PAL31, a Nuclear Protein Required for Progression to the S Phase

Sun, Weiyong; Hattori, Naka; Mutai, Hideki; Toyoshima, Yuka; Kimura, Hiromichi; Tanaka, Satoshi; Shiota, Kunio

doi:10.1006/bbrc.2000.4244

Cited by 23 publications

(19 citation statements)

References 27 publications

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“…S3). Similarly, prompted by the conclusions of previous reports (15,17), we investigated whether loss of ANP32B affected cell proliferation, but, again, no defects in in vitro proliferation were found for Anp32b −/− primary MEFs, thymocytes, or splenocytes (Fig. S4).…”

Section: Resultsmentioning

confidence: 93%

“…Reports that ANP32B expression was related to cell proliferation (15,17) prompted us to investigate whether ANP32B expression was altered in human tumor samples. We examined the relationship between ANP32B mRNA expression and breast cancer patient prognosis using information from three available studies, namely the NEJM (New England Medical Journal) (37), the BMC (BMC Genomics) (38), and the PNAS datasets (39).…”

Section: Resultsmentioning

confidence: 99%

“…The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation (4-7), apoptotic cell death (8)(9)(10)(11)(12)(13)(14), and cell proliferation (15)(16)(17). Diverse mechanisms have been postulated for how these proteins perform their function(s).…”

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confidence: 99%

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Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development

Reilly

Afzal²,

Gorrini³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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The highly conserved ANP32 proteins are proposed to function in a broad array of physiological activities through molecular mechanisms as diverse as phosphatase inhibition, chromatin regulation, caspase activation, and intracellular transport. On the basis of previous analyses of mice bearing targeted mutations of Anp32a or Anp32e, there has been speculation that all ANP32 proteins play redundant roles and are dispensable for normal development. However, more recent work has suggested that ANP32B may in fact have functions that are not shared by other ANP32 family members. Here we report that ANP32B expression is associated with a poor prognosis in human breast cancer, consistent with the increased levels of Anp32b mRNA present in proliferating wild-type (WT) murine embryonic fibroblasts and stimulated WT B and T lymphocytes. Moreover, we show that, contrary to previous assumptions, Anp32b is very important for murine embryogenesis. In a mixed genetic background, ANP32B-deficient mice displayed a partially penetrant perinatal lethality that became fully penetrant in a pure C57BL/6 background. Surviving ANP32B-deficient mice showed reduced viability due to variable defects in various organ systems. Study of compound mutants lacking ANP32A, ANP32B, and/or ANP32E revealed previously hidden roles for ANP32A in mouse development that became apparent only in the complete absence of ANP32B. Our data demonstrate a hierarchy of importance for the mammalian Anp32 genes, with Anp32b being the most critical for normal development.cell growth | mouse embryogenesis | premature aging

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development

Reilly

Afzal²,

Gorrini³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…PAL31 has high similarity to April (acidic protein rich in leucines). It is a nuclear protein presumably associated with cell cycle progression [Sun et al, 2001]. PAL31 is expressed in the entire embryonic brain but is only found in a few places in the adult brain where neural progenitor cells are located.…”

Section: Known Gene Productsmentioning

confidence: 99%

Identification of Differentially Expressed cDNA Clones from Gerbil Cochlear Outer Hair Cells

Zheng

Long²,

Robison³

et al. 2002

Audiol Neurotol

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In order to identify genes that are associated with outer hair cell(OHC)-specific function, a plasmid library enriched with OHC-specific gene products was constructed using single cell-type-specific complementary DNA (cDNA) and a PCR subtractive hybridization strategy. As a first step, we created separate OHC and inner hair cell (IHC) cDNA pools from individually collected cells using a nonspecific reverse transcription polymerase chain reaction. Next, the OHC cDNA was subtracted against IHC cDNA using a PCR-based subtractive technique. IHCs and OHCs share many common features, making IHC cDNA an ideal ‘driver’ to ‘subtract away’ common hair cell gene products and enrich differentially expressed cDNAs, including OHC-specific genes. The subtracted OHC cDNAs were then cloned to generate an OHC – IHC subtracted cDNA plasmid library. Finally, a differential screening procedure was performed, resulting in 477 differentially positive clones. After analysis of these 477 clones, 50 known genes were identified, including two previously known OHC-specific proteins: oncomodulin and the recently described motor protein prestin. An additional 84 novel clones were also found. As this library of cDNA fragments represents differentially expressed genes in OHCs, it can be used as starting material for isolation and characterization of a complete set of OHC gene products, an important step in investigating normal and abnormal cochlear function.

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“…Expression of PAL31 is also observed in the subventricular zone in adult brain, which contains neuro-precursor cells (1). In addition, the protein is colocalized with proliferating cell nuclear antigen (PCNA) in the nuclei of neural and Nb2 lymphoma cells (7), suggesting that PAL31 is a nuclear protein related to DNA synthesis.…”

mentioning

confidence: 98%