Palmitic acid accumulation limits methane production in anaerobic co-digestion of fats, oils and grease with municipal wastewater sludge

Deaver, Jessica A.; Diviesti, Karla I.; Soni, Mehul N.; Campbell, Barbara J.; Finneran, Kevin T.; Popat, Sudeep C.

doi:10.1016/j.cej.2020.125235

Cited by 33 publications

(12 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The syntrophic lifestyle of the species of Candidatus Cloacimonas was previously suggested based on identification of genes related to syntrophic propionate oxidation in the presence of hydrogen-utilizing microorganisms [ 49 ]. In addition, the occurrence of members of phylum Cloacimonetes (e.g., Cloacimonadales W27, Cloacidonadaceae W5 , and Candidatus Cloacimonas ) during anaerobic lipids and LCFA degradation in different anaerobic environments has been used as an argument for their potential involvement in lipid degradation [ 16 , 50 , 51 ], which is in line with our observation on the prevalence of Candidatus Cloacimonas in R2 following oleate pulse feeding. Nevertheless, Candidatus Cloacimonas had the lowest degree, betweenness, and closeness centrality among the bacteria in the co-occurrence network, suggesting that this genus had limited interactions with other microorganisms [ 35 ] and, therefore, its contribution to the integrity and functions of the microbial community is possibly marginal.…”

Section: Discussionsupporting

confidence: 86%

Effluent solids recirculation to municipal sludge digesters enhances long-chain fatty acids degradation capacity

Yekta

Liu

Anacleto

et al. 2021

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. In this study, we evaluated the effect of effluent solids recirculation on microbial LCFA (oleate) degradation capacity in continuous stirred-tank sludge digesters, with the overall aim of providing operating conditions for efficient co-digestion of waste lipids. Furthermore, the impacts of LCFA feeding frequency and sulfide on process performance and microbial community dynamics were investigated, as parameters that were previously shown to be influential on LCFA conversion to biogas. Results Effluent solids recirculation to municipal sludge digesters enabled biogas production of up to 78% of the theoretical potential from 1.0 g oleate l−1 day−1. In digesters without effluent recirculation, comparable conversion efficiency could only be reached at oleate loading rates up to 0.5 g l−1 day−1. Pulse feeding of oleate (supplementation of 2.0 g oleate l−1 every second day instead of 1.0 g oleate l−1 every day) did not have a substantial impact on the degree of oleate conversion to biogas in the digesters that operated with effluent recirculation, while it marginally enhanced oleate conversion to biogas in the digesters without effluent recirculation. Next-generation sequencing of 16S rRNA gene amplicons of bacteria and archaea revealed that pulse feeding resulted in prevalence of fatty acid-degrading Smithella when effluent recirculation was applied, whereas Candidatus Cloacimonas prevailed after pulse feeding of oleate in the digesters without effluent recirculation. Combined oleate pulse feeding and elevated sulfide level contributed to increased relative abundance of LCFA-degrading Syntrophomonas and enhanced conversion efficiency of oleate, but only in the digesters without effluent recirculation. Conclusions Effluent solids recirculation improves microbial LCFA degradation capacity, providing possibilities for co-digestion of larger amounts of waste lipids with municipal sludge.

show abstract

Section: Discussionsupporting

confidence: 86%

Effluent solids recirculation to municipal sludge digesters enhances long-chain fatty acids degradation capacity

Yekta

Liu

Anacleto

et al. 2021

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Results obtained by Deaver et al. (2020) suggest that palmitic acid conversion likely limits grease trap waste codigestion with sludge in inhibited or stalled digesters.…”

Section: Resultsmentioning

confidence: 97%

“…Palmitic acid is less toxic than oleic acid and can be directly generated from oleic and linoleic acids, but it depends on biological activity. Results obtained by Deaver et al (2020) suggest that palmitic acid conversion likely limits grease trap waste codigestion with sludge in inhibited or stalled digesters.…”

Section: Addition Of Raw and Pretreated Sugarcane Bagassementioning

confidence: 98%

Methane production from anaerobic digestion of dairy grease trap waste: Effect of sugarcane bagasse addition

