Paclitaxel loaded poly (DL lactic acid co castor oil) 60:40 with poloxamer‐F68 rod shape cylindrical nanoparticle preparation and in vitro cytotoxicity studies

Abstract

This research presents a thin‐film hydration‐solvent evaporation method to formulate the paclitaxel loaded poly (DL lactic acid co castor oil) 4:6 with poloxamer‐F68 cylindrical shape nanoparticles. The particles were less than 250 nanometers (nm) in size, with an average width of 60 nm and an average length of 100 nm. The percent yield, encapsulation efficiency (EE), and percent drug loading (DL) were detected. This approach produces drug loading values between 5% and 20% w/w. X‐ray powder diffraction (XRD) identified the physicochemical properties of nanoparticles differential scanning calorimetry (DSC) and Fourier‐transform infrared spectroscopy (FTIR). The investigation shows that the drug is molecularly dispersed in polymers or given in an amorphous or semicrystalline state. Horizontal water bath shaker technology considered the in vitro release of PTX loaded nanoparticles under sink conditions. Poly (DL lactic acid co castor oil) 4:6 nanoparticles exhibited a sustained release analysis. At the end of 30 hours, the percent cumulative drug release from the formulations was between 74.52% and 92.87% for F1 and F4. In vitro cytotoxicity assays indicate that PTX having p (DLLA:CO60:40) nanoparticles have a higher cytotoxic effect on MCF‐7/ADR.

Doxorubicin Hydrochloride Loaded Polyanhydride Nanoformulations and Cytotoxicity

“…The nanoformulations of DOXHClcontaining P-(DLLA-CO:60:40) polymers were developed by a modified solvent evaporation thin-film hydration technique as previously reported. [23][24][25] Four different drug concentrations with loading nanoformulations of DOXHCl, Pluronic® F-127 and polymer were dissolved in 15 ml of dichloromethane, which was evaporated in a vacuum rotary flash evaporator to form a thin film. The resulting velvety film was hydrated with 30 ml of 10 nM HEPES buffer (pH 7.4, 37°C, 4 hours).…”

“…The suspended particles were sonicated (10 minutes) and the light scattering with time at a definitive angle after interaction with particles measured. Samples were assessed at a temperature rate of 10 o C / min, in the range of 10 degree Celsius to 230 degree Celsius using DSC822e differential scanning calorimeter (DSC) [25][26][27][28] (Mettler Toledo, Polaris Parkway, USA) adopted to assess the degree of crystallinity and thermal properties.An empty pan was used as a reference. The X-ray diffraction (XRD) of the nanoformulations was assessed with Philips PW 1820/00 automated diffractometer with Cu-Kaα radiations to measure the crystal quality of the samples.…”

Fabrication and investigation potential effect of lentinan and docetaxel nanofibers for synergistic treatment of breast cancer in vitro

“…Risk factors such as gender (100 times more common in women than men), age, family history, unhealthy lifestyles, hormonal factors, reproductive factors, radiation, and gene mutations are involved in increasing the risk of breast cancer 4,5 . Breast cancer is a heterogeneous disease involving several different characteristics related to distinguishably histological and biological characteristics, clinical presentation and behaviors, with different responses to treatment 6 and the conventional approach for breast cancer therapy are chemotherapy, irradiation, and surgical excision 7 . According to the histological characteristics of breast cancer, it is divided into two categories: in situ and invasive carcinoma 8 .…”

“…4,5 Breast cancer is a heterogeneous disease involving several different characteristics related to distinguishably histological and biological characteristics, clinical presentation and behaviors, with different responses to treatment 6 and the conventional approach for breast cancer therapy are chemotherapy, irradiation, and surgical excision. 7 According to the histological characteristics of breast cancer, it is divided into two categories: in situ and invasive carcinoma. 8 Based on molecular and histological evidence, breast cancer is divided into three groups, including hormone receptor-expressing breast cancer (Estrogen receptor(ER+) or progesterone receptor (PR+)), breast cancer expressing human epidermal receptor 2 (HER2+), and Triple-negative breast cancer (TNBC) (ER-, PR-, and HER2-); which their treatment method is determined based on the molecular properties of cancer.…”

Erratum

“…In SreeHarsha et al, 1 errors were published in the author's affiliations. They are correctly listed below.…”