Paclitaxel-clusters coated with hyaluronan as selective tumor-targeted nanovectors

Synthetic Lipid Nanoparticles Targeting Steroid Organs

“…Lipidots were loaded with 3 tracers presenting similar physicochemical properties, that is, a slightly polar cyanine or cholesterol head and long C 16 or C 18 hydrophobic chains. Lipidots loaded with cholesterol were less stable than those loaded with cholesteryl stearate, indicating that the tracers used in this study were preferentially located in the particle core or profoundly anchored in the particle shell by their C 16 /C 18 tails.…”

“…Easily quantifiable analytic methods such as inductively coupled plasma mass spectroscopy are not applicable to organic nanoparticles. Hence, we turned to radiolabeling techniques based on the introduction of 14 C, 3 H, or 125 I radionuclides in polymer backbones (10)(11)(12), liposomes (13)(14)(15), or lipid nanoparticles (16)(17)(18). We triply labeled lipidots with 2 radiotracers and a fluorescent dye with close physicochemical properties favoring their colocalization in the particle's core, that is, a slightly polar head (cyanine or cholesterol moiety) and long hydrophobic chain or chains.…”

Paclitaxel-loaded hyaluronan solid nanoemulsions for enhanced treatment efficacy in ovarian cancer

“…Abraxane is a nonactive targeted delivery system that accumulates in tumors via an enhanced permeability and retention (EPR) effect. 8,12 Drugs lacking active cancer-targeting capacities often cause serious side effects and have low antitumor efficacy. Therefore, not only passive but also active tumor-targeting delivery systems for PTX-based chemotherapeutics are urgently needed.…”

“…Subsequently, 1 mL of each of the reconstituted PTX-SNs and PTX-HSNs (3 mg/mL) was added to the dialysis tubing attached to a support, and the release of PTX was determined according to Apparatus 2 of the Unites States Pharmacopeia (USP) dissolution method (paddle method). The release test was performed with 900 mL of the release medium at 100 rpm and 37°C; 1 mL of the released sample was collected at each time point (1,3,6,12,24,36,48,72,96,120, and 144 h). The content of PTX in each sample was measured using the HPLC method described.…”

Development of hyaluronic acid derivatives for applications in biomedical engineering

“…HA is widely used for active drug targeting to the tumour cells thanks to the expression of CD44 by the majority of solid tumours. For example, the chemotherapeutic agent Paclitaxel has been conjugated to the HA and afterwards released into the tumoral cells in multiple ways: via an ester linkage easily hydrolysed by enzymes present in the body [109]; via amino acid linkers using carbodiimide chemistry [110]; via esterification after modification of paclitaxel with 4-bromobutanoic acid [111]; encapsulation in nanoparticles afterwards covalently grafted to HA [112].…”