Bueno

Henao

Rabelo

et al. 2021

Environmental Quality Mgmt

View full text Add to dashboard Cite

Lipids are attractive substrates for anaerobic digestion due to their high methane production yield. However, high concentrations of lipids may cause problems in anaerobic processes. Typically, grease trap waste is mechanically removed and discharged, both generating environmental issues and wasting a potential energy source. In this study, grease trap waste from a dairy wastewater treatment plant was subjected to anaerobic digestion. Untreated and pretreated sugarcane bagasse was evaluated as fat adsorbents to reduce the inhibitory effect of the fatty waste. The bagasse pretreated by the organosolv method was the condition that most contributed to increased methane production (82%) from anaerobic digestion of dairy fatty waste at 0.5 g COD.g–1TVS concentration. Untreated bagasse provided the second‐best condition, followed by hydrothermally treated bagasse, presenting no statistical difference. The obtained results showed the potential of using sugarcane bagasse as a strategy to reduce the inhibitory effect of the fatty waste from the dairy grease trap, making this waste another possible source for generating biogas.

show abstract

“…No information was found regarding the concentrations of the other three compounds in wastewater. Palmitelaidic acid was reported to be used to produce cosmetics, soaps, and industrial mold release agents [58]. and the average concentrations were 353.4 ± 51.2 µg/L and 478.2 ± 62 µg/L in the control and suppression facilities, respectively.…”

Section: Resultsmentioning

confidence: 99%

Identification and Quantification of Bioactive Compounds Suppressing SARS-CoV-2 Signals in Wastewater-based Epidemiology Surveillance

Bayati

Hsieh

Hsu

et al. 2022

Preprint

View full text Add to dashboard Cite

Recent SARS-CoV-2 wastewater-based epidemiology (WBE) surveillance have documented a positive correlation between the number of COVID-19 patients in a sewershed and the level of viral genetic material in the wastewater. Efforts have been made to use the wastewater SARS-CoV-2 viral load to predict the infected population within each sewershed using a multivariable regression approach. However, reported clear and sustained variability in SARS-CoV-2 viral load among treatment facilities receiving industrial wastewater have made clinical prediction challenging. Several classes of molecules released by regional industries and manufacturing facilities, particularly the food processing industry, can significantly suppress the SARS-CoV-2 signals in wastewater by breaking down the lipid-bilayer of the membranes. Therefore, a systematic ranking process in conjugation with metabolomic analysis was developed to identify the wastewater treatment facilities exhibiting SARS-CoV-2 suppression and identify and quantify the chemicals suppressing the SARS-COV-2 signals. By ranking the viral load per diagnosed case among the sewersheds, we successfully identified the wastewater treatment facilities in Missouri, USA that exhibit SARS-CoV-2 suppression (significantly lower than 5 X 10^11 gene copies/reported case) and determined their suppression rates. Through both untargeted global chemical profiling and targeted analysis of wastewater samples, 40 compounds were identified as candidates of SARS-CoV-2 signal suppression. Among these compounds, 14 had higher concentrations in wastewater treatment facilities that exhibited SARS-CoV-2 signal suppression compared to the unsuppressed control facilities. Stepwise regression analyses indicated that 4-nonylphenol, palmitelaidic acid, sodium oleate, and polyethylene glycol dioleate are positively correlated with SARS-CoV-2 signal suppression rates. Suppression activities were further confirmed by incubation studies, and the suppression kinetics for each bioactive compound were determined. According to the results of these experiments, bioactive molecules in wastewater can significantly reduce the stability of SARS-CoV-2 genetic marker signals. Based on the concentrations of these chemical suppressors, a correction factor could be developed to achieve more reliable and unbiased surveillance results for wastewater treatment facilities that receive wastewater from similar industries.

show abstract

Palmitic acid accumulation limits methane production in anaerobic co-digestion of fats, oils and grease with municipal wastewater sludge

Cited by 33 publications

References 47 publications

Effluent solids recirculation to municipal sludge digesters enhances long-chain fatty acids degradation capacity

Effluent solids recirculation to municipal sludge digesters enhances long-chain fatty acids degradation capacity

Methane production from anaerobic digestion of dairy grease trap waste: Effect of sugarcane bagasse addition

Identification and Quantification of Bioactive Compounds Suppressing SARS-CoV-2 Signals in Wastewater-based Epidemiology Surveillance

Contact Info

Product

Resources

About