Paeonol Ameliorates Ulcerative Colitis in Mice by Modulating the Gut Microbiota and Metabolites

Zheng, Jiaojiao; Li, Huan; Zhang, Pei; Yue, Shi‐Jun; Zhai, Bingtao; Zou, Junbo; Cheng, Jiangxue; Zhao, Chongbo; Guo, Dongyan; Wang, Jing

doi:10.3390/metabo12100956

Cited by 11 publications

(6 citation statements)

References 70 publications

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“…We screened the top 10 phyla and genera in terms of abundance and presented them on bar charts. Firmicutes and Bacteroidetes accounted for the largest proportion of phyla at the phylum level, which is consistent with previous studies (Liu, Zhou, et al, 2022; Zheng et al, 2022). And in the CTRL group, Firmicutes and Campylobacterota were more abundant at the phylum level (Figure 8a).…”

Section: Resultssupporting

confidence: 92%

Geniposidic acid attenuates DSS‐induced colitis through inhibiting inflammation and regulating gut microbiota

Jiang

Zhang

et al. 2023

Phytotherapy Research

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Geniposidic acid (GPA) is a bioactive compound isolated from Gardenia jasminoides Ellis (Rubiaceae) that has long been used to treat arthritis, jaundice, and hypertension.However, the therapeutic effects of GPA against colitis remain underexplored. This study aimed to investigate the effect of GPA on the remission of colitis and the underlying mechanisms. A DSS-induced colitis mouse model was used to evaluate the influence of GPA on the modulation of gut microbiota and intestinal epithelial barrier function. Our results indicated that GPA improved DSS-induced mouse colitis, including loss of body weight, disease activity index (DAI), colon length, and colonic pathological damage. DSS-induced destruction of the intestinal barrier was also significantly repaired by GPA treatment. In addition, the relative levels of proinflammatory cytokines, such as IL-1β and TNF-α, were markedly alleviated by GPA. Furthermore, western blot analysis revealed that GPA downregulated the protein expression of the nuclear transcription factor NF-κB. Finally, we first demonstrated that GPA could alleviate gut microbiota dysbiosis in mice with colitis by bacterial 16S rRNA sequencing. In conclusion, our study demonstrates the therapeutic and protective effects of GPA on IBD and provides novel insights into the prevention of colitis by targeting gut microbiota metabolism using natural products.

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Section: Resultssupporting

confidence: 92%

Geniposidic acid attenuates DSS‐induced colitis through inhibiting inflammation and regulating gut microbiota

Jiang

Zhang

et al. 2023

Phytotherapy Research

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“…The gut microbiota is well-known to play an essential role in improving the UC phenotypes. , For this reason, we evaluated the efficacy of NEPs versus EPs and DFs in improving body weight, DAI, colon length, spleen weight, and cecal weight of the tested UC mice (Figure A). It was found that the body weight and the colonic length of DSS-treated mice were significantly reduced when compared to NC mice (Figure B–D, p < 0.05), and this reduction was obviously restrained by consumption of NEPs ( p < 0.05), but the colitis mice supplemented with EPs or DFs did not exhibit this change.…”

Section: Resultsmentioning

confidence: 99%

“…Ileibacterium and Alloprevotella, a group of bacteria associated with SCFA formation, have been proved to modulate systemic inflammation. , The gut microbiome, known as the human second genome, has been proved to be closely related to the occurrence and development of UC . Many studies also indicated that the patients or mice with UC exhibited a significant disruption of intestinal microorganisms. ,, For these reasons, we evaluated whether NEPs could ameliorate gut microbiota disturbance to relieve UC symptoms, and a chronic colitis model was established by drinking 1.5% DSS water in C57/B6J mice. The UC symptoms and colonic damage are approved to be highly similar between the patients and mice, , while supplementation of NEPs could significantly improve these symptoms, which was followed by DFs, and this similar effect could not be achieved by EP treatment, indicating that NEPs improved the DSS-induced UC symptoms by regulating the structure of gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

Colon-Targeted Release of Turmeric Nonextractable Polyphenols and Their Anticolitis Potential via Gut Microbiota-Dependent Alleviation on Intestinal Barrier Dysfunction in Mice

