Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts

Abstract

Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).

Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia

“…In order to investigate the antibacterial activity of the root extracts, the modified broth microdilution method was used [ 15 , 16 ]. Bacterial species were cultured for 24 h at 37 °C in a Tryptic soy broth (TSB) medium.…”

“…Compounds 32 and 33 (gibberellin and phloridzin) were found in the trees P. ostii, P. rockii, and P. suffruticosa [38][39][40]. 16), 447 (29), 599 (34), 601 (22), 617 (100) 193 (10), 313 (15), 429 (33), 291 (66), 301 (20), 409 (60), 427 (100) 247 (14), 275 (62), 287 (18), 301 (69), 409 (100) 257 (20), 287 (100), 299 (10) 95 (26), 109 (42), 113 (26), 127 (28), 137 (100) 69 (47), 81 (25), 95 (39), 106 (7) (20), 313 (10), 399 (10), 479 (12), 491 (23), 613 (100) 271 (100), 313 (33), 375 (16), 399 (27), 491 (67) 169 (8), 210 (7), 211 (18), 285 (13), 287 (47), 313…”

“…The software Xcalibur (Xcalibur 2.1, Thermo Fisher Scientific, Waltham, MA, USA) was used for instrument control, data acquisition, and data analysis. Deprotonated molecule mass [M-H] − and MS 2 , MS 3 , and MS 4 fragmentation patterns were used for the identification of active compounds in the extract with the assistance of the available spectroscopic, accurate mass, and MS fragmentation data from the literature [15,16,19,20,93].…”

“…The root of Paeonia peregrina (PP) was a source of specific “cage-like” monoterpene glucosides, condensed tannins, and mono(di)-saccharides (e.g., aldoses) [ 14 ]. Even though the biological activity of PP is still very poorly investigated and refers only to the chemistry and biological potential of roots, flowers, and petals [ 15 , 16 ], its use for the treatment of epilepsy, spasms, and hemorrhage is documented [ 14 , 15 ]. Regarding the roots of Paeonia tenuifolia (PT), in the available literature, there is no published data about their biological activity.…”

Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential

“…In the past, various plant parts of peonies (seeds, flowers, leaves and roots, as well as whole plants) have been widely used to treat various diseases, pain (head, stomach, and eyes) [4], problem regarding female genitals (dysmenorrhea and amenorrhea) [5], aches, neurological diseases (spasm and epilepsy) [6], infectious diseases (carbuncles) [4], urinary system diseases, and inflammation (otitis media, appendicitis, and gastritis) [6], as well as trauma. The petals of herbaceous peonies have been a subject of very few studies, and it has been shown that they possess skin-beneficial biological activities [3,7].…”