p722 ferrocifen loaded lipid nanocapsules improve survival of murine xenografted-melanoma via a potentiation of apoptosis and an activation of CD8+ T lymphocytes

Ferrocifen Loaded Lipid Nanocapsules: A Promising Anticancer Medication against Multidrug Resistant Tumors

“…This was confirmed on SK-Mel28 melanoma cells by Resnier et al, where P5 and DP1 had the same toxicity profiles as free compounds or were encapsulated into LNCs, with no variation of the IC 50 [ 59 ]. The same conclusion can be drawn for another melanoma cell line, B16F10, for free P722- or P722-loaded LNCs [ 96 ]. Moreover, several in vitro assessments showed that ferrocifens could induce both senescence and apoptosis depending on the concentration used.…”

“…This was confirmed by the counting of the apoptotic cells: 15% of apoptotic cells at 0.5 µM of DP1 compared to 5% at 0.1 µM [ 55 ]. Using a different method, Topin-Ruiz et al demonstrated that P722-loaded LNCs potentiated apoptosis via the intrinsic pathway: increase in procaspase 9, cleaved caspase 9 and procaspase 3 in B16F10 melanoma cells after treatment, opening up a new therapeutic strategy with encapsulated P722 [ 96 ].…”

“…Indeed, as previously described, P722-loaded LNCs were used on an orthotopic murine model of human B16F10 melanoma. In addition to an induction of apoptosis via the intrinsic pathway, the results revealed a significant increase in activated CD 8 + T lymphocytes after treatment of stealth P722-LNCs, leading to immunogenic cell death [ 96 ].…”

pH-Responsive Lipid Nanocapsules: A Promising Strategy for Improved Resistant Melanoma Cell Internalization

“…Our data suggest that, due to our formulation strategy, pH-sensitive nanovectors would be able to reverse MDR phenotype to improve cancer treatment. Furthermore, as previously studied by our group [57,[63][64][65], different post-insertion strategies applied to LNC offer prospects for obtaining conclusive results about the effective ability of these nanoparticles to be loaded with the substances of interest, such as DNA, RNA or other drugs as ferrocifens.…”

“…Some studies reported P(Vim) as a polycationic polymer able to efficiently deliver genetic material thanks to this buffering property [ 54 , 55 ]. Moreover, siRNA or ferrocifen loaded LNC developed in previous works have already shown a relevant therapeutic efficacy on melanoma tumor progression [ 56 , 57 ]: surface modification of these nanocarriers with C18H37-P(NVP-co-Vim) copolymers could, therefore, allow them to escape the endosome and would be suitable for this delivery application.…”