Yang

et al. 2023

J. Agric. Food Chem.

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Solid evidence has emerged supporting the role of nonextractable polyphenols (NEPs) and dietary fibers (DFs) as gut microbiota modulators. This study aims to elucidate gut microbiota-dependent release of turmeric NEPs and examine the possible anti-inflammatory mechanism in the dextran sulfate sodium-induced ulcerative colitis (UC) model. 1.5% DSS drinking water-induced C57BL/6J mice were fed a standard rodent chow supplemented with or without 8% extractable polyphenols (EPs), NEPs, or DFs for 37 days. The bound curcumin, demethoxycurcumin, and bisdemethoxycurcumin in NEPs were released up to 181.5 ± 10.6, 65.2 ± 6.0, and 69.5 ± 7.6 μg/mL by in vitro gut microbiota-simulated fermentation and released into the colon of NEP-supplemented mice by 5.7-, 11.0-, and 7.8-fold higher than pseudo germ-free mice, respectively (p < 0.05). NEPs also enhanced the colonic microbiota-dependent production of short-chain fatty acids in vitro and in vivo (p < 0.05). Interestingly, NEP feeding significantly improved the DSS-caused gut microbiota disorder, epithelial barrier damage, and inflammation of UC mice better than EPs or DFs (p < 0.05). Meanwhile, the pseudo germ-free mice supplemented with NEPs failed to ameliorate UC symptoms. These findings manifest that turmeric NEPs as macromolecular carriers exert the target delivery of polyphenols into the colon for regulating gut microbiota to restore the impaired gut barrier function for alleviation of inflammation.

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“…animalis F1–7 reduced Turicibacter and Clostridium_sensu_stricto_1 . The abundance of Romboutsia was abnormally increased in the intestines of inflamed mice . F1–7 intervention reduced the abundance of Romboutsia , implying the weakening of the inflammatory response.…”

Section: Discussionmentioning

confidence: 95%

Bifidobacterium animalis subsp. lactis F1–7 Alleviates Lipid Accumulation in Atherosclerotic Mice via Modulating Bile Acid Metabolites to Downregulate Intestinal FXR

Liang,

Zheng,

Wang

et al. 2024

J. Agric. Food Chem.

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The dysfunction of intestinal microbiota and bile acid metabolism is related to the pathogenesis of atherosclerosis. This study we explored the mechanism of Bif idobacterium animalis subsp. lactis F1−7 (Bif. animalis F1−7), improving atherosclerosis by regulating the bile acid metabolism and intestinal microbiota in the ApoE −/− mice. The Bif. animalis F1−7 effectively reduced aortic plaque accumulation and improved the serum and liver lipid levels in atherosclerotic mice. The untargeted metabolomics revealed that Bif. animalis F1−7 reduced the glycine-conjugated bile acids and the levels of differential metabolite lithocholic acid (LCA) significantly. Downregulation of LCA decreased the intestinal levels of the farnesoid X-activated receptor (FXR) and regulated the bile acid metabolism through the FXR/FGF15/CYP7A1 pathway. Furthermore, the 16srRNA gene sequencing analysis revealed that structural changes in intestinal microbiota with an increase in the abundance of Bif idobacterium, Lactobacillus, Faecalibaculum, Desulfovibrio, and a decrease in Dubosiella, Clostridium_sensu_stricto_1, and Turicibacter following the Bif. animalis F1−7 intervention. Correlation analysis showed that the changes in intestinal microbiota mentioned above were significantly correlated with bile acid metabolism in atherosclerotic mice. In conclusion, this study sheds light on the mechanisms by which Bif. animalis F1−7 regulates atherosclerosis.

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Paeonol Ameliorates Ulcerative Colitis in Mice by Modulating the Gut Microbiota and Metabolites

Cited by 11 publications

References 70 publications

Geniposidic acid attenuates DSS‐induced colitis through inhibiting inflammation and regulating gut microbiota

Geniposidic acid attenuates DSS‐induced colitis through inhibiting inflammation and regulating gut microbiota

Colon-Targeted Release of Turmeric Nonextractable Polyphenols and Their Anticolitis Potential via Gut Microbiota-Dependent Alleviation on Intestinal Barrier Dysfunction in Mice

Bifidobacterium animalis subsp. lactis F1–7 Alleviates Lipid Accumulation in Atherosclerotic Mice via Modulating Bile Acid Metabolites to Downregulate Intestinal FXR

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