P53 Expression in Response to Equigan Induced Testicular Injury and Oxidative Stress in Male Rat and the Possible Prophylactic Effect of Star Anise Extracts

Abstract

Objectives:Equigan is an anabolic androgenic steroid that developed for veterinary use to improve the food producing animal growth rate through promoting protein synthesis and muscle growth. The current study aimed to investigate the possible prophylactic effect of star anise extracts (SAE) response of to Equigan induced testicular injury, oxidative stress, P53 expression in male rats. Materials and Methods: Forty adult male rats were equally divided into four groups. 1 st Control El-Masry et al.; ARRB, 14(1): 1-8, 2017; Article no.ARRB.34318 2 group, while 2 nd group were rats receive orally SAE for 12 weeks. 3 rd group include rats that injected intramuscularly with Equigan for 12 weeks while 4 th group were co-treated group where rats injected with Equigan and SAE for 12 weeks. Results: Testis sections in Equigan treated rat induced abnormal arrangement of spermatogenesis cycles; disturbance and decrease in the spermatogenic cells, many of a syncytial cells were detected with marked decrease in sperms numbers and moderate depleted and degenerated Leydig cells. Testicular immunohistochemical observation after Equigan intramuscular injections showed a significant increase of the apoptotic protein p53. Co-administration of SAE with Equigan improved the testicular injury and P53 alternations. Conclusions: SAE could scavenge free radicals and produce beneficial effects against Equigan damage in testis and P53 alternations. Original Research Article

Star anise extracts modulation of reproductive parameters, fertility potential and DNA fragmentation induced by growth promoter Equigan in rat testes

“…These results agree with Pitts and Davis (2007) who reported that an evidence-based analysis of anabolic steroids also enhances performance in horses. Also, the current results agree with Barakat et al (2015) and El-Masry et al (2017).…”

“…Anabolic-androgenic steroids are defined as synthetic derivatives of the endogenous sex hormone testosterone that plays a key role in the development of male reproductive tissue (such as the testis and prostate) as well as in puberty, sexual behavior, spermatogenesis and plays promoting secondary sexual characteristics such as increased muscle, bone mass and hair growth (Mitchell et al, 2009;Balthazart, Ball, 2010;Mohammed et al, 2016). Anabolic steroids are forbidden for meat production and human use in KAS, and most countries worldwide due to their undesirable effects included blood and cardiovascular disorders (Santos et al, 2006;Tousson et al, 2016;Waljee et al, 2016), liver dysfunction (Van Amsterdam et al, 2010;Barakat et al, 2015), kidney disease (Barakat et al, 2015), tendon damage (Battista, Combs, Warne, 2003), testicular problems (Tousson et al, 2012;El-Masry et al, 2017), psychiatric and behavioural disorders in both sexes (Salas-Ramirez, Montalto, Sisk, 2010), as well as other problems on the human body (Saleh, Waded, 2014).…”

Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression

“…An efficient and competitive ACE-I, captopril is capable of reducing Ang II synthesis by acting as an antagonist to enzyme-converting angiotensin in plasma, tissue, and atherosclerotic plaque, as well as diminishing water-sodium retention, disrupting bradykinin breakdown, and regulating blood pressure by suppressing the production of aldosterone and thus inducing the dilation of the coronary and peripheral arteries [60,61]. When there is a lack of equilibrium between the ROS and antioxidant levels, the "redox" status of cells becomes imbalanced, which gives rise to oxidative stress [62,63]. In the external environment, ROS occur in pollutants, tobacco smoke, and radiation.…”

Amitriptyline Induced Alterations in Liver and Kidney Functions and Structures in Male Rats

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