p53 Staining Correlates With Tumor Type and Location in Sebaceous Neoplasms

Shalin, Sara C.; Sakharpe, Aniket; Lyle, Stephen; Lev, Dina; Calonje, Eduardo; Lazar, Alexander J.

doi:10.1097/dad.0b013e3181ed39f9

Cited by 47 publications

(39 citation statements)

References 40 publications

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“…The present study is in general agreement with work by Jakobiec, Kiyosaki, and Shalin [7,8,37]. Strong nuclear p53 staining was observed in SC in contrast to the sebaceous hyperplasia, sebaceous adenomas, and epithelioma.…”

Section: Discussionsupporting

confidence: 82%

“…While similar frequencies of p53 mutations have been found in both low-grade and high-stage SC, no significant correlation has been established between the severity of p53 mutation and tumor size, recurrence, metastasis, pagetoid spread, or location [36]. Recent work by Shalin et al has shown significant differences in staining between malignant SC and benign lesions [37]. Similarly demonstrating the genetic to tumor association, a report of a patient with Li-Fraumeni syndromeharboring a germ-line TP53 mutation-was recently reported to have SC [38].…”

Section: Genetic Featuresmentioning

confidence: 94%

“…The nuclei of tumor cells are typically round with granular or smudgy chromatin with small punctate nucleoli. These cells tended to have increased p53 staining patterns, with p53 staining being able to identify residual intraepithelial tumor cells [8,37].…”

Section: Genetic Featuresmentioning

confidence: 99%

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Clinical and Immunohistochemical Features of Sebaceous Carcinoma: Focusing on the p53 Tumor Suppressor

Singh¹,

Weinstock²,

Phelps³

et al. 2017

J Clin Exp Ophthalmol

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Purpose: Considerable focus has recently revealed the importance of p53 in sebaceous carcinoma (SC). This study investigates the utility of p53 as an immunohistochemistry (IHC) marker for differentiating benign and malignant sebaceous tissues and reports the clinical characteristics of patients diagnosed with SC at Mount Sinai Hospital (MSH). Methods:A retrospective chart analysis of 102 patients with SC was performed in addition to a histopathological examination of benign and malignant sebaceous tissues. Immunohistochemistry was performed in 30 cases of SC, 4 cases of sebaceous hyperplasia, 4 cases of sebaceous epitheliomas, and 3 cases of sebaceous adenoma. A separate outpatient cohort was used to compare the clinical and histologic features of eyelid lesions. This included 31 available cases with histologic descriptions of SC. The clinical features of 20 SC cases, 25 BCC cases, and 21 chalazia cases were available and compared from this outpatient cohort. Data collected included age, gender, lesion anatomical location, histopathologic descriptions, and percentage of p53 positive sebocytes. Results:Of the 102 SC cases at MSH, the mean age was 69 years, males composed 58%, and 59% were periorbital or orbital. Positive p53 staining without atypia was reproducibly observed at cell membranes in all cases of sebaceous hyperplasia. Using a standardized p53 grading system used at MSH, the average p53 staining grade for SC and epithelioma was calculated to be 2.3 ± 0.7 and 1 ± 0.7, respectively (p=0.002). An outpatient cohort revealed that 65% of SC was found on the lower eyelid and 84% of BCC was on the upper eyelid. The most common histologic features seen in SC were nuclear atypia (61%) and cytoplasmic vacuoles (55%). Conclusions:This study provides the first comprehensive staining profile of p53 in benign and malignant sebaceous tissues. We also report for the first time that membrane p53 staining was helpful and appeared to be highly specific for identifying sebaceous hyperplasia. p53 staining was also helpful for identifying SC and intraepithelial spreading tumor cells. The clinical and histologic characteristics of specific eyelid lesions in an outpatient setting are also shared. Lastly, this work incorporates the latest research to synthesize IHC recommendations for improving SC detection.

