alteration is an early event in the genesis of a subset of head and neck squamous carcinomas, (ii) normal mucosa within the resected specimens lacked p53 mutation, (iii) sequential mutations of different exons of the p53 gene suggests accumulation of genetic alterations during the neoplastic transformation of these lesions and (iv) the difference in codon mutation of the same exon between dysplastic and corresponding carcinoma suggests an independent clonal development.

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Section: Sequencingcontrasting

confidence: 50%

Section: Sequencingmentioning

confidence: 99%

Sequential p53 mutation analysis of pre‐invasive and invasive head and neck squamous carcinoma

El‐Naggar¹,

Lai²,

Luna³

et al. 1995

Intl Journal of Cancer

119

101

View full text Add to dashboard Cite

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“…in gastric carcinomas (Joypaul et al, 1993), prostatic carcinomas (Navone et al, 1993) or melanomas (Lassam et al, 1993), whereas in others, e.g. oral (Zhang et al, 1993), gall bladder (Kamel et al, 1993) and oesophageal (Wang et al, 1993) malignancies, abnormal p53 expression is a-n early event. In attempts to define the type and temporal sequence of somatic genetic changes that precede the onset of invasive lung cancer, recent studies have reported mutations and allelic deletions in the p53 gene in preinvasive bronchial lesions (Sozzi et al, 1992;Sundaresen et al, 1992).…”

mentioning

confidence: 99%

p53 expression in normal and dysplastic bronchial epithelium and in lung carcinomas

Walker

Robertson²,

Myskow³

et al. 1994

Br J Cancer

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S_mmary Bronchial epithelial dysplasia is thought to be a premalignant stage in the evolution of lung cancers. Using the CM-1 polyclonal antibody, we have examined the expression of the p53 protein in a larger series of bronchial dysplasias (n = 60) than hitherto investigated. The p53 protein was detected in 14% of mild, 25% of moderate and 59% of severe dysplasias; increased p53 expression correlated with the severity of dysplasia. p53-positive dysplasias had greater PCNA indices than p53-negative dysplasias. p53 expression in dysplastic tissues was compared with that in two groups of histologically normal epithelium: 14 bronchial biopsies from non-cancer patients of which all but one were negative and 32 bronchial margins from resecied carcinomas, of which 17 showed infrequent solitary cells with p53-positive nuclei in predominantly basal locations scattered throughout the epithelium. These results for resection margins were confirmed by use of a second antibody, DO-1. Sixty-nine per cent of the corresponding carcinomas were p53 positive, but in 15 cases the p53 reactivity differed from resection margins. No correlation between p53 expression and any of the clinicopathological characteristics of these tumours was found. This study supports the observation that abnormal p53 expression may be an early but not obligatory event in malignant transformation in lung.

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High prevalence of expression of p53 oncoprotein in oral carcinomas from India associated with betel and tobacco chewing

Kuttan

Rosin

Ambika

et al. 1995

European Journal of Cancer Part B: Oral Oncology

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P53 Expression During Multistage Human Oral Carcinogenesis

Cited by 6 publications

References 0 publications

Sequential p53 mutation analysis of pre‐invasive and invasive head and neck squamous carcinoma

Sequential p53 mutation analysis of pre‐invasive and invasive head and neck squamous carcinoma

p53 expression in normal and dysplastic bronchial epithelium and in lung carcinomas

High prevalence of expression of p53 oncoprotein in oral carcinomas from India associated with betel and tobacco chewing

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