p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase

Wu, Huiyun; Lan, Zhengdao; Li, Wenqing; Wu, Shechao; Weinstein, Jasminder; Sakamoto, Kathleen M.; Dai, Wei

doi:10.1038/sj.onc.1203803

Cited by 56 publications

(49 citation statements)

References 23 publications

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“…Binding between BubR1/Mad3 and Bub3 is constitutive, and is required for the localization of BubR1 to kinetochores during mitosis (Taylor et al, 1998). BubR1 can directly bind to Cdc20 in vitro and in vivo (Wu et al, 2000;Tang et al, 2001). Recombinant BubR1 protein purified from insect cells inhibits the activity of APC/C in ubiquitination assays more effectively than does recombinant Mad2 (Tang et al, 2001).…”

Section: Function Of Bubr1/mad3mentioning

confidence: 99%

The spindle checkpoint, aneuploidy, and cancer

2004

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Section: Function Of Bubr1/mad3mentioning

confidence: 99%

The spindle checkpoint, aneuploidy, and cancer

2004

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“…The activation of the SAC involves the formation of inhibitory complexes between Mad2 and/or Mad3/BubR1 and Cdc20, preventing Cdc20 from activating the APC/C (Hwang et al, 1998;Kim et al, 1998;Wu et al, 2000;Malmanche et al, 2006). The mitotic checkpoint complex (MCC), which contains either (1) BubR1 (Mad3 in yeast), Bub3, Mad2 and Cdc20-or (2) the BubR1-Bub3-Cdc20 and Mad2-Cdc20 subcomplexes (Fang et al, 1998;Hardwick et al, 2000;Fraschini et al, 2001;Millband and Hardwick, 2002;Tang et al, 2001;Sudakin et al, 2001;Fang, 2002).…”

Section: MCCmentioning

confidence: 99%

The RZZ Complex and the Spindle Assembly Checkpoint

Wang

et al. 2009

Cell Struct. Funct.

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ABSTRACT. The conserved protein Rod is found in various organisms. It is localized on the kinetochores or spindle microtubules during cell division. Rod is required for proper chromosome segregation during both mitosis and meiosis. The effects of rod mutations are similar for both equational and reductional divisions, giving rise to anaphases with lagging chromosomes and/or unequal numbers of chromosomes at the two poles. Recent studies have shown that Rod is a significant component of the mitotic checkpoint. It can form the RZZ complex with Zw10 and Zwilch, which plays an important role in maintaining a functional spindle assembly checkpoint.

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“…Cdc20 is an activating cofactor of APC/C during mitosis (8); an active mitotic checkpoint inhibits APC/C through APC/C-dependent polyubiquitylation of Cdc20 and subsequent degradation by the proteasome (12). BubR1 binds to and inhibits both Cdc20 (13) and APC/C itself (14), acting as a pseudosubstrate inhibitor that, depending on acetylation status, can be actively degraded by APC/C Cdc20 (15). The role of Bub3 in the MCC is unclear, although in fission yeast it is involved in MCC localization (16).…”

Section: Introductionmentioning

confidence: 99%

Caspase-3–Dependent Mitotic Checkpoint Inactivation by the Small-Molecule Inducers of Mitotic Slippage SU6656 and Geraldol

Riffell

Jänicke²,

Roberge³

2011

Molecular Cancer Therapeutics

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Microtubule-targeting cancer drugs such as paclitaxel block cell-cycle progression at mitosis by prolonged activation of the mitotic checkpoint. Cells can spontaneously escape mitotic arrest and enter interphase without chromosome segregation by a process termed mitotic slippage that involves the degradation of cyclin B1 without mitotic checkpoint inactivation. Inducing mitotic slippage with chemicals causes cells to die after multiple rounds of DNA replication without cell division, which may enhance the antitumor activity of microtubule-targeting drugs. Here, we explore pathways leading to mitotic slippage by using SU6656 and geraldol, two recently identified chemical inducers of mitotic slippage. Mitotic slippage induced by SU6656 or geraldol was blocked by the proteasome inhibitor MG-132 and involved proteasome-dependent degradation of cyclin B1 and the mitotic checkpoint proteins budding uninhibited by benzimidazole related 1 (BubR1) and cell division cycle 20 (Cdc20) in T98G cells. Mitotic slippage and the degradation of BubR1 and Cdc20 were also inhibited by the caspase-3 and -7 inhibitor DEVD-CHO.

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p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase

Cited by 56 publications

References 23 publications

The spindle checkpoint, aneuploidy, and cancer

The spindle checkpoint, aneuploidy, and cancer

The RZZ Complex and the Spindle Assembly Checkpoint

Caspase-3–Dependent Mitotic Checkpoint Inactivation by the Small-Molecule Inducers of Mitotic Slippage SU6656 and Geraldol

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