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Section: Discussionsupporting

confidence: 82%

Section: Genetic Featuresmentioning

confidence: 94%

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Clinical and Immunohistochemical Features of Sebaceous Carcinoma: Focusing on the p53 Tumor Suppressor

Singh¹,

Weinstock²,

Phelps³

et al. 2017

J Clin Exp Ophthalmol

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“…Our findings are largely concordant with prior publications, although earlier studies were smaller and did not specifically examine or report on intraepithelial tumor cells. With respect to p53, immunoreactivity has been reported in 8/15 SC cases (53%) [4], 17/17 cases (100%) [5], 22/42 cases (52%) [6], 4/10 cases (40%) [7] and 4/8 cases (50%) [8]. The combined incidence of p53 expression in these prior studies (55/92; 60%) is relatively similar to the 63% with moderate or strong immunoreactivity which we identified in the main tumor masses.…”

Section: Discussionmentioning

confidence: 99%

Expression of p16 and p53 in Intraepithelial Periocular Sebaceous Carcinoma

Bell

Singh

et al. 2015

Ocul Oncol Pathol

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Purpose: Identifying intraepithelial sebaceous carcinoma cells in small periocular biopsies can be difficult, particularly in the conjunctiva. The goal of this study was to evaluate p53 and p16 immunohistochemistry as potential markers of intraepithelial sebaceous carcinoma. Procedures: A total of 25 tumors, including 4 recurrent lesions, were stained for p16 and p53, with intensity scored as negative, weak, moderate or strong. Results: Expression of p16 was detected in intraepithelial sebaceous carcinoma cells in 24 of the 25 cases (96%), with only 1 case showing weak immunoreactivity. Intraepithelial p53 immunoreactivity was present in 17 of 25 tumors (68%), but was weak in 3 cases. Expression levels remained relatively stable in primary and recurrent tumors, but varied in a few cases between intraepithelial and subepithelial sites. Conclusions: Intraepithelial sebaceous carcinomas stained for p53 and p16 demonstrated moderate to strong immunoreactivity in 100% of cases for at least one of these proteins, suggesting that together they are useful markers for determining the extent of tumor spread. Of the two, p16 was immunoreactive in more cases than p53.

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“…30,31 Nuclear p53 staining was found to be significantly associated with the diagnosis of sebaceous carcinoma compared with benign sebaceous lesions. 32 Trichofolliculoma.-To date, vulvar trichofolliculoma in the literature is limited to a single series citing 3 cases. 33 All 3 were associated with vulvar intraepithelial neoplasia.…”

Section: Folliculosebaceousmentioning

confidence: 99%

Vulvar Adnexal Lesions: A 32-Year, Single-Institution Review From Massachusetts General Hospital

Baker

Selim

Hoang

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Because the skin and modified mucosal surfaces of the vulvar region contain dense apocrine glands and anogenital mammary-like glands, in addition to eccrine glands and folliculosebaceous units, benign as well as malignant lesions derived from these adnexal structures are, not surprisingly, found in the vulva. However, their incidence occurring in the vulva has not been reported, to our knowledge.Objective.-To determine the incidence of various vulvar adnexal lesions.Design.-We performed a retrospective review of the cases at our institution.Results.-A total of 189 vulvar adnexal lesions were identified. Most of these lesions were benign (133 of 189; 70%), with hidradenoma papilliferum being the most common, followed by syringoma and various types of cysts. Rare cases of tubular adenoma, poroma, spiradenoma, hidradenoma, cylindroma, sebaceoma, and trichoepithelioma were identified. Malignant adnexal neoplasms comprised the remaining 30% (56 of 189) of the cases. Extramammary Paget disease was the most common (49 of 56), and 29% (14 of 49) demonstrated an invasive component. Rare cases of basal cell carcinoma, sebaceous carcinoma, apocrine carcinoma, adenoid cystic carcinoma, and spiradenocarcinoma were identified.Conclusions.-In this retrospective review, we identified several benign entities that have not been previously reported on the vulva, namely pilomatricoma, poroma, spiradenoma, and sebaceoma. Hidradenoma papilliferum and extramammary Paget disease were the most common benign and malignant adnexal neoplasms, respectively. The spectrum of various vulvar adnexal lesions appears to reflect the frequency of the underlying glandular elements.

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p53 Staining Correlates With Tumor Type and Location in Sebaceous Neoplasms

Cited by 47 publications

References 40 publications

Clinical and Immunohistochemical Features of Sebaceous Carcinoma: Focusing on the p53 Tumor Suppressor

Clinical and Immunohistochemical Features of Sebaceous Carcinoma: Focusing on the p53 Tumor Suppressor

Expression of p16 and p53 in Intraepithelial Periocular Sebaceous Carcinoma

Vulvar Adnexal Lesions: A 32-Year, Single-Institution Review From Massachusetts General Hospital

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