Paclitaxel quantification in mouse plasma and tissues containing liposome-entrapped paclitaxel by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetics study

A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

“…Moreover, the analysis run times were longer than 25 min makes the process laborious and time consuming which is not acceptable in routine HPLC analysis of PTX particularly it's pharmacokinetic and bioequivalence studies. Some other researchers achieved to the lower LOQ for PTX in plasma and tissues following liquid chromatography tandem mass spectrometric method (30)(31)(32). Although, these methods are sensitive and accurate with the short chromatographic time, they involve expensive equipment which is not affordable at most laboratories.…”

“…They used a small volume of plasma sample (100 µl) and intra-and inter-day variation of the assay were within 9.35 and 8.33%, respectively. To the best of our knowledge, protein precipitation of tissue homogenates following liquid chromatographic tandem mass spectrometric (LC-MS) method has been the only successful method for quantification of PTX in tissue samples (30)(31)(32). Though, LC-MS is more responsive and explicit than HPLC-UV, this technique involves expensive equipment which is not affordable at most laboratories.…”

Cancer-Specific Transgene Expression Mediated by Systemic Injection of Nanoparticles

“…These characteristics, combined with a poor lymphatic drainage, result in the fact that macromolecules with high blood circulation time and specific features such as charge and size will be preferentially accumulated in tumors. This accumulation has been shown to be restricted to high molecular weight macromolecules with a slow clearance, and this concept has given rise to at least 11 clinical trails testing the efficacy of polymer-drug conjugates (4,(18)(19)(20).…”

“…Another consideration that is key to the translation of PPGI3/ DNA complexes to the clinic is the biophysical characterization of the nanoparticles. Delivery of polymer-drug conjugates containing doxorubicin (18), paclitaxel (19), or camptothecin (20) to tumors has been described and some of these compounds are in phase III clinical trials (21). These complexes have been shown to remain for long periods in the blood stream and accumulate passively in tumor tissues through a phenomenon referred to as the enhanced permeability and retention effect (EPR).…”

Determination of paclitaxel and other six taxoids in Taxus species by high-performance liquid chromatography–tandem mass spectrometry

“…It is regarded as one of the most useful anticancer drugs of plant origin available today. It is effective in the treatment of many tumor types, such as ovarian, breast, melanoma, head, neck, bladder, gastrointestinal, lung cancer, and non-small-cell lung cancer [1][2][3], as well as for AIDS-related Kaposi's sarcoma [4][5][6]. Its fundamental anticancer mechanism is unique [7].…”